Results 121 to 130 of about 36,103 (144)

Inhibition of platelet aggregation by anthrax edema toxin

Biochemical and Biophysical Research Communications, 2006
Edema toxin is a key virulence determinant in anthrax pathogenesis that causes augmentation of cAMP inside host cells. This exotoxin has been implicated in facilitating bacterial invasion by impairing host defenses. Here, we report for the first time that edema toxin plays an important role in suppression of platelet aggregation; an effect that could ...
Rakesh Bhatnagar, Sheeba Alam
exaly   +3 more sources

Cell entry and cAMP imaging of anthrax edema toxin [PDF]

EMBO Journal, 2006
The entry and enzymatic activity of the anthrax edema factor (EF) in different cell types was studied by monitoring EF-induced changes in intracellular cAMP with biochemical and microscopic methods. cAMP was imaged in live cells, transfected with a fluorescence resonance energy transfer biosensor based on the protein kinase A regulatory and catalytic ...
Cesare Montecucco, Fiorella Tonello, Manuela Zaccolo   +2 more
exaly   +5 more sources

cAMP imaging of cells treated with pertussis toxin, cholera toxin, and anthrax edema toxin

Biochemical and Biophysical Research Communications, 2008
The enzymatic activity of the three most studied bacterial toxins that increase the cytosolic cAMP level: pertussis toxin (PT), cholera toxin (CT), and anthrax edema toxin (ET), was imaged by fluorescence videomicroscopy. Three different cell lines were transfected with a fluorescence resonance energy transfer biosensor based on the PKA regulatory and ...
Cesare Montecucco, Fiorella Tonello, Manuela Zaccolo   +2 more
exaly   +6 more sources

Evidence for adjuvanticity of anthrax edema toxin

Vaccine, 2006
Bacillus anthracis edema factor (EF) is an adenylate cyclase that increases intracellular cAMP concentrations. Since EF is present as a contaminant in the licensed protective antigen(PA)-based vaccines, we investigated its effect on anti-PA humoral immune response in BALB/c mice.
Anne, Quesnel-Hellmann, Aurélie, Cleret, Dominique R, Vidal, Jean-Nicolas, Tournier   +3 more
openaire   +2 more sources

Overview of the role of Shiga toxins in porcine edema disease pathogenesis

Toxicon, 2018
Shiga toxin-producing Escherichia coli (STEC) have been implicated as the cause of enterotoxemias, such as hemolytic uremic syndrome in humans and edema disease (ED) of pigs. Stx1 and Stx2 are the most common types found in association with illness, but only Stx2e is associated with disease in the animal host.
Mariano Enrique Fernández Miyakawa, Natalia A Casanova   +1 more
exaly   +4 more sources

Reproduction of Edema Disease of Swine with Purified Shiga-like Toxin-II Variant

Veterinary Pathology, 1991
Twenty-one weaned Yorkshire-Landrace pigs were injected intravenously with graded doses of purified Shiga-like toxin-II variant (edema disease toxin). In a preliminary study, three pigs (Nos. 1, 2, 3) were injected with 48, 24, and 12 ng, respectively, of SLT-IIv/kg of body weight. Subsequently, three groups (Nos.
B P Wilcock, C L Gyles
exaly   +3 more sources

Modeling the Carbohydrate-Binding Specificity of Pig Edema Toxin

Biochemistry, 1998
The wild-type binding pentamer of Shiga-like toxin IIe (SLT-IIe) binds both the globotriaosylceramide (Gb3) and globotetraosylceramide (Gb4) cell surface glycolipids, whereas the double mutant GT3 (Q65E/K67Q) exhibits a marked preference for Gb3 [Tyrrell, G. J., et al. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 524-528].
M D, Cummings, H, Ling, G D, Armstrong, J L, Brunton, R J, Read   +4 more
openaire   +2 more sources

Lethal and edema toxins of anthrax induce distinct hemodynamic dysfunction

Frontiers in Bioscience, 2007
Fatalities due to anthrax are associated with severe hypotension suggesting that the toxins generated from Bacillus anthracis, lethal toxin (LeTx) and edema toxin (EdTx), have cardiovascular effects. Here, we demonstrate the effects of these toxins and characterize their effects by echocardiography.
Linley E, Watson, Jonathan, Mock, Hind, Lal, Guangrong, Lu, Raymond W, Bourdeau, Wei-Jen, Tang, Stephen H, Leppla, David E, Dostal, Arthur E, Frankel   +8 more
openaire   +2 more sources

Anthrax toxin-induced shock in rats is associated with pulmonary edema and hemorrhage

Microbial Pathogenesis, 2008
Bacillus anthracis infections are frequently associated with severe and often irreversible hypotensive shock despite appropriate antibiotics and aggressive hemodynamic and pulmonary support. Based on the observations that the anthrax secreted proteins-protective antigen (PA), lethal factor (LF), and edema factor (EF) also produce shock and mortality in
Stephen H. Leppla, Nicholas S Duesbery, Wei-Jen Tang   +2 more
exaly   +3 more sources

Murine macrophage transcriptional and functional responses to Bacillus anthracis edema toxin

Microbial Pathogenesis, 2006
Edema toxin (EdTx), which is a combination of edema factor and a binding moiety (protective antigen), is produced by Bacillus anthracis, the etiological agent of anthrax. EdTx is an adenylyl cyclase enzyme that converts adenosine triphosphate to adenosine-3',5'-monophosphate, resulting in interstitial edema seen in anthrax patients.
Jason E, Comer, Cristi L, Galindo, Fan, Zhang, Autumn M, Wenglikowski, Katie L, Bush, Harold R, Garner, Johnny W, Peterson, Ashok K, Chopra   +7 more
openaire   +2 more sources