Results 21 to 30 of about 18,550,533 (314)

Protein concentration of synovial fluid in chronic rheumatoid arthritis. Estimation of protein in the synovial fluid of chronic rheumatoid arthritis by gel filtration and paper electrophoresis [PDF]

open access: yes, 1968
For the purpose to reveal the characteris6cs of the synovial fluid of the chronic rheumatoid arthritis the proteins of the synovial fluid and blood serum have been analysed by employing the methods of electrophoresis, gel filtration on Sephadex G-200 ...
Seno, Takashi
core   +1 more source

Quantitative analysis of unconjugated and conjugated bile acids in duodenal fluid by densitometry after paper electrophoresis

open access: yesJournal of Lipid Research, 1973
A new paper electrophoretic method for the separation of bile acids into five groups, (1) unconjugated, (2) glycine conjugates and (3) taurine conjugates, and (4) and (5) the respective monosulfates, is described.
Neil Kaplowitz, Norman B. Javitt
doaj   +1 more source

A plug-in electrophoresis microchip with PCB electrodes for contactless conductivity detection [PDF]

open access: yesRoyal Society Open Science, 2018
A plug-in electrophoresis microchip for large-scale use aimed at improving maintainability with low fabrication and maintenance costs is proposed in this paper.
Mingpeng Yang, Zhe Huang, Hui You
doaj   +1 more source

Power and limitations of electrophoretic separations in proteomics strategies [PDF]

open access: yes, 2009
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold   +225 more
core   +3 more sources

Immobilized pH gradient-driven paper-based IEF: a new method for fractionating complex peptide mixtures before MS analysis

open access: yesClinical Proteomics, 2011
Introduction The vast difference in the abundance of different proteins in biological samples limits the determination of the complete proteome of a cell type, requiring fractionation of proteins and peptides before MS analysis.
Seshi Beerelli   +2 more
doaj   +1 more source

Western blotting of native proteins from agarose gels

open access: yesBioTechniques, 2022
We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(N-morpholino)ethanesulfonic acid.
Chiaki Sakuma   +10 more
doaj   +1 more source

Integration of biological data resources using image object keying. [PDF]

open access: yes, 2003
This paper proposes a novel concept of ‘image object keying'. The work builds on earlier research in this area and shows how the 3D structure of a protein can be retrieved interactively from a gel electrophoresis protein spot.
Khan, Nawaz   +2 more
core   +1 more source

Semi-Automatic Lab-on-PCB System for Agarose Gel Preparation and Electrophoresis for Biomedical Applications

open access: yesMicromachines, 2021
In this paper, a prototype of a semi-automatic lab-on-PCB for agarose gel preparation and electrophoresis is developed. The dimensions of the device are 38 × 34 mm2 and it includes a conductivity sensor for detecting the TAE buffer (Tris-acetate-EDTA ...
Jesús David Urbano-Gámez   +2 more
doaj   +1 more source

Failure to detect "cap" structures in mitochondrial DNA-coded poly(A)-containing RNA from HeLa cells [PDF]

open access: yes, 1978
The structure of the 5'-termini has been investigated in mitochondrial DNA- coded poly(A)-containing RNA from HeLa cells. For this purpose, mitochondrial RNA isolated from cells labeled for 3 hours with [32P]orthophosphate in the presence of 20 µg/ml ...
Amalric, François   +3 more
core   +1 more source

Agarose Gel Analysis of 15–40-kb PCR Amplimers

open access: yesBioTechniques, 1996
Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel strength to ...
Valerie Burland   +2 more
doaj   +1 more source

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