Results 181 to 190 of about 327,725 (237)

DepStep: An Efficient One-Step rRNA Depletion Workflow for RNA Sequencing in Non-model Organisms. [PDF]

Bio Protoc
Qasim SM, Sarin PL.
europepmc   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

A Selective History of Protein Sequencing and the Impact of Donald F. Hunt on Protein Sequencing by Tandem Mass Spectrometry. [PDF]

J Proteome Res
Yates JR.
europepmc   +1 more source

Increased Insulin Action, Glucose Metabolism and Muscle Function in Supervillin‐Knockout and Supervillin‐Mutant Mice

Cytoskeleton, EarlyView.
ABSTRACT We here describe mouse models with complementary homozygous Svil mutations. In skeletal muscle, Svil‐Mut mice express the Svil‐encoded N‐terminus fused to the βgal‐neo gene‐trap tag and lack the highly conserved archvillin C‐terminus; Svil‐KO mice lack expression of all known Svil‐encoded proteins; and Svil‐LoxP mice contain loxP sites for ...
Tara C. Smith, Lauren A. Tauer, Randall H. Friedline, Xiaodi Hu, Duy Tran, Yangyi E. Luo, Nacima Hadjout‐Rabi, Elisabeth R. Barton, Jason K. Kim, Elizabeth J. Luna   +9 more
wiley   +1 more source

Capturing the dynamics of STAT6 macrophage polarization using bioluminescence temporal signatures. [PDF]

Cell Syst
Zheng S, Chiang YH, Aldarondo D, Locke A, Gunaje J, Davis J, Fox ZR, Akpa BS, Wayne E.   +8 more
europepmc   +1 more source

Selective Modification of the Product Profile of Biocatalytic Hydrolyzed PET via Product‐Specific Medium Engineering

ChemSusChem, Volume 18, Issue 6, March 15, 2025.
The product profile of enzymatically hydrolyzed PET can be modified by medium engineering and thereby adapted to a desired product. TPA, MHET or BHET can be forced as the predominant product using a basic pH (blue), 25 % ethylene glycol (EG) and IsPETasewt (green) or ≥25 % EG and LCCICCG (pink), respectively.
Tobias Heinks, Katrin Hofmann, Simon Last, Igor Gamm, Luise Blach, Ren Wei, Uwe T. Bornscheuer, Christof Hamel, Jan von Langermann   +8 more
wiley   +1 more source

Molecular and serological evidence of systemic <i>Treponema pallidum</i> infection in wild western chimpanzees. [PDF]

iScience
Labarrere C, Hama HO, Orain N, Dotras L, Llana M, Hernandez-Aguilar RA, Fenollar F, Mediannikov O.   +7 more
europepmc   +1 more source

Comprehensive Evaluation of Commercial Antibodies for High Sensitivity Detection of EPO‐Doping on Western Blots

Drug Testing and Analysis, EarlyView.
A large‐scale evaluation of 164 commercially available anti‐human EPO antibodies was conducted to identify an alternative to clone AE7A5 anti‐EPO antibody used in doping control for the detection of erythropoiesis‐stimulating agents (ERAs) on Western blots.
Christian Reichel, Günter Gmeiner, Hans Geyer, Mario Thevis   +3 more
wiley   +1 more source