Results 81 to 90 of about 225,565 (329)

VH-RELATED IDIOTOPES DETECTED BY SITE-DIRECTED MUTAGENESIS [PDF]

, 1992
The function of the CD4 cell surface protein as coreceptor on T helper lymphocytes and as receptor for HIV makes this glycoprotein a prime target for an immune intervention with mAb.
Cheng, Y. H., Rieber, Ernst Peter, Riethmüller, Gert, Weiss, Elisabeth H., Weissenhorn, Winfried   +4 more
core   +1 more source

Characterization and Reuse of Lithium‐ion Battery Cathode Material Recovered Through a Bacterial Process

Advanced Energy Materials, EarlyView.
A sustainable bacteria‐based lithium‐ion battery cathode recycling method is optimized using engineering biology. The resulting biomineralized manganese carbonate species is found to be easily converted into an electrode material, via simple calcination.
Virginia Echavarri‐Bravo, Isolde Marsland, Mai‐Britt V. Jensen, Caroline Kirk, Louise E. Horsfall   +4 more
wiley   +1 more source

Modification of recombinant human epidermal growth factor (rh-EGF) expression vector by site-directed mutagenesis for therapeutic protein production

Indonesian Journal of Biotechnology, 2019
Recombinant human epidermal growth factor (rh-EGF) has high value in therapies for h-EGF deficiency-related diseases. The expression of the h-EGF gene was designed by using the pET21b(+) vector and Escherichia coli BL21(DE3) as the expression host.
Achmad Rodiansyah, Riyona Desvy Pratiwi, Sabighoh Zanjabila, Asrul Muhamad Fuad   +3 more
doaj   +1 more source

Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [PDF]

, 1983
In the presence of O2 and 5 mM dithiothreitol, penta-N-methylpyrrolecarboxamide-EDTA·Fe(II) [P5E·Fe(II)] at 0.5 µ M cleaves pBR322 plasmid DNA (50 µ M in base pairs) on opposite strands to afford discrete DNA fragments as analyzed by agarose gel ...
Dervan, Peter B., Schultz, Peter G.
core  

High-Level Expression of Various Apolipoprotein (a) Isoforms by "Transferrinfection". The Role of Kringle IV Sequences in the Extracellular Association with Low-Density Lipoprotein [PDF]

, 1994
Characterization of the assembly of lipoprotein(a) [Lp(a)] is of fundamental importance to understanding the biosynthesis and metabolism of this atherogenic lipoprotein. Since no established cell lines exist that express Lp(a) or apolipoprotein(a) [apo(a)
Azrolan N., Boerwinkle E., Brunner C., Chiesa G., Chung B. Y., Cotten M., Deutscher S. L., Deutscher S. L., Dieplinger H., Edelberg J. M., Green S., Griffiths G., Guevara J., Jr., Huby T., Ichinose A., Kamboh M. I., Koschinsky M. L., Koschinsky M. L., Koschinsky M. L., Kostner G. M., Kraft H- G., Kraft H. G., Krempler F., Krempler F., Lackner C., Linton M. F., Malgaretti N., McGregor G. R., McLean J. W., Menzel H. J., Phillips M. L., Rader D. J., Sambrook J., Sandholzer C., Scanu A. M., Selden R. F., Sommer A., Stanley P., Trieu V. N., Utermann G., Utermann G., Utermann G., Wagner E., White A. L., Zatloukal K., Zechner R„   +45 more
core   +1 more source

Selection maintains floral color polymorphism in scarlet paintbrush, Castilleja coccinea, reflecting combined ecological factors

American Journal of Botany, EarlyView.
Abstract Premise Evolutionary theory predicts polymorphism should be rare; however, intraspecific variation in floral color is common and can be attributed to genetic drift, plasticity, or variable selection. Examining floral color polymorphism both within contact zones and across a species' range can reveal the mechanisms maintaining this variation ...
Emma Fetterly, Anna S. Braum, Chloe (Eun Sun) Kim, Katherine E. Wenzell, Mary V. Ashley, Laura Steger, Jeremie B. Fant   +6 more
wiley   +1 more source

Modulating Restriction Endonuclease Activities and Specificities Using Neutral Detergents

BioTechniques, 1999
It is well known that type II restriction enzyme activities and specificities can be modulated by altering solution conditions. The addition of co-solvents such as dimethyl sulfoxide (DMSO), alcohols and polyols can promote star activity, which is the ...
Lori H. Conlan, Thomas J. José, Kevin C. Thornton, Cynthia M. Dupureur   +3 more
doaj   +1 more source

Secondary structures in polyoma DNA [PDF]

, 1979
Three reproducible secondary-structure features were observed on single strands of polyoma virus DNA mounted for electron microscopy by the T4 gene 32 protein technique: (i) a hairpin fold-back extending from 92.9 +/- 0.8 to 95.0 +/- 0.7 map units; (ii ...
Davidson, Norman, Manor, Haim, Wu, Madeline   +2 more
core  

A new EcoRI polymorphism for the insulin receptor gene

Nucleic Acids Research, 1989
A 550 bp BamHI-Pst I fragment encompassing bp 1,926-2,476 of the human insulin receptor cDNA was obtained. EcoRI identifies a two allele polymorphism, with bands of 5.8 and 5.5 kb. Co-dominant segregation was demonstrated in one Venezuelan pedigree.
S.C. Elbein, Domenico Accili, Catherine McKeon, Simeon I. Taylor   +3 more
openaire   +4 more sources

Emergence of Staphylococcus aureus Resistance to Antimicrobial Peptides Nisin, NZ2114 and Bacitracin Involves Multiple Phenotypic Changes

Animal Research and One Health, EarlyView.
The AMP‐induced mutant Staphylococcus aureus strain nzR showed increased resistance, five gene mutations, and decreased surface anion levels. AMPs affect the NDH‐2 protein encoded by ndh gene inhibiting the growth of S. aureus. The deletion of the ndh gene promotes the development of AMPs resistance.
Lingyu Xiao, Xingyuan Ma, Ping Tian, Lianbin Li, Zafir Muhammad, Xiaokun Wang, Xuemei Xue, Jinhe Xiao, Liangjun Zheng, F. A. El‐Gohary, Xin Zhao, Junning Pu, Huping Xue   +12 more
wiley   +1 more source