Results 111 to 120 of about 13,443,244 (418)

High-efficiency transformation of mammalian cells by plasmid DNA.

Molecular and Cellular Biology, 1987
We describe a simple calcium phosphate transfection protocol and neo marker vectors that achieve highly efficient transformation of mammalian cells. In this protocol, the calcium phosphate-DNA complex is formed gradually in the medium during incubation ...
Claudia A. Chen, H. Okayama
semanticscholar   +1 more source

A cellular system to study responses to a collision between the transcription complex and a protein‐bound nick in the DNA template

FEBS Letters, EarlyView.
We present the cellular transcription‐coupled Flp‐nick system allowing the introduction of a Top1‐mimicking cleavage complex (Flpcc) at a Flp recognition target site within a controllable LacZ gene. LacZ transcription leads to the collision of RNA polymerase II (RNAPII) with Flpcc, and this causes RNAPII stalling, ubiquitination, and degradation.
Petra Herring, Morten Roedgaard, Camilla Myrup Holst, Helene Christensen, Birgitta R. Knudsen, Lotte Bjergbaek, Anni Hangaard Andersen   +6 more
wiley   +1 more source

Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data

Nucleic Acids Research, 2009
Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA transcripts, most analyses of qPCR data are still delegated to the software that comes with the qPCR apparatus. This is especially true for the handling of the fluorescence
J. Ruijter, Christian Ramakers, W. Hoogaars, Y. Karlen, O. Bakker, M. Hoff, A. Moorman   +6 more
semanticscholar   +1 more source

Wind Powering America Initiative: The Wind and Water Power Program (WWPP)

, 2011
The U.S. Department of Energy\u27s Wind Powering America initiative engages in technology market acceptance, barrier reduction, and technology deployment support activities.
Energy Efficiency & Renewable Energy Information Center
core  

Efficiency gains and mergers [PDF]

, 2008
In the theoretical literature, strong arguments have been provided in support of the efficiency defense in antitrust merger policy. One of the most often cited results is due to Williamson (1968) that shows how relatively small reduction in cost could ...
De Feo, Giuseppe
core   +2 more sources

Interaction vesicles as emerging mediators of host‐pathogen molecular crosstalk and their implications for infection dynamics

FEBS Letters, EarlyView.
Interaction extracellular vesicles (iEVs) are hybrid vesicles formed through host‐pathogen communication. They facilitate immune evasion, transfer pathogens' molecules, increase host cell uptake, and enhance virulence. This Perspective article illustrates the multifunctional roles of iEVs and highlights their emerging relevance in infection dynamics ...
Bruna Sabatke, Izadora Volpato Rossi, Marcel I. Ramirez   +2 more
wiley   +1 more source

Stiffness Modeling of Tooth Interior Fatigue and Tooth Flank Fracture and Their Fault Characteristics in Electromechanical Coupling Systems

Shock and Vibration
Tooth flank fracture (TFF) and tooth interior fatigue fracture (TIFF) usually occur on case-harden gears in electromechanical coupling systems, both starting from the core caused by metal fatigue. The cracks propagate rapidly compared with the total life
Lin Han, Dianrui Wang, Muhammad Anwar Jan, Fuchun Yang   +3 more
doaj   +1 more source

On the mobility and efficiency of mechanical systems [PDF]

arXiv, 2005
The definition of a mobilized system and its efficiency are introduced. The existence of an optimal (maximally efficient) system is proved by an application of Young measures and compensated compactness.
arxiv  

STAT3 expression is reduced in cardiac pericytes in HFpEF and its loss reduces cellular adhesion and induces pericyte senescence

FEBS Letters, EarlyView.
Heart failure with preserved ejection fraction (HFpEF) accounts for half of the heart failure cases. It is characterised by microvascular dysfunction, associated with reduced pericyte coverage and diminished STAT3 expression in pericytes. Loss of STAT3 impairs pericyte adhesion, promotes senescence, and activates a pro‐fibrotic gene program.
Leah Rebecca Vanicek, Ariane Fischer, Mariano Ruz Jurado, Anita Tamiato, Tara Procida‐Kowalski, Jochen Wilhelm, Dennis Hecker, Maximilian Merten, Felicitas Escher, Badder Kattih, Valentina Puntmann, David John, Marcel H. Schulz, Eike Nagel, Stefanie Dimmeler, Guillermo Luxán   +15 more
wiley   +1 more source

Structure and complexity of ex post efficient random assignments [PDF]

arXiv, 2014
In the random assignment problem, objects are randomly assigned to agents keeping in view the agents' preferences over objects. A random assignment specifies the probability of an agent getting an object. We examine the structural and computational aspects of ex post efficiency of random assignments.
arxiv