Results 11 to 20 of about 43,524 (303)

Methicillin-resistant Staphylococcus aureus in a Turkish hospital: characterization of clonal types and antibiotic susceptibility

Journal of Infection in Developing Countries, 2021
Introduction: The assessment of the clonal spread of methicillin resistant Staphylococcus aureus (MRSA) in nosocomial and community-acquired infections through characterization of the isolates is critical for tracking the evolution of the epidemics ...
Serdar Güngör, Alper Karagöz, Nadir Koçak, Tutku Arslantaş   +3 more
doaj   +1 more source

Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients

Brazilian Journal of Infectious Diseases, 2013
Burkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients.
Luana Pretto, Fernanda de-Paris, Alice Beatriz Mombach Pinheiro Machado, Andreza Francisco Martins, Afonso Luís Barth   +4 more
doaj   +3 more sources

The Application of Pulsed Field Gel Electrophoresis in Clinical Studies [PDF]

Journal of Clinical and Diagnostic Research, 2016
Pulsed-field gel electrophoresis is a method applied in separating large segments of deoxyribonucleotide using an alternating and cross field. In a uniform magnetic field, components larger than 50kb pass a route through the gel and since the movement ...
Elaheh Gholami Parizad, Eskandar Gholami Parizad, Azar Valizadeh   +2 more
doaj   +1 more source

Agarose Gel Electrophoresis-Based RAPD-PCR—An Optimization of the Conditions to Rapidly Detect Similarity of the Alert Pathogens for the Purpose of Epidemiological Studies

Gels, 2022
Agarose gel electrophoresis is a well-known tool to detect DNA fragments amplified in polymerase chain reaction (PCR). Its usefulness has also been confirmed for epidemiological studies based on restriction fragments length polymorphism (RFLP), usually ...
Tomasz Bogiel, Agnieszka Mikucka, Piotr Kanarek   +2 more
doaj   +1 more source

Application of Pulsed Field Gel Electrophoresis to Determine γ-ray-induced Double-strand Breaks in Yeast Chromosomal Molecules [PDF]

, 1993
The frequency of DNA double-strand breaks (dsb) was determined in yeast cells exposed to γ-rays under anoxic conditions. Genomic DNA of treated cells was separated by pulsed field gel electrophoresis, and two different approaches for the evaluation of ...
Beisker, W., Eckardt-Schupp, F., Kellerer, Albrecht M., Eckard-Schupp, F., Friedl, A.A., Kellerer, A.M., Hahn, K.R., Friedl, Anna A., Hahn, K.   +8 more
core   +1 more source

Pulse Field Gel Electrophoresis [PDF]

, 2014
Pulse Field Gel Electrophoresis (PFGE) is a powerful genotyping technique used for the separation of large DNA molecules (entire genomic DNA) after digesting it with unique restriction enzymes and applying to a gel matrix under the electric field that periodically changes direction.
Batu K, Sharma-Kuinkel, Thomas H, Rude, Vance G, Fowler   +2 more
openaire   +2 more sources

Data on antibiogram and resistance genes harboured by Salmonella strains and their Pulsed-field gel electrophoresis clusters

Data in Brief, 2018
This article describes the Pulsed-field gel electrophoresis clustering of the predominant Salmonella strains (Salmonella ser. Albany, Salmonella ser. Brancaster, and Salmonella ser.
Li-Oon Chuah, Ahamed-Kamal Shamila Syuhada, Ismail Mohamad Suhaimi, Tajudin Farah Hanim, Gulam Rusul   +4 more
doaj   +1 more source

Localization of the genes for tumor necrosis factor and lymphotoxin between the HLA classI and III regions by field inversion gel electrophoresis [PDF]

, 1988
To clarify the position of the TNFA and TNFB genes on the HLA map, we have assigned TNFA to large DNA restriction fragments separated by field inversion gel electrophoresis, which hybridize with either class III- or class I-specific probes as well. These
Bloemer, K, Bloemer, Katharina, Ziegler, Andreas, Ragoussis, Jiannis, Ragoussis, J, Weiss, Elisabeth H., Weiss, EH, Ziegler, A   +7 more
core   +1 more source

Agarose Gel Analysis of 15–40-kb PCR Amplimers

BioTechniques, 1996
Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to ...
Valerie Burland, Foner P. Curtis, Noriko Kusukawa   +2 more
doaj   +1 more source

A Physical Map Including a New Class I Gene (cda12) of the Human Major Histocompatibility Complex (A2/313 Haplotype) Derived from a Monosomy 6 Mutant Cell Line [PDF]

, 1989
To avoid interpretative problems due to restriction fragment length polymorphisms, the monosomy 6 mutant cell line BM19.7 was employed to establish a molecular map of the human major histocompatibility (HLA) complex in the A2,B13,Bw4,DRw6,DRw52,DQw1,DPw2
Pohla, Heike, Bloemer, K, Messer, Gerald, Bloemer, Katharina, Messer, G, Ziegler, Andreas, Ragoussis, Jiannis, Ragoussis, J, Weiss, Elisabeth H., Pohla, H, Weiss, EH, Ziegler, A   +11 more
core   +1 more source