Results 81 to 90 of about 223,024 (272)

Modulating Restriction Endonuclease Activities and Specificities Using Neutral Detergents

BioTechniques, 1999
It is well known that type II restriction enzyme activities and specificities can be modulated by altering solution conditions. The addition of co-solvents such as dimethyl sulfoxide (DMSO), alcohols and polyols can promote star activity, which is the ...
Lori H. Conlan, Thomas J. José, Kevin C. Thornton, Cynthia M. Dupureur   +3 more
doaj   +1 more source

POU2F1 Promotes Chemoresistance in Colorectal Cancer Cells via Attenuates the MDR2 Degradation Mediated by PPP1R11 Lactylation

Advanced Science, EarlyView.
Schematic diagram of our study showing that highly expressed POU2F1 in colorectal cancer (CRC) can directly accelerate the cytosolic lactate export to decrease PPP1R11 lactylation at K59, thereby attenuating the E3 ligase enzyme activity and protein stability of PPP1R11, which inhibits the ubiquitination of MDR2 at K413 and K538 but increases the ...
Longzheng Xia, Jinguan Lin, Xianjie Jiang, Linda Oyang, Shiming Tan, Zongyao Ren, Qiu Peng, Qianjin Liao, Yujuan Zhou   +8 more
wiley   +1 more source

Targets for the MalI repressor at the divergent Escherichia coliK-12malX-malI promoters [PDF]

, 2010
Random mutagenesis has been used to identify the target DNA sites for the MalI repressor at the divergent Escherichia coli K-12 malX-malI promoters. The malX promoter is repressed by MalI binding to a DNA site located from position -24 to position -9 ...
Barnard, Barne, Busby, Chaudhuri, Clausen, El-Robh, Georgina S. Lloyd, Hollands, Jacob, Lloyd, Lodge, Mitchell, Mitchell, Reidl, Reidl, Rita E. Godfrey, Rojo, Stephen J.W. Busby, Swint-Kruse, Valentin-Hansen, Weickert, West, Zdych   +22 more
core   +1 more source

CircRSU1 Activates the hnRNPA1/HIF‐1α/CD24 Signaling Axis, Promoting Stemness Features of Hepatocellular Carcinoma

Advanced Science, EarlyView.
This study reveals circRSU1 with important oncogenic roles in hepatocellular carcinoma (HCC). CircRSU1, highly abundant in HCC, interacts with and stabilizes hnRNPA1 from ubiquitination and degradation. This stabilization facilitates hnRNPA1 binding to the internal ribosome entry site of HIF1A to increase HIF‐1α protein translation.
Shuting Xue, Danduo Wei, Yongzhi Zhao, Jinchang Pan, Hanyuan Zhang, Niya Liu, Jianjuan Zhang, Jiaxin Liu, Jianping Jin, Mu Xiao, Xinhua Feng, Aifu Lin, Lei Zhao, Stephanie Roessler, Junfang Ji   +14 more
wiley   +1 more source

A Simple and Adaptable Method for Cloning Genes Into Transposon Vectors Using Topo and Restriction Systems for Chicken Embryo Transgenesis

Bio-Protocol
Transposon-based genetic transformation enables stable transgene integration in avian genomes and is increasingly used in the development of transgenic chickens for enhanced disease resistance, productivity, and biopharmaceutical applications ...
Pamela Kirimi, Noah Okumu, John Maingi, Joseph Ngeranwa, Philip Nyaga, Yatinder Binepal   +5 more
doaj   +1 more source

MicroRNA‐Seq Analysis Reveals a Critical Role for miR‐222 in Bovine Intramuscular Adipogenesis via the DDIT4/mTORC1 Axis

Animal Research and One Health, EarlyView.
Schematic diagram illustrating how miR‐222 promotes bovine intramuscular adipogenesis by targeting DDIT4 to activate the mTORC1 signaling pathway. ABSTRACT Intramuscular fat (IMF) is a key determinant of beef quality, particularly with respect to tenderness, flavor, and juiciness.
Jianbing Tan, Liang Qiu, Zhenghai Zhou, Zhipeng Zhang, Nanfei Wang, Jiangang Hao, Linsen Zan, Wucai Yang   +7 more
wiley   +1 more source

Modular Nucleic Acid Assembled p/MHC Microarrays for Multiplexed Sorting of Antigen-Specific T Cells [PDF]

, 2009
The human immune system consists of a large number of T cells capable of recognizing and responding to antigens derived from various sources. The development of peptide-major histocompatibility (p/MHC) tetrameric complexes has enabled the direct ...
Bailey, Ryan C., Comin-Anduix, Begonya, Hadrup, Sine Reker, Heath, James R., Hwang, Kiwook, Koya, Richard C., Kwong, Gabriel A., Ma, Chao, Radu, Caius G., Ribas, Antoni, Schumacher, Ton N., Shu, Chengyi J., Witte, Owen N.   +12 more
core   +2 more sources

The transformer gene controls sexual development in Drosophila suzukii

Insect Science, EarlyView.
The transformer gene plays a key role in the genetic pathway for sexual development in Drosophila suzukii Abstract The genetic network of sex determination in the model organism Drosophila melanogaster was investigated in great detail. Such knowledge not only advances our understanding of the evolution and regulation of sexual dimorphism in insects ...
Ying Yan, Jing Zhao, Jonas Schwirz, Cristina Borghesi, Conghui Liu, Bo Liu, Wanqiang Qian, Fanghao Wan, Marc F. Schetelig   +8 more
wiley   +1 more source

An ancestral secretory apparatus in the protozoan parasite Giardia intestinalis [PDF]

, 2003
The protozoan parasite Giardia intestinalis belongs to one of the earliest diverged eukaryotic lineages. This is also reflected in a simple intracellular organization, as Giardia lacks common subcellular compartments such as mitochondria, peroxisomes ...
Hehl, Adrian B., Knopf, Lea G., Li, Yajie, Marti, Matthias, Müller, Norbert, Regös, Attila, Schraner, Elisabeth M., Wild, Peter   +7 more
core   +1 more source

Functional validation of a white pupae minimal gene construct in Ceratitis capitata (Diptera: Tephritidae)

Insect Science, EarlyView.
An intronless version of the white pupae (wp) gene was engineered to restore the wild type brown puparium color in white pupae phenotype mutants of the Mediterranean fruit fly, Ceratitis capitata. Functionality of the minimal gene version (mini‐wp) was verified in vivo, as one copy of mini‐wp successfully restored the wild type phenotype in homozygous ...
Lucas Henrique Figueiredo Prates, Roswitha A. Aumann, Inga Sievers, Tanja Rehling, Marc F. Schetelig   +4 more
wiley   +1 more source