Results 41 to 50 of about 43,524 (303)

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Enterococcus faecalis Clones in Poultry and in Humans with Urinary Tract Infections, Vietnam

Emerging Infectious Diseases, 2012
Enterococcus spp. as pathogens have increased, but the sources of infection often remain unclear. To investigate whether poultry might be a reservoir for E. faecalis–associated urinary tract infections (UTIs) in humans, we characterized E.
Louise Ladefoged Poulsen, Magne Bisgaard, Nguyen Thai Son, Nguyen Vu Trung, Hoang Manh An, Anders Dalsgaard   +5 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Characterization of Toxigenic Vibrio cholerae from Haiti, 2010–2011

Emerging Infectious Diseases, 2011
In October 2010, the US Centers for Disease Control and Prevention received reports of cases of severe watery diarrhea in Haiti. The cause was confirmed to be toxigenic Vibrio cholerae, serogroup O1, serotype Ogawa, biotype El Tor.
Deborah Talkington, Cheryl Bopp, Cheryl Tarr, Michele B. Parsons, Georges Dahourou, Molly Freeman, Kevin Joyce, Maryann Turnsek, Nancy Garrett, Michael Humphrys, Gerardo Gomez, Steven Stroika, Jacques Boncy, Benjamin Ochieng, Joseph Oundo, John D. Klena, Anthony Smith, Karen H. Keddy, Peter Gerner-Smidt   +18 more
doaj   +1 more source

Higher isolation of NDM-1 producing Acinetobacter baumannii from the sewage of the hospitals in Beijing. [PDF]

PLoS ONE, 2014
Multidrug resistant microbes present in the environment are a potential public health risk. In this study, we investigate the presence of New Delhi metallo-β-lactamase 1 (NDM-1) producing bacteria in the 99 water samples in Beijing City, including river ...
Chuanfu Zhang, Shaofu Qiu, Yong Wang, Lihua Qi, Rongzhang Hao, Xuelin Liu, Yun Shi, Xiaofeng Hu, Daizhi An, Zhenjun Li, Peng Li, Ligui Wang, Jiajun Cui, Pan Wang, Liuyu Huang, John D Klena, Hongbin Song   +16 more
doaj   +1 more source

Use of AFLP and PFGE to discriminate between Salmonella enterica serovar Typhimurium DT126 isolates from separate food-related outbreaks in Australia

, 2005
In 2001 Salmonella enterica serovar Typhimurium definitive phage-type (DT) 126 was isolated at higher frequency in Australia compared to other S. Typhimurium phage types and in comparison to previous years.
Heuzenroeder, M., Ross, I.
core   +1 more source

Exploring an Alternative to mRNA Vaccine Cold Chain Storage: MRNA‐Lipid Nanoparticle Stability When Dried in a Polymer Matrix

Advanced Functional Materials, EarlyView.
The nanostructure, size, and function of mRNA‐loaded lipid nanoparticles are evaluated before drying, within polymer microneedles, and after rehydration. The results reveal the polymer and LNP loadings required to recover nanostructure and preserve the delivery performance in dry‐state formulations.
Brendan P. Dyett, Shahad K. Alsaiari, John L. Daristotle, Timothy A. Forster, Alicia Lau, Dhruv Varshney, Rania Afaneh, Andrew R. Hanna, Charles Sloane, David Mankus, Haitao Yu, Jiali Zhai, DongSoo Yun, Lisa H. Tostanoski, Abigail Lytton‐Jean, Dan H. Barouch, Charlotte E. Conn, Robert Langer, Ana Jaklenec, Calum J. Drummond   +19 more
wiley   +1 more source

Genetic similarity of Burkholderia cenocepacia from cystic fibrosis patients

Brazilian Journal of Infectious Diseases, 2013
Burkholderia cenocepacia may cause serious infections in patients with cystic fibrosis, and this microorganism can be highly transmissible. Pulsed-field gel electrophoresis is widely used to study the dynamics of strain spread in cystic fibrosis patients.
Luana Pretto, Fernanda de-Paris, Alice Beatriz Mombach Pinheiro Machado, Andreza Francisco Martins, Afonso Luís Barth   +4 more
doaj  

Pulsed-field gel electrophoresis of circular DNA [PDF]

Nucleic Acids Research, 1989
Mobility of supercoiled (form I) and nicked circular (form II) plasmid DNAs was determined on two major forms of pulsed-field electrophoresis, CHEF and OFAGE. Plasmids with molecular lengths ranging from 2.30 to 17.8 kilobase pairs (kb) were used with Saccharomyces cerevisiae chromosomes as standards.
J S, Simske, S, Scherer
openaire   +2 more sources

A Long‐Lived Human Neurovascular PENTA Culture Model Captures Incomplete Vascular Repair and Glia‐Associated Signaling After Traumatic Brain Injury

Advanced Healthcare Materials, EarlyView.
A long‐lived, five‐cell‐type human neurovascular (PENTA) model recreates vascular disorganization and incomplete repair after traumatic brain injury (TBI). By integrating endothelial, glial, neuronal, and immune components within a 3D scaffold, the platform enables time‐resolved analysis of neurovascular remodeling and provides a human‐relevant system ...
Daniel S. Hinrichsen, Sunghyun Jun, Colleen M. Arrasmith, Charitha C. Anamala, Mitchell D. Thielen, Anapaula Garcia, Volha Liaudanskaya   +6 more
wiley   +1 more source