Results 71 to 80 of about 43,524 (303)

Pulsed-field gel electrophoresis studies of Rhizobiaceae

, 1989
The migration properties of circular, covalently closed (ccc) and linear DNAs were studied in pulsed-field gel electrophoresis as a model system for resolving large plasmids in the Rhizobiaceae. The effects of varying pulse time and agarose concentration
Sobral, Bruno
core   +1 more source

Neutral Genomic Microevolution of a Recently Emerged Pathogen, Salmonella enterica Serovar Agona [PDF]

, 2013
Salmonella enterica serovar Agona has caused multiple food-borne outbreaks of gastroenteritis since it was first isolated in 1952. We analyzed the genomes of 73 isolates from global sources, comparing five distinct outbreaks with sporadic infections as ...
Eva Litrup (404346), Murphy, Ronan, Fanning, S, Mark Achtman, Angela McCann (404345), Sylvain Brisse (87594), Brown, D, Zhou, Z, Cormican, Martin, Brisse, Sylvain, Mark Achtman (126786), Guttman, David S., Zhemin Zhou (157214), Ronan Murphy (404347), Brown, Derek, Litrup, Eva, Seamus Fanning (5651275), McCann, Angela, Brisse, S, David S. Guttman (131322), Achtman, Mark, McCann, A, Derek Brown (404349), Sylvain Brisse, Zhou, Zhemin, Litrup, E, Murphy, R, Angela McCann, Zhemin Zhou, David S Guttman, Martin Cormican, Guttman, DS, Eva Litrup, Ronan Murphy, Seamus Fanning, Derek Brown, Fanning, Seamus, Achtman, M, Cormican, M, Martin Cormican (404348)   +39 more
core   +1 more source

Enhanced Glycolysis‐Driven Histone H3K18 Lactylation Regulates Epileptogenesis by Modulating the E3 Ubiquitin Ligase COP1

Advanced Science, EarlyView.
Neuronal PKM2‐driven glycolysis generates excess lactate that triggers histone H3K18 lactylation (H3K18la), establishing a pathogenic metabolic‐epigenetic axis in epilepsy. Elevated H3K18la enriches the Cop1 promoter, transcriptionally upregulating the E3 ubiquitin ligase COP1, which subsequently drives proteasomal degradation of GABAARβ2 and impairs ...
Yuan Meng, Yanlin Guo, Liumi Jiang, Jing Luo, Yan Liu, Ziwei Yuan, Pingyang Ke, Ran Duan, Fei Xiao   +8 more
wiley   +1 more source

Clonal Multidrug-Resistant Corynebacterium striatum Strains, Italy

Emerging Infectious Diseases, 2009
We assessed the clinical relevance and performed molecular characterization of 36 multidrug-resistant strains of Corynebacterium striatum. Pulsed-field gel electrophoresis confirmed a single clone, possessing erm(X), tetA/B, cmxA/B, and aphA1 genes, but ...
Floriana Campanile, Edoardo Carretto, Daniela Barbarini, Annalisa Grigis, Marco Falcone, Antonio Goglio, Mario Venditti, Stefania Stefani   +7 more
doaj   +1 more source

Pulsed-field gel electrophoresis profiles and dendrogram.

, 2013
Pulsed-field gel electrophoresis profiles and dendrogram of the 41 KPC-2-producing K. pneumoniae isolates. One major pulsotype is shown using the 80% similarity as the cut-off. All these KPC-2-producing K. pneumoniae isolates are ST11.
Chang-Phone Fung (328475), Po-Liang Lu (5309), Sheng-Kang Chiu (436980), L. Kristopher Siu (300243), Tsu-Lan Wu (315191), Jung-Chung Lin (298849), Yin-Ching Chuang (95603), Lih-Shinn Wang (436981), Jann-Tay Wang (195783), Kuo-Ming Yeh (359326)   +9 more
core   +1 more source

Cigarette Smoke‐Induced Alveolar Macrophage Senescence via GEM/SIRT3‐Mediated Mitochondrial Dysfunction

Advanced Science, EarlyView.
Cigarette smoke accelerates the aging of immune cells in the lung. By combining human single‐cell sequencing, cell culture, and mouse models, the authors show that the protein GEM drives mitochondrial damage and senescence in alveolar macrophages by suppressing SIRT3.
Jin Wang, Meidan Wei, Xiangrong Song, Jiaxin Zhang, Lisha Zhang, Sixian Chen, Yaoyu Hu, Hui Gao, Jianxiang Li   +8 more
wiley   +1 more source

Human-to-Dog Transmission of Methicillin-Resistant Staphylococcus aureus

Emerging Infectious Diseases, 2004
Methicillin-resistant Staphylococcus aureus (MRSA) was cultured from the nose of a healthy dog whose owner was colonized with MRSA while she worked in a Dutch nursing home.
Engeline van Duijkeren, Maurice J.H.M. Wolfhagen, Adrienne T.A. Box, Max E.O.C. Heck, Wim J.B. Wannet, Ad C. Fluit   +5 more
doaj   +1 more source

Pulsed field gel electrophoresis on frozen tumour tissue sections.

, 1992
The application of pulsed field gel electrophoresis (PFGE) to the molecular genetic analysis of solid tumours has been restricted by the requirement for whole single cells as a DNA source.
Wainscoat, JS, Boultwood, Jacqueline, Boultwood, J, Kaklamanis, L, Gatter, KC   +4 more
core   +1 more source

S100A14 in Tumor‐Derived EVs Targets PIAS3 to Reprogram Astrocytes and Induce Immunosuppressive Microenvironment Promoting Brain Metastasis and Germacrone Reversal Effect

Advanced Science, EarlyView.
This study identifies S100A14 in tumor‐derived exosomes as a key driver of brain metastasis. S100A14 targets PIAS3 in astrocytes, activating STAT3 signaling and promoting immunosuppressive MDSCs recruitment via chemokine secretion. Germacrone, a natural compound, binds S100A14 to disrupt this axis, effectively inhibiting brain metastasis with low ...
Qian Feng, Xia Yang, Lin An, Xue‐Yu Wang, Ming‐Rui Wang, Zhuo‐Hong Cai, Cai‐Jun Xie, Li‐Jun Qiao, You‐Bi Shen, Xi‐Min Liang, Rong Zhang, Cai‐Yan Wang, Zhong‐Qiu Liu, Rong‐Rong Zhang   +13 more
wiley   +1 more source

Fecal Colonization with Vancomycin-Resistant Enterococci in Australia

Emerging Infectious Diseases, 2000
To assess the rate of fecal vancomycin-resistant enterococci (VRE) colonization in Australia, we examined specimens from 1,085 healthy volunteers. VRE was cultured from 2 (0.2%) of 1,085 specimens; both were vanB Enterococcus faecium, identical by pulsed-
Alexander A. Padiglione, Elizabeth A. Grabsch, Dianne Olden, Margaret Hellard, Martha I. Sinclair, Christopher K Fairley, M. Lindsay Grayson   +6 more
doaj   +1 more source