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Impact of pulsed electric field assisted vacuum impregnation of chitooligosaccharide-epigallocatechin gallate conjugate and hydrocolloids on quality and shelf-life of Asian hard clam. [PDF]
Singh A +5 more
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Low occurrence and clonal relatedness of multi-drug resistant <i>Escherichia coli</i> carrying transmissible colistin resistance <i>mcr-1</i> genes in Ugandan poultry. [PDF]
Wainaina M +8 more
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Improved Isolation of Ultra-High-Molecular-Weight Genomic DNA Suitable for Third-Generation Sequencing. [PDF]
Ova AÖ +7 more
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Draft genome sequences of five <i>Bartonella henselae</i> strains from shelter cats in Osaka, Japan. [PDF]
Umeda K, Hirai Y.
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Pulsed Field Gel Electrophoresis
2003Pulsed- field gel electrophoresis (PFGE) was first described by Schwartz and Cantor (1) It is now an umbrella term for the alternating of an electric field in more than one direction through a solid matrix to achieve the separation of DNA fragments.
A J, Hillier, B E, Davidson
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Pulsed-field gel electrophoresis
Nature Protocols, 2007This protocol describes pulsed-field gel electrophoresis (PFGE), a method developed for separation of large DNA molecules. Whereas standard DNA gel electrophoresis commonly resolves fragments up to approximately 50 kb in size, PFGE fractionates DNA molecules up to 10 Mb.
Jill, Herschleb +2 more
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Pulsed field sequencing gel electrophoresis
ELECTROPHORESIS, 1992AbstractThe effect of pulsed fields on sequencing gel electrophoresis is investigated, using DNA fragment markers ranging in size from 20 to 6557 bases. For high continuous electric fields (5000 V/55 cm) band inversion is observed in which fragments larger than 4000 bases migrate faster than those of 800–1000 bases.
E, Brassard, C, Turmel, J, Noolandi
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Pulsed field gel electrophoresis
Analytical Chemistry, 1991The development of pulsed field gel electrophoresis has increased by 2 orders of magnitude the size of DNA molecules that can be routinely fractionated and analyzed. This increase is of major importance to molecular biology because it simplifies many previously laborious investigations and makes possible many new ones.
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