Results 41 to 50 of about 165,447 (318)

Co‐overexpression of the caloric restriction‐induced mitochondrial factors PGC‐1α and MIPEP upregulates Phospho1 expression in adipocytes

FEBS Open Bio, EarlyView.
Caloric restriction that extends lifespan induces the expression of PGC‐1α and MIPEP in white adipose tissue. In this study, co‐overexpression of Pgc‐1α and Mipep upregulated the gene expression of PHOSPHO1. These findings provide new insights into mitochondria‐related mechanisms underlying the effects of caloric restriction in adipocytes.
Mamiko Ishimatsu, Kanari Taki, Asuka Hayami, Komachi Kato, Yuka Nozaki, Yuhei Mizunoe, Takumi Narita, Ryoichi Mori, Yoshikazu Higami, Masaki Kobayashi   +9 more
wiley   +1 more source

Substrate-assisted catalysis in the cleavage of DNA by the EcoRI and EcoRV restriction enzymes.

Proceedings of the National Academy of Sciences of the United States of America, 1993
The crystal structure analyses of the EcoRI-DNA and EcoRV-DNA complexes do not provide clear suggestions as to which amino acid residues are responsible for the activation of water to carry out the DNA cleavage.
A. Jeltsch, J. Alves, H. Wolfes, Günter Maass, A. Pingoud   +4 more
semanticscholar   +1 more source

Topoisomerase I Inhibition in ETV4‐overexpressed Non‐Small Cell Lung Cancer Promotes Replication and Transcription Mediated R‐Loop Accumulation and DNA Damage

Advanced Science, EarlyView.
The oncogenic ETS factor ETV4 exerts a pleiotropic control over DNA replication both in a transcription‐dependent and ‐independent fashion in NSCLC cells. High‐ETV4 expression leads to R‐loop formation and DNA damage in response to TOP1 inhibition.
Jiaxi Zhang, Yan Wang, Shanhu Cao, Shelly M. Xie, Bei Liu, Yimeng Li, Yuqi Hou, Xue Meng, Mingzhu Ruan, Di Bu, Jia Kang, Ruxin Li, Lei Lou, Juan Wang, Lingxiao Xing   +14 more
wiley   +1 more source

Method for Cloning Restriction Fragments Containing the Termini of BAC Inserts

BioTechniques, 2000
We have developed a method to isolate the termini of BAC clones. The method is based on the two unique NotI sites located approximately 300 bp on either side of the EcoRI cloning site of the BAC vector pECSBAC4. Our strategy includes the following steps:
Tammy R. Plyler, C. Eduardo Vallejos
doaj   +1 more source

Macronutrient intake modulates impact of EcoRI polymorphism of ApoB gene on lipid profile and inflammatory markers in patients with type 2 diabetes

Scientific Reports, 2022
We sought to examine whether dietary intakes may affect the relationship between ApoB EcoRI and lipid profile, as well as serum inflammatory markers, in patients with type 2 diabetes (T2DM). This current study consisted of 648 diabetic patients.
Faezeh Abaj, Fariba Koohdani
doaj   +1 more source

Loop-mediated isothermal amplification assay for the rapid detection of Anaplasma marginale in cattle based on major surface protein 5 gene

Journal of Vector Borne Diseases, 2023
Background & objectives: Timely intervention is needed to minimize the economic losses of vector-borne bovine anaplasmosis which can be possible by the isothermal amplification assay. Methods: Anaplasma marginale in the cattle of south Gujarat, India was
Nabanita Ganguly, Niranjan Kumar, Jayesh B Solanki, I H Kalyani, Nabanita Thakuria, Dharmesh C Patel   +5 more
doaj   +1 more source

Potent Cross‐neutralizing Antibodies Reveal Vulnerabilities of Henipavirus Fusion Glycoprotein

Advanced Science, EarlyView.
Hendra and Nipah viruses (HNVs) pose significant threats to global health. This work reports potent cross‐neutralizing antibodies targeting the fusion glycoprotein (F) and reveals shared features of these antibodies and vulnerabilities of F, thereby guiding the rational design of countermeasures against HNVs and similar pathogens.
Yi Ren, Pengfei Fan, Xinghai Zhang, Ting Fang, Zhengshan Chen, Yanfeng Yao, Xiangyang Chi, Guanying Zhang, Xiaofan Zhao, Bingjie Sun, Fangxu Li, Zixuan Liu, Zhenwei Song, Baoyue Zhang, Cheng Peng, Entao Li, Yilong Yang, Jianmin Li, Sandra Chiu, Changming Yu   +19 more
wiley   +1 more source

Genome-wide mapping using new AFLP markers to explore intraspecific variation among pathogenic Sporothrix species.

PLoS Neglected Tropical Diseases, 2020
Sporotrichosis is a chronic subcutaneous mycosis caused by Sporothrix species, of which the main aetiological agents are S. brasiliensis, S. schenckii, and S. globosa.
Jamile Ambrósio de Carvalho, Ferry Hagen, Matthew C Fisher, Zoilo Pires de Camargo, Anderson Messias Rodrigues   +4 more
doaj   +1 more source

The sequence dependent search mechanism of EcoRI [PDF]

, 2018
ABSTRACTOne-dimensional search is an essential step in DNA target recognition. Theoretical studies have suggested that the sequence dependence of one-dimensional diffusion can help resolve the competing demands of fast search and high target affinity, a conflict known as the speed-selectivity paradox.
Piatt, S.C., Loparo, J.J., Price, A.C.
openaire   +2 more sources

EcoRI analysis of bacteriophage P22 DNA packaging [PDF]

Journal of Molecular Biology, 1978
Abstract Bacteriophage P22 linear DNA molecules are a set of circularly permuted sequences with ends located in a limited region of the physical map. This mature form of the viral chromosome is cut in headful lengths from a concatemeric precursor during DNA encapsulation.
Jackson, Ethel Noland, Jackson, David A., Deans, Robert J.   +2 more
openaire   +4 more sources