Results 81 to 90 of about 1,204,990 (209)

Development and Laboratory Validation of a Field‐Deployable CRISPR‐Cas12a eDNA Assay for Phylogeographic Lineage Detection in Arctic Char (Salvelinus alpinus)

Molecular Ecology Resources, Volume 26, Issue 3, April 2026.
ABSTRACT Environmental DNA (eDNA) tools are increasingly used for biodiversity monitoring, with most existing assays targeting species‐level identification. However, the use of eDNA to resolve intraspecific genetic variation remains rare and methodologically underdeveloped.
Darren J. Walsh, Rosaleen Hynes, Weili Guo, Cliodhna Surgenor, Paulo A. Prodöhl, Anne Parle‐McDermott   +5 more
wiley   +1 more source

Validating metabarcoding-based biodiversity assessments with multi-species occupancy models: A case study using coastal marine eDNA.

PLoS ONE, 2020
Environmental DNA (eDNA) metabarcoding is an increasingly popular method for rapid biodiversity assessment. As with any ecological survey, false negatives can arise during sampling and, if unaccounted for, lead to biased results and potentially ...
Beverly McClenaghan, Zacchaeus G Compson, Mehrdad Hajibabaei   +2 more
doaj   +1 more source

Comparative assessment of eDNA metabarcoding and longline deployments for elasmobranch surveying across a large tropical marine park network

Aquatic conservation
Medium‐ to large‐scale marine protected areas (MPAs) are playing an increasingly important role in global marine biodiversity conservation. A key question is “how do we collect relevant data on biodiversity and population trends to inform their design ...
Katrina M. West, Alastair V. Harry, S. Payet, E. Harvey, Michael J. Travers   +4 more
semanticscholar   +1 more source

Comparative Multi‐Marker Environmental DNA Metabarcoding of Marine Metazoan Communities: Water vs. Sediment

Molecular Ecology Resources, Volume 26, Issue 3, April 2026.
ABSTRACT This study investigates the metazoan biodiversity in the Southern Adriatic Sea using environmental DNA (eDNA) metabarcoding. Sediment and adjacent water samples were collected from three sites (one pristine, two impacted by human activities) at three distances from the coast across two seasons.
Alice Tagliabue, Giulia Furfaro, Alberto Pallavicini, Francesco Martino, Lorenzo Zane, Eleonora Sattin, Giorgio Valle, Stefano Piraino, Xavier Turon   +8 more
wiley   +1 more source

Validation of fish environmental RNA metabarcoding analysis for ecological surveys by additional traditional field surveys in the Naka River

Frontiers in Ecology and Evolution
Metabarcoding analysis using fish environmental RNA (eRNA) has demonstrated higher performance compared to that using fish environmental DNA (eDNA) in the Naka River in Japan.
Kaede Miyata, Yuta Kusakabe, Yasuaki Inoue, Masayuki Yamane, Hiroshi Honda   +4 more
doaj   +1 more source

Finding the perfect pairs: A matchmaking of plant markers and primers for multi-marker eDNA metabarcoding.

Molecular Ecology Resources
As the scope of plant eDNA metabarcoding diversifies, so do the primers, markers and methods. A wealth of primers exists today, but their comparative evaluation is lacking behind.
Armando Espinosa Prieto, L. Hardion, Nicolas Debortoli, J. Beisel   +3 more
semanticscholar   +1 more source

Disentangled Assembly Graphs Reveal Hidden Eukaryotic Diversity in eDNA Metagenomic Data

Molecular Ecology Resources, Volume 26, Issue 3, April 2026.
ABSTRACT Genome assembly graphs contain valuable yet frequently overlooked information that can enhance assembly completeness by revealing contig connectivity. Here, we demonstrate how leveraging these information‐rich structures enables the discovery of hidden microeukaryotic diversity in environmental DNA shotgun metagenomic datasets.
Manon Mireille Geerts, Manuel Curto, Andrew J. Alverson, Jeffery Stone, Hugo F. Gante   +4 more
wiley   +1 more source

Persistence of marine fish environmental DNA and the influence of sunlight.

PLoS ONE, 2017
Harnessing information encoded in environmental DNA (eDNA) in marine waters has the potential to revolutionize marine biomonitoring. Whether using organism-specific quantitative PCR assays or metabarcoding in conjunction with amplicon sequencing ...
Elizabeth A Andruszkiewicz, Lauren M Sassoubre, Alexandria B Boehm   +2 more
doaj   +1 more source

Fungal ecology in the age of 'omics

New Phytologist, Volume 250, Issue 2, Page 822-833, April 2026.
Summary The advancement of technology in recent decades has given us an unprecedented ability to observe the natural world. With modern sequencing and bioinformatics technologies, we can obtain more information about the microscopic world, and its interactions with the macroscopic world, than ever before.
Brontë R. Shelton, Joana Larrere, Diego Yusta Belsham, Marina Omacini, Andrés Argüelles‐Moyao, Erika Buscardo, Danielle Karla Alves da Silva, Xiaoyan Zhao, Naoto Nakamura, Rodolfo Ángeles‐Argáiz, Claudia Paz, Noemí Matías‐Ferrer, Miranda Mistaya Hart   +12 more
wiley   +1 more source

High‐Throughput DNA Sequencing Reveals Gastric Content Composition and Inter‐Specific Variation in Pampus Fishes

Ecology and Evolution, Volume 16, Issue 3, March 2026.
In this study, we employed high‐throughput sequencing of environmental DNA (eDNA) to conduct an analysis of the gastric contents composition and relative abundance from samples of P. argenteus and P. punctatissimus collected at the Jimiya Fish Pier in Qingdao, Shandong Province, and P. cinereus collected at the Qinglan Port in Wenchang, Hainan Province.
Wang Weiji, Liu Chengbin, Lv Ding, Hu Yulong, Lv Guanzheng, Shan Xiujuan   +5 more
wiley   +1 more source