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Overproduction of β-barrel outer membrane proteins in <i>Escherichia coli</i> BL21(DE3) induces hypervesiculation. [PDF]

open access: yesExtracell Vesicles Circ Nucl Acids
Sahu S   +5 more
europepmc   +1 more source

Overproduction and Characterization of Recombinant Soluble <i>Trypanosoma brucei</i> Phospholipase A<sub>2</sub>. [PDF]

open access: yesEng Life Sci
Adepoju OA   +9 more
europepmc   +1 more source

Cross-species rescue reveals sequence requirements for a rapidly evolving intrinsically disordered region. [PDF]

open access: yesPLoS Biol
Park SO   +8 more
europepmc   +1 more source

Hexameric-Based Hierarchy in the Sizes of a Cytolysin Pore-Forming Complex. [PDF]

open access: yesBiomolecules
Liu M   +7 more
europepmc   +1 more source
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Polyacrylamide Gel Electrophoresis

Cold Spring Harbor Protocols, 2020
Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation. Polyacrylamide gels have the following three major advantages over agarose gels: (1) Their resolving power is so great that they can separate molecules of
Michael R. Green, Joseph Sambrook
openaire   +3 more sources

Polyacrylamide Gel Electrophoresis

Science, 1971
Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over ...
David Rodbard, Andreas Chrambach
openaire   +3 more sources

ELECTROPHORESIS OF HISTONES ON POLYACRYLAMIDE GELS

Canadian Journal of Biochemistry and Physiology, 1963
Histories extracted from various organs of the rat have been fractionated by electrophoresis on Polyacrylamide gels. The most convenient reagents to form the gel were selected and the effects of varying concentrations of these reagents on the resolution of histones were investigated.
A. M. Marko, L. D. Johnson, A. Driedger
openaire   +3 more sources

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