Overmethylation of DNAs by the EcoRI methylase
Nucleic Acids Research, 1978 EcoRI methylase is able to catalyze methy incorporation into DNA at sequences other than the canonical EcoRI site. At high enzyme concentrations and over a wide range of pH and ionic strengths, EcoRI methylase modifies polyoma DNA (which contains one EcoRI site) at a number of sites.
K L, Berkner, W R, Folk
openaire +3 more sources
Advanced Science, EarlyView.An integrated approach combining mass spectrometry imaging, transcriptomics, and phylogenetic analysis facilitated the efficient identification of genes involved in gallotannin biosynthesis in Paeonia suffruticosa. As a result, a key UGT and several SCPL acyltransferases responsible for biosynthesizing pentagalloylglucose were successfully identified ...
Yushi Liu +4 more
wiley +1 more source
Use of AFLP marker system on sugarcane somaclones to study their resistance to rust
Biotecnología Vegetal, 2002 AFLPs (amplified fragment length polymorphism) was carried out from genomic DNA of five rust resistant sugar cane somaclons and their susceptible donor, Saccharum officinarum var B 4362, using three combinations of primers (EcoRI/aca: MseI/acc; EcoRI/aca:
María Ileana Oloriz +6 more
doaj
ISOLATION AND SEQUENCING OF A GENOMIC DNA ENCODING FOR ASCORBATE OXIDASE, A KEY ENZYME INVOLVED IN THE BIODEGRADATION OF ASCORBIC ACID IN MELON [PDF]
Journal of Central European Agriculture, 2002 A melon genomic library was used to isolate and characterize a clone of genomic DNA coding for ascorbate oxidase (AO) which is considered a key enzyme in the biodegradation of ascorbic acid (AA).
Evangelos ALATSATIANOS +5 more
doaj
BacS: An Abundant Bacteroid Protein in \u3cem\u3eRhizobium etli\u3c/em\u3e Whose Expression Ex Planta Requires \u3cem\u3enifA\u3c/em\u3e [PDF]
, 2003 Rhizobium etli CFN42 bacteroids from bean nodules possessed an abundant 16-kDa protein (BacS) that was found in the membrane pellet after cell disruption. This protein was not detected in bacteria cultured in tryptone-yeast extract.
Davila, Guillermo +3 more
core +1 more source
Advanced Science, EarlyView.This study identifies OCIAD2 as a critical regulator of cisplatin resistance in HNSCC. Mechanistically, OCIAD2 stabilizes integrin β1 through a direct physical interaction and facilitates its SNX17‐dependent endosomal recycling to lipid raft microdomains. Targeting OCIAD2 disrupts integrin β1 trafficking and significantly enhances cisplatin sensitivity,
Li Cui +9 more
wiley +1 more source
Advanced Science, EarlyView.A miniaturized deaminase SsdAtox was scanned with AlphaFold to identify DNA binding pocket hot spots. Site‐saturation mutagenesis at gatekeeper residue K31 yielded ten‐fold activity enhancement. Trinity Screen, an E. coli‐based three‐in‐one platform selecting for high activity and reduced double‐strand breaks, enabled combinatorial evolution at DNA ...
Ryeo Gang Son +2 more
wiley +1 more source
Verifikasi cDNA T29 sebagai kandidat gen pengkode protein Toxoplasma gondii dengan metode SDS PAGE
Journal of Biological Researches, 2012 The process of cDNA construction from mRNA isolated from Toxoplasma gondii has been done. There were 7 candidates cDNA which one of them is called T29.
Ira Djajanegara +3 more
doaj +1 more source
Cosmid Cloning of Five \u3cem\u3eZymomonas trp\u3c/em\u3e Genes by Complementation of \u3cem\u3eEscherichia coli\u3c/em\u3e and \u3cem\u3ePseudomonas putida trp\u3c/em\u3e Mutants [PDF]
, 1988 A library of Zymomonas mobilis genomic DNA was constructed in the broad-host-range cosmid pLAFR1. The library was mobilized into a variety of Escherichia coli and Pseudomonas putida trp mutants by using the helper plasmid pRK2013. Five Z.
Eddy, Christina Kay +2 more
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The ubiK protein is an accessory factor necessary for bacterial Ubiquinone (UQ) biosynthesis and forms a complex with the UQ biogenesis factor UbiJ [PDF]
, 2017 Ubiquinone (UQ), also referred to as coenzyme Q, is a widespread lipophilic molecule in both prokaryotes and eukaryotes in which it primarily acts as an electron carrier.
Aussel, Laurent +7 more
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