Results 11 to 20 of about 510,638 (275)

What Room for Two-Dimensional Gel-Based Proteomics in a Shotgun Proteomics World?

Proteomes, 2020
Two-dimensional gel electrophoresis was instrumental in the birth of proteomics in the late 1980s. However, it is now often considered as an outdated technique for proteomics—a thing of the past.
Katrin Marcus, Cécile Lelong, Thierry Rabilloud   +2 more
doaj   +1 more source

Separation of dog serum lipoproteins by ultracentrifugation, dextran sulfate precipitation, and paper electrophoresis*

Journal of Lipid Research, 1961
The separation of dog serum lipoproteins by ultracentrifugation at a density of 1.063 is hindered by the failure of the high density fraction to accumulate in the bottom portion of the centrifuge tube.
Toshio Sakagami, D.B. Zilversmit
doaj   +1 more source

Evaluation of gel chromatography for plasma lipoprotein fractionation

Journal of Lipid Research, 1970
The fractionation of lipoproteins of normal and hyperlipidemic subjects on a column of 2% agarose was compared with ultracentrifugation and paper electrophoresis procedures. The following results were obtained.
Teizo Sata, David L. Estrich, Peter D.S. Wood, Laurance W. Kinsell   +3 more
doaj   +1 more source

Flash-comet assay

MethodsX, 2020
In the present paper, we present a substantially revised protocol of the widely used SCGE assay performed under alkaline conditions. In our updated version of the comet assay, which we call the Flash-comet, LiOH is used instead of NaOH during the ...
Erik Bivehed, Björn Hellman
doaj   +1 more source

Results of conducting the primary seed production using the method of electrophoresis in the FSBSI “ARC “Donskoy

Зерновое хозяйство России, 2022
The current paper has presented the study and estimation results of winter bread wheat varieties in primary seed production (2019-2021) based on the method of electrophoresis.
T. I. Firsova, M. M. Kopus, Yu. G. Skvortsova, G. A. Filenko   +3 more
doaj   +1 more source

DNA electrophoresis studied with the cage model [PDF]

, 2001
The cage model for polymer reptation, proposed by Evans and Edwards, and its recent extension to model DNA electrophoresis, are studied by numerically exact computation of the drift velocities for polymers with a length L of up to 15 monomers.
A. van Heukelum, Barkema, Barkema, Barkema, Barkema, Bisseling, Carlon, de Gennes, Duke, Duke, Duke, Duke, Evans, G.T. Barkema, Hill, Kolomeisky, Kooiman, Kooiman, Newman, Prähofer, R.H. Bisseling, Rubinstein, Valiant, van Heukelum, van Leeuwen, Widom   +25 more
core   +5 more sources

Quantitative analysis of unconjugated and conjugated bile acids in duodenal fluid by densitometry after paper electrophoresis

Journal of Lipid Research, 1973
A new paper electrophoretic method for the separation of bile acids into five groups, (1) unconjugated, (2) glycine conjugates and (3) taurine conjugates, and (4) and (5) the respective monosulfates, is described.
Neil Kaplowitz, Norman B. Javitt
doaj   +1 more source

A plug-in electrophoresis microchip with PCB electrodes for contactless conductivity detection [PDF]

Royal Society Open Science, 2018
A plug-in electrophoresis microchip for large-scale use aimed at improving maintainability with low fabrication and maintenance costs is proposed in this paper.
Mingpeng Yang, Zhe Huang, Hui You
doaj   +1 more source

Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]

, 1982
The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő, Dudás, Brigitta, Erdei, Sára, Orosz, László   +3 more
core   +1 more source

Immobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis

Clinical Proteomics, 2011
Introduction The vast difference in the abundance of different proteins in biological samples limits the determination of the complete proteome of a cell type, requiring fractionation of proteins and peptides before MS analysis.
Seshi Beerelli, Raja Kumaraguru, Chandramouli KH   +2 more
doaj   +1 more source