Results 21 to 30 of about 510,638 (275)
Western blotting of native proteins from agarose gels
BioTechniques, 2022 We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma +10 more
doaj +1 more source
µTransparent insulating channels as components for miniaturized chemical separation Devices [PDF]
, 2000 Currently, miniaturized devices that apply electro osmotic pumping or electrophoretic separations are mostly constructed by etching small insulating channels for supply and separation on glass substrates.
Berg, A. van den +2 more
core +2 more sources
Micromachines, 2021 In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The dimensions of the device are 38 × 34 mm2 and it includes a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA ...
Jesús David Urbano-Gámez +2 more
doaj +1 more source
Integration of biological data resources using image object keying. [PDF]
, 2003 This paper proposes a novel concept of ‘image object keying'. The work builds on earlier research in this area and shows how the 3D structure of a protein can be retrieved interactively from a gel electrophoresis protein spot.
Khan, Nawaz +2 more
core +1 more source
Failure to detect "cap" structures in mitochondrial DNA-coded poly(A)-containing RNA from HeLa cells [PDF]
, 1978 The structure of the 5'-termini has been investigated in mitochondrial DNA- coded poly(A)-containing RNA from HeLa cells. For this purpose, mitochondrial RNA isolated from cells labeled for 3 hours with [32P]orthophosphate in the presence of 20 µg/ml ...
Amalric, François +3 more
core +1 more source
Agarose Gel Analysis of 15–40-kb PCR Amplimers
BioTechniques, 1996 Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to ...
Valerie Burland +2 more
doaj +1 more source
Protein electrophoretic migration data from custom and commercial gradient gels
Data in Brief, 2016 This paper presents data related to the article “A method for easily customizable gradient gel electrophoresis” (A.J. Miller, B. Roman, E.M. Norstrom, 2016) [1].
Andrew J. Miller +2 more
doaj +1 more source
20 Years of Fatty Acid Analysis by Capillary Electrophoresis
Molecules, 2014 A review taking into account the literature reports covering 20 years of fatty acid analysis by capillary electrophoresis is presented. This paper describes the evolution of fatty acid analysis using different CE modes such as capillary zone ...
Marcone Augusto Leal de Oliveira +6 more
doaj +1 more source
Novel microstructures and technologies applied in chemical analysis techniques [PDF]
, 1997 Novel glass and silicon microstructures and their application in chemical analysis are presented. The micro technologies comprise (deep) dry etching, thin layer growth and anodic bonding.
Burger, G.J. +5 more
core +3 more sources
Configurable on-Chip Gel Electrophoresis in Inkjet 3D Printed Microfluidic Modules
Proceedings, 2017 Inkjet 3D printing is with success used for preparation microfluidics devices as a microfluidic chips for DNA separation. Utilized this technic help us to prepare a chip in shorter time and cheaper, but length of microchannel are limited by possibility ...
Rafał Walczak +2 more
doaj +1 more source