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Improved Isolation of Ultra-High-Molecular-Weight Genomic DNA Suitable for Third-Generation Sequencing. [PDF]

open access: yesMicroorganisms
Ova AÖ   +7 more
europepmc   +1 more source

Pulsed Field Gel Electrophoresis

2003
Pulsed- field gel electrophoresis (PFGE) was first described by Schwartz and Cantor (1) It is now an umbrella term for the alternating of an electric field in more than one direction through a solid matrix to achieve the separation of DNA fragments.
A J, Hillier, B E, Davidson
  +8 more sources

Pulsed-field gel electrophoresis

Nature Protocols, 2007
This protocol describes pulsed-field gel electrophoresis (PFGE), a method developed for separation of large DNA molecules. Whereas standard DNA gel electrophoresis commonly resolves fragments up to approximately 50 kb in size, PFGE fractionates DNA molecules up to 10 Mb.
Jill, Herschleb   +2 more
openaire   +3 more sources

Pulsed field sequencing gel electrophoresis

ELECTROPHORESIS, 1992
AbstractThe effect of pulsed fields on sequencing gel electrophoresis is investigated, using DNA fragment markers ranging in size from 20 to 6557 bases. For high continuous electric fields (5000 V/55 cm) band inversion is observed in which fragments larger than 4000 bases migrate faster than those of 800–1000 bases.
E, Brassard, C, Turmel, J, Noolandi
openaire   +2 more sources

Pulsed field gel electrophoresis

Analytical Chemistry, 1991
The development of pulsed field gel electrophoresis has increased by 2 orders of magnitude the size of DNA molecules that can be routinely fractionated and analyzed. This increase is of major importance to molecular biology because it simplifies many previously laborious investigations and makes possible many new ones.
openaire   +4 more sources

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