Results 31 to 40 of about 873,322 (297)

Assembly mechanism of the pleomorphic immature poxvirus scaffold

Nature Communications, 2022
Immature poxviruses are characterized by nonicosahedral semiordered protein scaffolds critical for morphogenesis. Here, the authors use cryo-EM structures of Vaccinia virus D13 scaffold intermediates to explain their assembly mechanism.
Jaekyung Hyun, Hideyuki Matsunami, Tae Gyun Kim, Matthias Wolf   +3 more
doaj   +1 more source

Reciprocal control of viral infection and phosphoinositide dynamics

FEBS Letters, EarlyView.
Phosphoinositides, although scarce, regulate key cellular processes, including membrane dynamics and signaling. Viruses exploit these lipids to support their entry, replication, assembly, and egress. The central role of phosphoinositides in infection highlights phosphoinositide metabolism as a promising antiviral target.
Marie Déborah Bancilhon, Bruno Mesmin
wiley   +1 more source

Interplay between circadian and other transcription factors—Implications for cycling transcriptome reprogramming

FEBS Letters, EarlyView.
This perspective highlights emerging insights into how the circadian transcription factor CLOCK:BMAL1 regulates chromatin architecture, cooperates with other transcription factors, and coordinates enhancer dynamics. We propose an updated framework for how circadian transcription factors operate within dynamic and multifactorial chromatin landscapes ...
Xinyu Y. Nie, Jerome S. Menet
wiley   +1 more source

Noise2Atom: unsupervised denoising for scanning transmission electron microscopy images

Applied Microscopy, 2020
We propose an effective deep learning model to denoise scanning transmission electron microscopy (STEM) image series, named Noise2Atom, to map images from a source domain S $\mathcal {S}$ to a target domain C $\mathcal {C}$ , where S $\mathcal {S}$ is ...
Feng Wang, Trond R. Henninen, Debora Keller, Rolf Erni   +3 more
doaj   +1 more source

Performance evaluation of pipelines for mapping, variant calling and interval padding, for the analysis of NGS germline panels

BMC Bioinformatics, 2021
Background Next-generation sequencing (NGS) represents a significant advancement in clinical genetics. However, its use creates several technical, data interpretation and management challenges.
Maria Zanti, Kyriaki Michailidou, Maria A. Loizidou, Christina Machattou, Panagiota Pirpa, Kyproula Christodoulou, George M. Spyrou, Kyriacos Kyriacou, Andreas Hadjisavvas   +8 more
doaj   +1 more source

Immune Electron Microscopy

Experimental Biology and Medicine, 1960
SummaryA method is described by which immune systems can be studied at subcellular level with the electron microscope, using a ferritin-antibody conjugate. A conjugated immune serum to Staphylococcus aureus was applied to homologous organisms, embedded in methacrylate with uranium and thin sections cut.
C W, SMITH, J F, METZGER, S I, ZACKS, A, KASE   +3 more
openaire   +2 more sources

Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing

FEBS Letters, EarlyView.
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley   +1 more source

Electrostatic Electron Microscopy. III [PDF]

Journal of Applied Physics, 1943
This article, the final one of a series on the design of electrostatic electron microscopes, contains a description of an instrument which illustrates the principles previously discussed. The microscope described is believed to be the first constructed with the object of providing the greatest of simplicity in construction, operation, and maintenance ...
Bachman, C. H., Ramo, Simon
openaire   +6 more sources

Substrate specificity of Burkholderia pseudomallei multidrug transporters is influenced by the hydrophilic patch in the substrate‐binding pocket

FEBS Letters, EarlyView.
Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley   +1 more source

Three-dimensional distribution of individual atoms in the channels of beryl

Communications Materials
Single atom detection in nanoporous materials is a significant challenge, particularly due to their sensitivity to electron irradiation. Here, natural beryl (Be3Al2Si6O18) is used as a model system to quantitatively analyse the occupancy of its atomic ...
Daniel Knez, Christian Gspan, Nikola Šimić, Stefan Mitsche, Harald Fitzek, Karl Gatterer, Helmar Wiltsche, Gerald Kothleitner, Werner Grogger, Ferdinand Hofer   +9 more
doaj   +1 more source