Results 121 to 130 of about 800 (143)

Antibodies to Epizootic Hemorrhagic Disease Virus (EHDV) in Farmed and Wild Florida White-Tailed Deer (Odocoileus virginianus)

Journal of Wildlife Diseases, 2020
The transmission of multiple serotypes of epizootic hemorrhagic disease virus (EHDV) between farmed and free-ranging wildlife is of interest to livestock industries and natural resource agencies. We compared the seroprevalence of EHDV-1, -2, and -6 in wild and farmed white-tailed deer (Odocoileus virginianus) herds in Florida, US.
Jeremy P Orange, Jason K Blackburn, Samantha M Wisely   +2 more
exaly   +3 more sources

Contamination of Genetically Engineered CHO-cells by Epizootic Haemorrhagic Disease Virus (EHDV)

Biologicals, 1993
The characterization of a contaminating virus which was detected in genetically-engineered Chinese hamster ovary (CHO) cells during the production of biologicals is described in the present paper. Under electron microscopy, the contaminating virus had a morphology resembling that of an orbivirus.
H, Rabenau, V, Ohlinger, J, Anderson, B, Selb, J, Cinatl, W, Wolf, J, Frost, P, Mellor, H W, Doerr   +8 more
openaire   +2 more sources

Detection of a novel reassortant epizootic hemorrhagic disease virus serotype 6 in cattle in Trinidad, West Indies, containing nine RNA segments derived from exotic EHDV strains with an Australian origin

Infection, Genetics and Evolution, 2019
Epizootic hemorrhagic disease virus (EHDV) is a Culicoides-transmitted orbivirus that infects domestic and wild ruminants in many parts of the world. Of the eight proposed serotypes, only EHDV-1, 2 and 6 have been reported to be present in the Americas.
Paulina Rajko-Nenow, John Flannery, Chris Oura   +2 more
exaly   +3 more sources

Detection of EHDV Antibodies Using Enzyme-Linked Immunosorbent Assay (ELISA)

Methods in Molecular Biology
Enzyme-linked immunosorbent assay (ELISA) is a relatively inexpensive, rapid, and high-throughput diagnostic tool to detect antibodies raised against epizootic hemorrhagic disease virus (EHDV) in ruminant serum. While the presence of EHDV antibodies only confirms prior exposure to the virus, it does not conclusively determine infection status.
Carrie Batten, Batten Carrie
exaly   +3 more sources

RNA segment 9 exists as a duplex concatemer in an Australian strain of epizootic haemorrhagic disease virus (EHDV): Genetic analysis and evidence for the presence of concatemers as a normal feature of orbivirus replication

Virology, 2011
This paper reports a concatemeric RNA in a strain of epizootic haemorrhagic disease virus (EHDV) serotype 5. Sequencing showed that the concatemeric RNA contains two identical full-length copies of genome segment 9, arranged in series, which has apparently replaced the monomeric form of the segment.
Kyriaki Nomikou, G Sutton, Houssam Attoui   +2 more
exaly   +6 more sources

65 Inactivation Kinetics of Epizootic Hemorrhagic Disease Virus (EHDV) By Ingredients Contained in Expect Healthy Deer Technology®.

Journal of Animal Science, 2018
A M Gaines, Hamada A Aboubakr, Gaines A M   +2 more
exaly   +2 more sources

Application of molecular biological techniques for detection of epizootic hemorrhagic disease virus (EHDV-318) recovered from a sentinel calf in central Sudan

Veterinary Microbiology, 1996
Epizootic hemorrhagic disease virus (EHDV), isolate 318 (EHDV-318), an untyped virus recovered from a sentinel calf herd at the Khartoum University farm in central Sudan, was characterized using molecular biological techniques. With dot blot hybridization technique, a cDNA probe derived from genome segment 6 of EHDV-2 (Alberta strain) hybridized with ...
H W Ghalib, B I Osburn, Imadeldin E Aradaib   +2 more
exaly   +3 more sources

Confocal Microscopy of Fluorescently Labeled EHDV-Infected Cell Cultures

The confocal laser scanning microscope allows the visualization of intracellular structures in greater detail than a widefield fluorescence microscope. Immunofluorescence (IF) techniques make use of the inherent ability of antibodies to bind to specific epitopes of specific proteins.
Joanna, Wells, Amanda, Corla, Philippa C, Hawes   +2 more
openaire   +2 more sources

The evolution of two homologues of the core protein VP6 of epizootic haemorrhagic disease virus (EHDV), which correspond to the geographical origin of the virus

Virus Genes, 2009
Epizootic haemorrhagic disease virus is a 10-segmented, double-stranded RNA virus. When these ten segments of dsRNA are run on 1% agarose, eastern (Australia, Japan) and western (North America, Africa, Middle-East) strains of the virus can be separated phenotypically based on the migration of genome segments 7-9.
Anthony, S, Darpel, K, Maan, S, Sutton, G, Attoui, Houssam, Mertens, P   +5 more
openaire   +4 more sources

Competitive enzyme-linked immunosorbent assay using baculovirus-expressed VP7 for detection of epizootic haemorrhagic disease virus (EHDV) antibodies

Journal of Virological Methods, 2017
Epizootic haemorrhagic disease (EHD) is a vector-borne infectious viral disease of domestic and wild ruminants. EHD could spread from infected northern African countries in free territories like the EU; therefore, the availability of diagnostic assays would represent key components for adequate surveillance and control programs. In this study, the gene
FORZAN, MARIO, PIZZURRO, FEDERICA, Zaccaria, Guendalina, MAZZEI, MAURIZIO, Spedicato, Massimo, Carmine, Irene, Salini, Romolo, TOLARI, FRANCESCO, CERRI, DOMENICO, Savini, Giovanni, Lorusso, Alessio   +10 more
openaire   +2 more sources