Results 1 to 10 of about 30,790 (245)

The Application of Pulsed Field Gel Electrophoresis in Clinical Studies [PDF]

Journal of Clinical and Diagnostic Research, 2016
Pulsed-field gel electrophoresis is a method applied in separating large segments of deoxyribonucleotide using an alternating and cross field. In a uniform magnetic field, components larger than 50kb pass a route through the gel and since the movement ...
Elaheh Gholami Parizad, Eskandar Gholami Parizad, Azar Valizadeh   +2 more
doaj   +2 more sources

Data on antibiogram and resistance genes harboured by Salmonella strains and their Pulsed-field gel electrophoresis clusters [PDF]

Data in Brief, 2018
This article describes the Pulsed-field gel electrophoresis clustering of the predominant Salmonella strains (Salmonella ser. Albany, Salmonella ser. Brancaster, and Salmonella ser.
Li-Oon Chuah, Ahamed-Kamal Shamila Syuhada, Ismail Mohamad Suhaimi, Tajudin Farah Hanim, Gulam Rusul   +4 more
doaj   +2 more sources

Methicillin-resistant Staphylococcus aureus in a Turkish hospital: characterization of clonal types and antibiotic susceptibility

Journal of Infection in Developing Countries, 2021
Introduction: The assessment of the clonal spread of methicillin resistant Staphylococcus aureus (MRSA) in nosocomial and community-acquired infections through characterization of the isolates is critical for tracking the evolution of the epidemics ...
Serdar Güngör, Alper Karagöz, Nadir Koçak, Tutku Arslantaş   +3 more
doaj   +1 more source

Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients

Brazilian Journal of Infectious Diseases, 2013
Burkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients.
Luana Pretto, Fernanda de-Paris, Alice Beatriz Mombach Pinheiro Machado, Andreza Francisco Martins, Afonso Luís Barth   +4 more
doaj   +3 more sources

Agarose Gel Electrophoresis-Based RAPD-PCR—An Optimization of the Conditions to Rapidly Detect Similarity of the Alert Pathogens for the Purpose of Epidemiological Studies

Gels, 2022
Agarose gel electrophoresis is a well-known tool to detect DNA fragments amplified in polymerase chain reaction (PCR). Its usefulness has also been confirmed for epidemiological studies based on restriction fragments length polymorphism (RFLP), usually ...
Tomasz Bogiel, Agnieszka Mikucka, Piotr Kanarek   +2 more
doaj   +1 more source

Pulse Field Gel Electrophoresis [PDF]

, 2014
Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction.
Batu K, Sharma-Kuinkel, Thomas H, Rude, Vance G, Fowler   +2 more
openaire   +2 more sources

Agarose Gel Analysis of 15–40-kb PCR Amplimers

BioTechniques, 1996
Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to ...
Valerie Burland, Foner P. Curtis, Noriko Kusukawa   +2 more
doaj   +1 more source

Nosocomial Transmission of Group B Streptococci Proven by Positive Environmental Culture

Oman Medical Journal, 2014
Objective: Neonates usually acquire Group B streptococcal infection vertically from the maternal birth canal during delivery. In January 2010, a Group B streptococcal outbreak investigation was conducted in response to an increased number of clinical ...
Amal Al-Maani, Laurie Streitenberger, Megan Clarke, Yvonne C. W. Yau, Danuta Kovach, Rick Wray, Anne Matlow   +6 more
doaj   +1 more source

Streptococcus pneumoniae serotype 3 genotypes in invasive isolates from Colombia

Biomédica: revista del Instituto Nacional de Salud, 2021
Introduction: Streptococcus pneumoniae serotype 3 is an important cause of pneumonia, bacteremia, and meningitis. Objective: To establish the circulating genotypes of S.
Zonia Katerin Alarcón, Carolina Duarte, Olga Sanabria, Jaime Moreno   +3 more
doaj   +1 more source

Pulsed field gel electrophoresis protocol for typing of uropathogenic Escherichia coli [PDF]

فصلنامه دانشگاه علوم پزشکی جهرم, 2011
Introduction: Pulsed Field Gel Electrophoresis (PFGE) is one of the best typing methods which is highly effective in molecular epidemiological studies in different areas. All bacterial isolates are typeable by PFGE and the results are highly reproducible.
Mojtaba Anvarinejad, Shohreh Farshad, vahhab Alborzi, Reza Ranjbar, Marzeyeh Hoseini   +4 more
doaj   +1 more source