Results 111 to 120 of about 266,197 (292)

Three-dimensional nonlinear modeling of ELM dynamics with biasing in HL-3 tokamak

Nuclear Fusion
The influence of a biased divertor target system on Edge Localized Mode (ELM) dynamics is investigated for the first time in the HL-3 tokamak, focusing on the impact of bias-driven scrape-off layer (SOL) helical currents on three-dimensional (3D ...
J. Huang, G.Z. Hao, Y. Suzuki, Y.Q. Liu, J.X. Li, Z.J. Li, T.F. Sun, B.T. Cui, H.K. Yin, L. Wang, H.Z. Zhao, X.Q. Ji, W.L. Zhong, Y. Liang, HL-3 Team   +14 more
doaj   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Observation of a stationary double transport barrier in KSTAR

Nuclear Fusion
We have observed a stationary high confinement regime with a double transport barrier (DTB), including both internal and edge transport barriers (ITB and ETB) but without edge-localized modes (ELMs), in KSTAR.
M.W. Lee, S.-H. Hahn, D. Kim, J. Kang, W.H. Ko, J. Jang, W. Lee, C. Sung   +7 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Application of non-integer-n RMP coil current waveform for ELM control in JA DEMO

Nuclear Fusion
We have studied the resonant magnetic perturbation (RMP) coil concept for edge localized mode (ELM) suppression in JA DEMO, a design concept of a steady-state tokamak demonstration reactor, based on the vacuum magnetic model.
S. Sugiyama, N. Aiba, Y. Suzuki, G. Matsunaga, Y. Nakamura, M. Furukawa, A. Ishizawa, Y. Sakamoto   +7 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

EDA H-mode in ASDEX Upgrade: scans of heating power, fueling, and plasma current

Nuclear Fusion
Electron cyclotron resonance heating (ECRH) staircase discharges in strongly shaped plasmas were performed at the full-tungsten ASDEX Upgrade tokamak to investigate the enhanced D _α (EDA) H-mode, a high-confinement regime without edge localized modes ...
L. Gil, T. Pütterich, C. Silva, D. Hachmeister, G.D. Conway, P. David, M. Faitsch, R. Fischer, T. Happel, A. Kallenbach, J. Santos, A. Silva, J. Stober, U. Stroth, E. Viezzer, E. Wolfrum, the ASDEX Upgrade Team, the EUROfusion Tokamak Exploitation Team   +17 more
doaj   +1 more source