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Electron Microscopy Techniques
2014This chapter introduces the basic concepts of electron microscopy (EM), which comprises an extensive toolbox for characterizing the size, three-dimensional shape, composition, or crystal structure of nanoparticles.
van Huis, M.A., Friedrich, H.
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Transmission Electron Microscopy
Current Protocols in Microbiology, 2006AbstractTransmission electron microscopy has long been an important analytical tool in the field of microbiology. This unit describes preparation techniques for examining particulate samples as well as samples presenting more complex ultrastructural considerations that require analysis in thin sections.
Robert C, Burghardt, Robert, Droleskey
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Electron Microscopy of Archaea
2007Publisher Summary The information-processing systems of Archaea are surprisingly similar to, but much simpler than, the analogous systems in Eucarya. These similarities, discovered via genomics, indicate that the structural studies of Archaea may yield insights into the fundamental structures and mechanisms of the human branch in the tree of life ...
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Correlated Light Microscopy and Electron Microscopy
2012Understanding where, when, and how biomolecules (inter)act is crucial to uncover fundamental mechanisms in cell biology. Recent developments in fluorescence light microscopy (FLM) allow protein imaging in living cells and at the near molecular level. However, fluorescence microscopy only reveals selected biomolecules or organelles but not the (ultra ...
Sjollema, Klaas A. +4 more
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Ultramicroscopy, 1982
Abstract A brief review of significant attempts to overcome the problem of radiation damage is given, which indicates cryo electron microscopy as a likely solution. The need for clear understanding of the physical mechanism of cryo electron microscopy is stressed, and possible means to achieve such an understanding is indicated.
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Abstract A brief review of significant attempts to overcome the problem of radiation damage is given, which indicates cryo electron microscopy as a likely solution. The need for clear understanding of the physical mechanism of cryo electron microscopy is stressed, and possible means to achieve such an understanding is indicated.
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Electron Microscopy of Glomerulonephritis
1976The events occurring in glomerulonephritis produce a complex and complicated pattern of glomerular changes. Though these changes can be readily appreciated by light microscopy, many are too subtle to be clearly discerned. Only with the advent of electron microscopy did it become possible to describe the glomerular events with considerable accuracy and ...
J, Churg, E, Grishman
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Electron Microscopy of Integrins
2007Integrins are a family of heterodimeric, cell-surface receptors that mediate interactions between the cytoskeleton and the extracellular matrix. We have used electron microscopy and single-particle image analysis combined with molecular modeling to investigate the structures of the full-length integrin alpha(IIb)beta(3) and the ectodomain of alpha(V ...
Brian D, Adair, Mark, Yeager
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Bacteriophage Electron Microscopy
2012Since the advent of the electron microscope approximately 70 years ago, bacterial viruses and electron microscopy are inextricably linked. Electron microscopy proved that bacteriophages are particulate and viral in nature, are complex in size and shape, and have intracellular development cycles and assembly pathways.
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Electron Microscopy of Viruses
2010Electron microscopy is widely used in virology because viruses are generally too small for a direct inspection by light microscopy. Analysis of virus morphology is necessary in many circumstances, e.g., for the diagnosis of a virus in particular clinical situations or the analysis of virus entry and assembly. Moreover, quality control of virus particle
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Fixation in Electron Microscopy
Nature, 1955MANY specimens for electron microscopical examination are in the form of suspensions in salt solution. These are commonly dried on the specimen support film, and the unwanted salt crystals are removed by washing, with resultant uncertainties due to loss of material during the process, which losses may well be selective.
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