Results 311 to 320 of about 111,880 (336)
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Analytical Biochemistry, 1973
Abstract An assay for insoluble elastin is described. The procedure involves isolation of elastin by 5 m guanidine and autoclaving. The residue is solubilized with elastase and the protein released estimated colorimetrically. This assay method is sensitive in the range: 50–300 μg.
Yvonne Naum +3 more
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Abstract An assay for insoluble elastin is described. The procedure involves isolation of elastin by 5 m guanidine and autoclaving. The residue is solubilized with elastase and the protein released estimated colorimetrically. This assay method is sensitive in the range: 50–300 μg.
Yvonne Naum +3 more
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Pulmonary Pharmacology & Therapeutics, 2012
Extracellular matrix is generally increased in asthma, causing thickening of the airways which may either increase or decrease airway responsiveness, depending on the mechanical requirements of the deposited matrix. However, in vitro studies have shown that the altered extracellular matrix produced by asthmatic airway smooth muscle cells is able to ...
Janette K. Burgess +2 more
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Extracellular matrix is generally increased in asthma, causing thickening of the airways which may either increase or decrease airway responsiveness, depending on the mechanical requirements of the deposited matrix. However, in vitro studies have shown that the altered extracellular matrix produced by asthmatic airway smooth muscle cells is able to ...
Janette K. Burgess +2 more
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Stain Technology, 1963
Conditions relevant to the acceptance of acid dyes by the elastin of the aorta are as follows: (a) Fixation. Cold alcohol is poor, formol-sublimate, hest; others are intermediate. (h) Acid dyes used. Bromphenol blue is best, light green is poor, acid fuchsin is unstable in alkaline solution.
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Conditions relevant to the acceptance of acid dyes by the elastin of the aorta are as follows: (a) Fixation. Cold alcohol is poor, formol-sublimate, hest; others are intermediate. (h) Acid dyes used. Bromphenol blue is best, light green is poor, acid fuchsin is unstable in alkaline solution.
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Nature, 1967
PARTRIDGE et al.1 observed that hydrolysis of pure insoluble elastin from bovine ligamentum nuchae by oxalic acid gave rise to soluble elastin consisting of two fractions: α-elastin with a mean molecular weight of 60,000–84,000; and β-elastin with a mean molecular weight of 5,000.
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PARTRIDGE et al.1 observed that hydrolysis of pure insoluble elastin from bovine ligamentum nuchae by oxalic acid gave rise to soluble elastin consisting of two fractions: α-elastin with a mean molecular weight of 60,000–84,000; and β-elastin with a mean molecular weight of 5,000.
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1987
Publisher Summary This chapter describes different methods for the preparation of polyclonal and monoclonal antibodies to insoluble or solubilized elastin and will detail their use in a variety of immunological procedures. Antisera have been prepared to insoluble and solubilized elastin using a variety of antigen preparations and animal hosts.
Robert P. Mecham, David S. Wrenn
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Publisher Summary This chapter describes different methods for the preparation of polyclonal and monoclonal antibodies to insoluble or solubilized elastin and will detail their use in a variety of immunological procedures. Antisera have been prepared to insoluble and solubilized elastin using a variety of antigen preparations and animal hosts.
Robert P. Mecham, David S. Wrenn
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Collagen and Elastin in Scleroderma
Connective Tissue Research, 1974The amino acid compositions of insoluble collagen and elastin purified from skin biopsies of normal subjects and scleroderma patients were conlpared. Collagen from dermis-subcutaneous tissue from the patients shows a significant increase of 3-OH proline and a slight increase in 4-OH proline and OH-lysine.The greater hydroxylation in collagen of ...
GIRO MG +2 more
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Matrix Biology, 2019
Elastic fibers are an essential component of the extracellular matrix where they provide structural integrity and elastic recoil in a number of important tissues. A major constituent of these fibers is elastin, an insoluble metabolically stable polymer formed via extensive crosslinking of the monomeric precursor tropoelastin. Research over the past few
Howard, Vindin +2 more
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Elastic fibers are an essential component of the extracellular matrix where they provide structural integrity and elastic recoil in a number of important tissues. A major constituent of these fibers is elastin, an insoluble metabolically stable polymer formed via extensive crosslinking of the monomeric precursor tropoelastin. Research over the past few
Howard, Vindin +2 more
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Elastin in Vascular Grafts [PDF]
, 2019 The clinical demand for a superior vascular graft is rising due to the increase in cardiovascular disease with an aging population. Despite decades of research, clinically translatable solutions remain limited. Recent progress in vascular graft engineering has highlighted the significance of biological integration for the success of implanted grafts ...
Anthony S. Weiss +3 more
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2021
Connective tissues provide multicellular animals with the basic fabric for stabilizing the three-dimensional organization of cells. The loss or dysfunction of elastic tissue can have severe pathological consequences. The elastic fiber has been recognized as a histological entity for at least a century.
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Connective tissues provide multicellular animals with the basic fabric for stabilizing the three-dimensional organization of cells. The loss or dysfunction of elastic tissue can have severe pathological consequences. The elastic fiber has been recognized as a histological entity for at least a century.
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Evolution of Elastin Structure [PDF]
, 1977 The aortae of a number of vertebrates and invertebrates were tested for the presence of elastin to determine when this protein first appears phylogenetically. Using several criteria, including amino acid composition, presence of desmosines, and histological characteristics, we found that all representative vertebrate groups have elastin except the ...
E. H. Sage, W. R. Gray
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