Results 101 to 110 of about 15,232 (136)

Regulation of protein synthesis by heme-regulated eIF-2α kinase

Trends in Biochemical Sciences, 1995
Protein synthesis is regulated by the phosphorylation of the alpha-subunit of eukaryotic initiation factor 2 (eIF-2 alpha) in a variety of cells. At present, there are two distinct mammalian eIF-2 alpha kinases that have been cloned, the double-stranded-RNA-dependent eIF-2 alpha kinase (PKR) and the heme-regulated eIF-2 alpha kinase (HRI).
J J, Chen, I M, London
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Purification of a novel eIF-2α protein kinase from calf brain

Biochemical and Biophysical Research Communications, 1990
A new eukaryotic initiation factor 2 kinase has been purified for the first time from calf brain cytosol. The purification of a nonabundant novel protein kinase activity, designated as PKI, that phosphorylates the alpha subunit of eukaryotic initiation factor 2 is described.
A, Alcázar, E, Méndez, M E, Martín, M, Salinas   +3 more
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Interdomain interactions regulate the activation of the heme-regulated eIF2α kinase

Biochimica et Biophysica Acta (BBA) - General Subjects, 2005
The heme-regulated inhibitor of protein synthesis (HRI) regulates translation through the phosphorylation of the alpha-subunit of eukaryotic initiation factor-2 (eIF 2). While HRI is best known for its activation in response to heme-deficiency, we recently showed that the binding of NO and CO to the N-terminal heme-binding domain (NT-HBD) of HRI ...
Bo-Geon, Yun, Jessica A B, Matts, Robert L, Matts   +2 more
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Regulation of the interferon-inducible eIF-2α protein kinase by small RNAs

Biochimie, 1994
This review describes the structure and function of the double-stranded RNA-dependent protein kinase (PKR) and its interaction with RNA activators and inhibitors. The abilities of small virally-encoded RNAs such as VAI RNA of adenovirus, the Epstein-Barr virus encoded (EBER) RNAs and the Tat-responsive region RNA of HIV-1 to bind to and regulate PKR ...
M J, Clemens, K G, Laing, I W, Jeffrey, A, Schofield, T V, Sharp, A, Elia, V, Matys, M C, James, V J, Tilleray   +8 more
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Denatured proteins inhibit translation in hemin-supplemented rabbit reticulocyte lysate by inducing the activation of the heme-regulated eIF-2.alpha. kinase

Biochemistry, 1993
The heme-regulated inhibitor (HRI) of protein synthesis becomes activated in rabbit reticulocyte lysates in response to a variety of conditions including heme-deficiency, addition of oxidants, and heat shock. Activated HRI inhibits translation by catalyzing the phosphorylation of the alpha-subunit of eukaryotic initiation factor eIF-2.
R L, Matts, R, Hurst, Z, Xu
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Isolation of a translational inhibitor from wheat germ with protein kinase activity that phosphorylates initiation factor eIF-2

Biochemical and Biophysical Research Communications, 1980
Abstract A translational inhibitor (WGI) has been partially purified from wheat germ extracts. WGI inhibits protein synthesis in rabbit reticulocyte lysates with inhibition kinetics that are similar to those observed in heme-deficiency or by the addition of purified heme-regulated translational inhibitor (HRI).
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Molecular Cloning and Sequencing of the Human Heme-regulated Eukaryotic Initiation Factor 2 Alpha (eIF-2 Alpha) Kinase from Bone Marrow Culture

DNA Sequence, 2002
The cDNA encoding human heme-regulated eukaryotic initiation factor-2 alpha (eIF-2 alpha) kinase was cloned from a human bone marrow culture. Its deduced amino acid sequence comprised of 629 amino acids with a calculated molecular weight of 71,031 Da.
Takeshi, Omasa, Yi-Guang, Chen, Athanasios, Mantalaris, Ying-Chuech, Tsai, J H David, Wu   +4 more
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The 90-kilodalton peptide of the heme-regulated eIF-2.alpha. kinase has sequence homology with the 90-kilodalton heat shock protein

Biochemistry, 1987
Highly purified preparations of the heme-controlled eIF-2 alpha (eukaryotic peptide initiation factor 2 alpha subunit) kinase of rabbit reticulocytes contain an abundant 90-kilodalton (kDa) peptide that is immunologically cross-reactive with spectrin and that modulates the activity of the enzyme [Kudlicki, W., Fullilove, S., Read, R., Kramer, G ...
D W, Rose, R E, Wettenhall, W, Kudlicki, G, Kramer, B, Hardesty   +4 more
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elF-2 kinases: regulators of general and gene-specific translation initiation

Trends in Biochemical Sciences, 1994
Phosphorylation of eukaryotic initiation factor-2 (eIF-2) is an important mechanism regulating general translation initiation. Two mammalian eIF-2 kinases, the double-stranded-RNA-dependent kinase (PKR) and heme-regulated inhibitor kinase (HRI), have been characterized by sequencing, revealing shared sequence and structural features distinct from other
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Regulation of heme-regulated eIF-2α kinase and its expression in erythroid cells

Biochimie, 1994
In this article we focus first on the molecular mechanisms controlling the activity of the heme-regulated translational inhibitor, HRI, in erythroid cells. Then we discuss the tissue-specific expression of HRI. The experimental evidence obtained to date indicates that the major physiological role of HRI is in adjusting the synthesis of globin to the ...
J J, Chen, J S, Crosby, I M, London
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