Results 71 to 80 of about 165,447 (318)
Revista Mexicana de Ciencias Pecuarias, 2012 Se exploró y caracterizó la diversidad fenotípica y genotípica de poblaciones del pasto punta blanca [Digitaria californica (Benth.) Henr.], en pastizales de Chihuahua. Se realizó análisis de componentes principales y conglomerados.
Carlos Raúl Morales Nieto +4 more
doaj
Analysis of DNA methylation in various swine tissues. [PDF]
PLoS ONE, 2011 DNA methylation is known to play an important role in regulating gene expression during biological development and tissue differentiation in eukaryotes. In this study, we used the fluorescence-labeled methylation-sensitive amplified polymorphism (F-MSAP)
Chun Yang +7 more
doaj +1 more source
Journal of Basic Microbiology, EarlyView.ABSTRACT Rhizorhabdus wittichii RW1 is known for its ability to degrade polycyclic aromatic hydrocarbons, such as dibenzo‐p‐dioxin (DD) and dibenzofuran (DF). We hypothesized that the R. wittichii RW1 benzoate 1,2‐dioxygenase shares electron transfer components with the DD/DF angular dioxygenase (DxnA1A2), similar to many aromatic hydrocarbon degrading
Igor Ivanovski +2 more
wiley +1 more source
Indonesian Journal of Biotechnology, 2019 Recombinant human epidermal growth factor (rh-EGF) has high value in therapies for h-EGF deficiency-related diseases. The expression of the h-EGF gene was designed by using the pET21b(+) vector and Escherichia coli BL21(DE3) as the expression host.
Achmad Rodiansyah +3 more
doaj +1 more source
Facilitated distortion of the DNA site enhances EcoRI endonuclease-DNA recognition.
Proceedings of the National Academy of Sciences of the United States of America, 1993 We have measured the binding of EcoRI endonuclease to a complete set of purine-base analogue sites, each of which deletes one functional group that forms a hydrogen bond with the endonuclease in the canonical GAATTC complex.
D. Lesser +4 more
semanticscholar +1 more source
ChemistryOpen, EarlyView.Two genetically modified strains are designed and constructed to produce 22‐hydroxy‐23,24‐bisnorchol‐4‐ene‐3‐one (4‐HBC) and 3‐oxo‐4,17‐pregnadiene‐20‐carboxylic acid methyl ester (PDCE) by disrupting and overexpressing the aldolase gene (thl) in the genetically modified strains of Mycolicibacterium neoaurum NRRL B‐3805, respectively, which are useful ...
Xuemei Li +8 more
wiley +1 more source
A Dodecapeptide Comprising the Extended Chain-α4 Region of the Restriction Endonuclease EcoRI Specifically Binds to the EcoRI Recognition Site [PDF]
Journal of Biological Chemistry, 1995 The restriction endonuclease EcoRI binds and cleaves DNA containing GAATTC sequences with high specificity. According to the crystal structure, most of the specific contacts of the enzyme to the DNA are formed by the extended chain region and the first turn of alpha-helix alpha 4 (amino acids 137-145).
Claus Urbanke +7 more
openaire +2 more sources
Small, EarlyView.This study presents a modular platform that integrates engineered proteins with DNA nanostructures to create dynamic, multifunctional materials. By combining orthogonal protein‐protein and protein‐DNA conjugation strategies, the authors demonstrate controlled self‐assembly into nanofibers and nanorods, along with reversible enzyme activation.
Weijun Zhou +4 more
wiley +1 more source
Modulating Restriction Endonuclease Activities and Specificities Using Neutral Detergents
BioTechniques, 1999 It is well known that type II restriction enzyme activities and specificities can be modulated by altering solution conditions. The addition of co-solvents such as dimethyl sulfoxide (DMSO), alcohols and polyols can promote star activity, which is the ...
Lori H. Conlan +3 more
doaj +1 more source
A new EcoRI polymorphism for the insulin receptor gene
Nucleic Acids Research, 1989 A 550 bp BamHI-Pst I fragment encompassing bp 1,926-2,476 of the human insulin receptor cDNA was obtained. EcoRI identifies a two allele polymorphism, with bands of 5.8 and 5.5 kb. Co-dominant segregation was demonstrated in one Venezuelan pedigree.
S.C. Elbein +3 more
openaire +4 more sources