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Pulsed-Field Gel Electrophoresis of Salmonella enterica
, 2015 Pulsed-field gel electrophoresis (PFGE) is considered a "gold standard" for the molecular characterization of a number of bacterial strains. Its strength relies on its high discriminatory power, together with its high reproducibility. For many years, an international network, PulseNet International, allows the rapid comparison of PFGE data obtained all
CAMARDA, Antonio +5 more
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Pulsed-Field Gel Electrophoresis for Listeria monocytogenes
, 2015 Pulsed-Field Gel Electrophoresis (PFGE) is a molecular subtyping method with high discriminatory power, reproducibility, and epidemiological concordance for the subtyping of Listeria monocytogenes and other bacteria. PFGE uses rare-cutting restriction enzymes (macrorestriction) that cut the genomic DNA, usually resulting in 6-25 DNA fragments ranging ...
Laura, Luque-Sastre +2 more
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Pulsed-Field Gel Electrophoresis of Staphylococcus aureus
2015For many bacterial pathogens, including Staphylococcus aureus, pulsed-field gel electrophoresis (PFGE) is a molecular typing method widely used in surveillance and epidemiological investigations. The general principle of PFGE involves creating large DNA fragments from intact bacterial chromosomes using rare cutting restriction endonucleases.
George R, Golding +3 more
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Pulsed-Field Gel Electrophoresis of Yersinia pestis
2015Yersinia pestis is a human pathogen and can cause serious disease. Biosafety level 3 (BSL3) is required when handling this microorganism and all work requires a biological safety cabinet. For pulsed-field gel electrophoresis (PFGE), dedicated BSL3 PFGE equipment or a documented procedure that ensures that all viable bacteria are inactivated is required.
Tamara, Revazishvili, Judith A, Johnson
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Pulsed-Field Gel Electrophoresis of Pseudomonas aeruginosa
2015Pulsed-field gel electrophoresis (PFGE) is a technique used for the separation of high molecular weight restriction fragments from digested bacterial genomic DNA on a gel matrix by applying an electric field that periodically changes direction. PFGE has been shown to be the most discriminatory and reproducible bacterial strain typing technique ...
Honghua, Hu, Jim, Manos
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Pulsed Field Gel Electrophoresis of Staphylococcus epidermidis
2013Pulsed field gel electrophoresis (PFGE) is one of the older methods for the molecular characterization and comparison of microorganisms including bacteria. Nevertheless, PFGE continues to be recognized as the gold standard for molecular typing due to output spanning >90 % of the bacterial genome and standardized protocols and reagents applicable to a ...
Richard V, Goering, Paul D, Fey
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Pulsed-Field Gel Electrophoresis of Bacterial Chromosomes
2013The separation of fragments of DNA by agarose gel electrophoresis is integral to laboratory life. Nevertheless, standard agarose gel electrophoresis cannot resolve fragments bigger than 50 kb. Pulsed-field gel electrophoresis is a technique that has been developed to overcome the limitations of standard agarose gel electrophoresis.
Julia S P, Mawer, David R F, Leach
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Pulsed Field Ablation for the Treatment of Atrial Fibrillation: PULSED AF Pivotal Trial
Circulation, 2023Prashanthan Sanders +2 more
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Markers for Pulsed-field Gel Electrophoresis
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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