Results 181 to 190 of about 28,276 (232)

Variability of calcium binding to EF-hand motifs probed by electrospray ionization mass spectrometry

Journal of the American Society for Mass Spectrometry, 2001
The modulation of calcium binding by the EF-hand motifs present in a calmodulin (CAM) homologue, a calcium binding protein (CaBP) from Entamoeba histolytica by three external parameters-pH, ligand coordinator EGTA, and fragmentor voltage was investigated by mass spectrometry.
A. Moorthy, S. Singh, B. Gopal, A. Surolia, M. Murthy   +4 more
semanticscholar   +3 more sources

Some of the next articles are maybe not open access.

Related searches:

Functional analysis of calcium-binding EF-hand motifs of visinin-like protein-1.

Biochemical and Biophysical Research Communications, 2002
Visinin-like protein-1 (VILIP-1), a myristoylated calcium sensor protein with three EF-hand motifs, modulates adenylyl cyclase activity. It translocates to membranes when a postulated "calcium-myristoyl switch" is triggered by calcium-binding to expose its sequestered myristoyl moiety.
Lin-Li Lin, K. Braunewell, E. Gundelfinger, R. Anand   +3 more
semanticscholar   +3 more sources

A reporter system that discriminates EF‐hand‐sensor motifs from signal‐modulators at the single‐motif level [PDF]

FEBS Letters, 2012
The T‐protein is a single‐polypeptide bi‐functional enzyme composed of a chorismate mutase domain fused to a prephenate dehydrogenase domain (TyrA). We replaced the chorismate mutase domain with canonical or pseudo‐Ca2+‐binding motifs (EF‐hand).
J. Osuna, H. Flores, P. Gaytán
semanticscholar   +3 more sources

Chimeric HTH motifs based on EF-hands

JBIC Journal of Biological Inorganic Chemistry, 2001
The design of a new peptide construct from two structurally equivalent basis motifs is reported. A chimera was designed from the helical regions of a helix-turn-helix (HTH) domain, incorporating the consensus EF-hand Ca-binding loop at the turn. Two 33-residue peptides were constructed: one (P3, designed) includes the 12-residue consensus EF-hand loop,
Y, Kim, J T, Welch, K M, Lindstrom, S J, Franklin   +3 more
openaire   +2 more sources

Identification of mouse itih-4 encoding a glycoprotein with two EF-hand motifs from early embryonic liver.

Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1998
An essential feature of cell differentiation is the specificity of signal transduction events from extracellular cues, which are considered to be conferred by scaffold, anchoring and adaptor proteins. Our aim was to identify important scaffolding proteins required for liver development.
T. Cai, P. Yu, S. Monga, B. Mishra, L. Mishra   +4 more
semanticscholar   +3 more sources

The regulatory role of EF-hand motifs of pig 80K diacylglycerol kinase as assessed using truncation and deletion mutants.

Biochemical and Biophysical Research Communications, 1991
To elucidate the regulatory function of EF-hand motifs of pig 80K diacylglycerol (DG) kinase, we constructed and expressed several truncation and deletion mutants of the enzyme in E. coli or COS-7 cells. The bacterially expressed EF-hand region could bind Ca2+ and was suggested to undergo conformational change like calmodulin.
F. Sakane, S. Imai, Keiko Yamada, H. Kanoh   +3 more
semanticscholar   +3 more sources

Conformational changes and calcium binding by calretinin and its recombinant fragments containing different sets of EF hand motifs.

Biochemistry, 1995
Four recombinant fragments, representing different sets of EF-hand motifs of rat calretinin (CR) (I-II, I-III, III-VI, IV-VI), were prepared, and their Ca2+ -induced conformational changes were compared with those of full-length recombinant CR. All fragments were able to bind calcium ions as shown by 45Ca2+ overlay method on nitrocellulose and ...
J. Kuźnicki, K. Strauss, D. Jacobowitz
semanticscholar   +3 more sources

Sequence of a human cDNA encoding Cab45, a Ca2+-binding protein with six EF-hand motifs.

DNA Sequence, 1997
We report the sequence of a human cDNA encoding a deduced 362 amino acid protein with six EF-hand calcium-binding motifs. The protein is a likely human counterpart of the Cab45 protein recently identified in the 3T3-L1 mouse adipocyte cell line [Scherer et al. (1996), J. Cell Biol. 133, 257-268], displaying 87% aa and 83% nt identity with this sequence.
T. Koivu, S. Laitinen, K. Riento, V. Olkkonen   +3 more
semanticscholar   +3 more sources

Crystal structure of calcium binding protein‐1 from Entamoeba histolytica: A novel arrangement of EF hand motifs

Proteins: Structure, Function, and Bioinformatics, 2007
AbstractCalcium plays a pivotal role in the pathogenesis of amoebiasis, a major disease caused by Entamoeba histolytica. Several EF‐hand containing calcium‐binding proteins (CaBPs) have been identified from E. histolytica. Even though these proteins have very high sequence similarity, they bind to different target proteins in a Ca2+ dependent manner ...
Shivesh Kumar, Narendra Padhan, N. Alam, S. Gourinath   +3 more
semanticscholar   +3 more sources

EF hand‐like motif mutations of Nav1.4 C‐terminus cause myotonic syndrome by impairing fast inactivation

Muscle & Nerve, 2020
AbstractIntroductionMutations of the voltage‐gated sodium channel gene (SCN4A), which encodes Nav1.4, cause nondystrophic myotonia that occasionally is associated with severe apnea and laryngospasm. There are case reports of nondystrophic myotonia due to mutations in the C‐terminal tail (CTerm) of Nav1.4, but the functional analysis is scarce.MethodsWe
Riho, Horie, Tomoya, Kubota, Jinsoo, Koh, Rieko, Tanaka, Yuichiro, Nakamura, Ryogen, Sasaki, Hidefumi, Ito, Masanori P, Takahashi   +7 more
openaire   +2 more sources