Results 21 to 30 of about 319,758 (250)

A paper and plastic device for the combined isothermal amplification and lateral flow detection of Plasmodium DNA [PDF]

open access: yes, 2015
Background: Isothermal amplification techniques are emerging as a promising method for malaria diagnosis since they are capable of detecting extremely low concentrations of parasite target while mitigating the need for infrastructure and training ...
Richards-Kortum, Rebecca R.   +4 more
core   +1 more source

Agarose Gel Analysis of 15–40-kb PCR Amplimers

open access: yesBioTechniques, 1996
Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to ...
Valerie Burland   +2 more
doaj   +1 more source

Protein electrophoretic migration data from custom and commercial gradient gels

open access: yesData in Brief, 2016
This paper presents data related to the article “A method for easily customizable gradient gel electrophoresis” (A.J. Miller, B. Roman, E.M. Norstrom, 2016) [1].
Andrew J. Miller   +2 more
doaj   +1 more source

20 Years of Fatty Acid Analysis by Capillary Electrophoresis

open access: yesMolecules, 2014
A review taking into account the literature reports covering 20 years of fatty acid analysis by capillary electrophoresis is presented. This paper describes the evolution of fatty acid analysis using different CE modes such as capillary zone ...
Marcone Augusto Leal de Oliveira   +6 more
doaj   +1 more source

Configurable on-Chip Gel Electrophoresis in Inkjet 3D Printed Microfluidic Modules

open access: yesProceedings, 2017
Inkjet 3D printing is with success used for preparation microfluidics devices as a microfluidic chips for DNA separation. Utilized this technic help us to prepare a chip in shorter time and cheaper, but length of microchannel are limited by possibility ...
Rafał Walczak   +2 more
doaj   +1 more source

COVALENTLY BOUND PYRUVATE IN PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE FROM HORSE LIVER

open access: yes, 1987
Horse liver phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) incorporates nonexchangeable tritium from borotritide with a decrease of the activity. Substrate prevents both tritium incorporation and the decrease in activity.
Scandurra, R.   +10 more
core   +1 more source

Paper ionophoretic technique in the study of biologically important beryllium(II) / cobalt(II)-homoserine binary complexes in solution

open access: yesMacedonian Journal of Chemistry and Chemical Engineering, 2010
Quantitative indication of the process of forming a complex comes from the evaluation of the stability constants, which characterize the equilibria corresponding to the successive addition of ligands.
Brij Bhushan Tewari
doaj   +1 more source

The design of mechatronic system for gel electrophoresis [PDF]

open access: yesMATEC Web of Conferences, 2020
The paper present the methodology to design and implement a mechatronic system for gel electrophoresis. It describes the steps towards the development of a prototype and presents its functions. The new automated system is characterized by high precision,
Tsagaris Apostolos   +7 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Analysis of Dynamic Protein Expression Data

open access: yesRevstat Statistical Journal, 2005
Difference gel electrophoresis (DIGE) is the new gold standard analysing complex protein mixtures in proteomics. It is used for measuring the expression levels of proteins in different mixtures on the same two-dimensional electrophoresis (2-DE) gel.
Klaus Jung   +5 more
doaj   +1 more source

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