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Overproduction of β-barrel outer membrane proteins in <i>Escherichia coli</i> BL21(DE3) induces hypervesiculation. [PDF]
Sahu S +5 more
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Overproduction and Characterization of Recombinant Soluble <i>Trypanosoma brucei</i> Phospholipase A<sub>2</sub>. [PDF]
Adepoju OA +9 more
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Cross-species rescue reveals sequence requirements for a rapidly evolving intrinsically disordered region. [PDF]
Park SO +8 more
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Affinity Electrophoresis of Proteins for Determination of Ligand Affinity and Exploration of Binding Sites. [PDF]
Masson P, Pashirova T.
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Hexameric-Based Hierarchy in the Sizes of a Cytolysin Pore-Forming Complex. [PDF]
Liu M +7 more
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A Modified FLT3 PCR Assay Using a TapeStation Readout. [PDF]
Blake EA, Ramineni M, Oltvai ZN.
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Polyacrylamide Gel Electrophoresis
Cold Spring Harbor Protocols, 2020Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation. Polyacrylamide gels have the following three major advantages over agarose gels: (1) Their resolving power is so great that they can separate molecules of
Michael R. Green, Joseph Sambrook
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Polyacrylamide Gel Electrophoresis
Science, 1971Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over ...
David Rodbard, Andreas Chrambach
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ELECTROPHORESIS OF HISTONES ON POLYACRYLAMIDE GELS
Canadian Journal of Biochemistry and Physiology, 1963Histories extracted from various organs of the rat have been fractionated by electrophoresis on Polyacrylamide gels. The most convenient reagents to form the gel were selected and the effects of varying concentrations of these reagents on the resolution of histones were investigated.
A. M. Marko, L. D. Johnson, A. Driedger
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