Results 41 to 50 of about 1,277,194 (266)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Analysis of the influence of changing hydraulic load on the mechanical characteristics of a centrifugal pump with an asynchronous electric drive

Технічна інженерія
The article analyzes the influence of changing hydraulic load on the mechanical characteristics of a centrifugal pump with an asynchronous electric drive.
Yu.O. Podchashynskyi, Ya.V. Mahaletskyi
doaj   +1 more source

Myogenic Fusogen‐Engineered Lipid Nanoparticles Enhance mRNA Delivery in Skeletal Muscle

Advanced Functional Materials, EarlyView.
This study reports a biomimetic strategy of engineering full‐length Myomaker, a muscle‐specific fusogen, into lipid nanoparticles (LNPs) to harness the native myoblast fusion capability for skeletal muscle mRNA delivery. The resulting Mymk‐LNPs enhance transfection in differentiating myocytes and enable Cre‐mediated reporter activation in injured ...
Fangyu Zhang, Zhisheng Xu, Yaou Duan, Yehui Sun, Shiying Wu, Tao Long, Mateusz Durbacz, Li Zhang, Yufen Xiao, Yu‐Chung Pien, Erick D. Guerrero, Zeru Tian, Xu Wang, Pratima Basak, Sumanta Chatterjee, Ning Liu, Xiaochun Li, Eric N. Olson, Daniel J. Siegwart   +18 more
wiley   +1 more source

Additively Manufactured Geometry‐Reconfigurable Soft Neural Probes With Monolithic Multifunctionality

Advanced Functional Materials, EarlyView.
A geometry‐reconfigured additive manufacturing strategy converts planar printed soft materials into soft compact neural probes with integrated hydrogel electrodes and microfluidic channels. The resulting NeuroMAP platform enables scalable multichannel recording, localized chemical delivery, stable in vivo electrophysiological recording, and reduced ...
Hyunjin Lee, Xinyi Wang, Sirui Li, Jihyang Park, Hyunseok Lee, Jianting Yao, Kedar Narayan, Yuqi Wang, Jiashu Ren, Yueqi Deng, Salahuddin Ahmed, Denver I. Greenawalt, Archana Pandiyan, Loganathan Veeramuthu, Chi‐Ching Kuo, Tao Zhou   +15 more
wiley   +1 more source

Influence of Operational Parameters on Centrifugal Pump Coupling Loads: A Case Study

Journal of Mechanical Engineering
An analysis of the operating dynamics of a centrifugal pump driven by an asynchronous electric motor was conducted using a two-mass torsional model incorporating a reduction element as the pump’s flexible coupling.
Protsenko Vladyslav, Ondruška Juraj, Rusanov Sergiy, Protasov Roman, Danko Ján, Kliuev Oleg, Voitovych Olha   +6 more
doaj   +1 more source

Typical Case Analysis of the Building-type CCHP System

南方能源建设, 2021
[Introduction] The paper aims to obtain optimal configuration and operation strategy of the distributed energy system which is based on the calculation of the annual typical daily cooling, heating and electric load demand of various buildings.
CHEN Hui, LIU Ying, ZHAO Longsheng
doaj   +1 more source

Utilizing the Metastability of Spidroins as a Strategy for Improving Mechanical Properties of Biomimetic Spider Silk Fibers

Advanced Functional Materials, EarlyView.
Phase transitions are central for the assembly of recombinant silk proteins into fibers. Soluble protein can transition into condensed states such as condensates and gels. These states are metastable and evolve in a time‐dependent way. Metastability depends on the protein sequence, and this relationship can be used to enhance the mechanical properties ...
Fred‐Eric Sammalisto, Topias Lankinen, Celine Tallroth, Rafael A. Lara, Matilda Backholm, Markus B. Linder   +5 more
wiley   +1 more source

Modeling of Hydraulic Load of Electric Drive in Electrical Complex of Pumping Station

Energy Engineering and Control Systems, 2018
The paper analyses the contemporary state of the electric drive hydraulic load modelling in the pumping stations’ electrotechnical complex applications. It was found that in the vast majority of cases, mathematical models do not allow taking into account
Vladyslav Lysiak, Mykhailo Oliinyk, Yurii Shelekh   +2 more
doaj   +1 more source

Near‐Infrared Light‐Activated Polydopamine Nanoparticles Conjugated With Transferrin Peptides for Enhanced Brain Accumulation

Advanced Healthcare Materials, EarlyView.
This study demonstrates that Tf‐PDA NPs combine a receptor‐mediated targeting with mild photothermal activation to enhance nanoparticle accumulation in the brain. NIR‐activated Tf‐PDA NPs increase BBB permeability to NPs in vitro and in vivo without inducing cytotoxicity or adverse immune responses.
Rafaela Ferrão, Susana Simões, Paulo Rodrigues‐Santos, Vitor Francisco, Akhilesh Rai, Lino Ferreira   +5 more
wiley   +1 more source

Increasing the Time between Failures of Electric Submersible Pumps for Oil Production with High Content of Mechanical Impurities

Applied Sciences, 2021
The main principles of hydroabrasive wear of parts of the pumping stages of well’s electric submersible pumps are considered in this article. The concentration, grain-size distribution and shape of solid particles have the greatest impact on the abrasion
Dmitriy Shishlyannikov, Valeriy Zverev, Anna Ivanchenko, Ivan Zvonarev   +3 more
doaj   +1 more source