Results 51 to 60 of about 663,813 (317)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Modeling Electricity Auctions [PDF]

, 2002
The recent debates over discriminatory versus uniform-price auctions in the U.K. and elsewhere have revealed an incomplete understanding of the limitations of some popular auction models when applied to real-world electricity markets.
Fabra, Natalia, Harbord, David, Fehr, Nils-Henrik Von der   +2 more
core   +3 more sources

Evaluating Preparedness and Overcoming Challenges in Electricity Trading: An In-Depth Analysis Using the Analytic Hierarchy Process and a Case Study Exploration

Electricity
The economy of South Asia is experiencing growth, yet it faces constraints due to heavy reliance on fossil fuels and frequent power outages. Access to diverse energy sources, particularly electricity, is crucial for sustaining this growth.
Suraj Regmi, Abhinav Rayamajhi, Ramhari Poudyal, Sanjeev Adhikari   +3 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

The Implementation and Evaluation of Virtualized Protection Intelligent Electronic Devices into a Virtual Substation

Electricity
This paper presents an investigation into the virtualization of substation protection IED functions using a sophisticated co-simulation environment that integrates virtual intelligent electronic devices (vIEDs) with a real-time power grid simulation ...
Dennis Rösch, Kevin Schäfer, Steffen Nicolai   +2 more
doaj   +1 more source

Identification and characterization of a gene conferring stress resistance in Escherichia coli and cyanobacteria

FEBS Open Bio, EarlyView.
Functional screening identified PcSyn14890, a cyanobacteria‐specific protein that enhances growth and stress resistance in E. coli and Synechocystis PCC6803. Although we expected it to function as a molecular chaperone, it was unable to protect against thermal aggregation of GAPDH.
Akiyo Yamada, Takaaki Tanaka, Manaka Uehara, Keigo Nakano, Ken Morishima, Rintaro Inoue, Masaaki Sugiyama, Masafumi Yohda   +7 more
wiley   +1 more source

Electric Vehicle Communities for Electric Market [PDF]

World Electric Vehicle Journal, 2010
In this work is proposed the design of a system to create and handle an Electric Vehicle (EV) community, based on social networks collaborative approach and a credit mechanism to incentive participation and divide profits. This system is part of a V2G (Vehicle-to-Grid) module that allows EV owners to be aggregated in communities and participate in the ...
João C. Ferreira, João L. Afonso
openaire   +2 more sources

Self‐Regulated Learning Meets AI: Reinterpreting Self‐Regulation, Co‐Regulation, and Socially Shared Regulation in Human–AI Interaction

New Directions for Adult and Continuing Education, EarlyView.
ABSTRACT Advancing artificial intelligence (AI) has transformed learning and work, yet higher education and professional development programs have not systematically equipped learners for AI‐prevalent environments. This lack of preparation creates uncertainty regarding control, responsibility, trust, and accountability.
Moon‐Heum Cho, Jerusalem Merkebu
wiley   +1 more source

Ultimate bounded observer-based control of electrical vehicle driven by DC motor system with unmatched load torque

Advances in Mechanical Engineering
The unmatched perturbation of load torque is the essential problem in the Electrical Vehicle (EV) based DC motor which requires deep control concern for solution.
Akram H Hameed, Shibly A Al-Samarraie, Amjad J Humaidi   +2 more
doaj   +1 more source

Thalamo‐Lesional Connectivity Signatures of Bilateral Tonic–Clonic Seizures in Focal Cortical Dysplasia‐Related Epilepsy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objectives Focal cortical dysplasia (FCD) is the most common etiology of drug‐resistant epilepsy in children. Focal to bilateral tonic–clonic seizures (FBTCS) mark a high risk of drug‐resistant epilepsy and involve thalamocortical circuitry in their generation and propagation.
Hua Xie, Xiaotong Li, Venkata Sita Priyanka Illapani, Leigh N. Sepeta, Jason Schroeder, Chima O. Oluigbo, Madison M. Berl, William D. Gaillard, Nathan T. Cohen   +8 more
wiley   +1 more source