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Micro-Electrophoresis on Polyacrylamide Gels
1973Polyacrylamide gels were introduced in 1959 by Raymond and Weintraub, as supports for electrophoretic separations. The polyacrylamide gel is produced by polymerising acrylamide, with N,N-methylenebisacrylamide or ethylene diacrylate as the cross-linking component. Catalytic redox systems, which yield free radicals, are used to initate copolymerisation (
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SDS Polyacrylamide Gel Electrophoresis of Proteins
2003Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes.
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Discontinuous polyacrylamide gel electrophoresis of RNA
Analytical Biochemistry, 1972Abstract A discontinuous polyacrylamide gel system is described which allows the simultaneous resolution of 38S, 28S, 18S, 5S, and 4S RNA molecules from total RNA extracts on a single gel. The gels can be constructed easily and reproducibly without special apparatus. Also reported is the use of an octadecene solution to facilitate overlayering of low
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Neutral Polyacrylamide Gel Electrophoresis
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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SDS polyacrylamide gel electrophoresis
Trends in Biochemical Sciences, 2000openaire +2 more sources
Preparative electrophoresis on polyacrylamide gel
Analytical Biochemistry, 1964D, RACUSEN, N, CALVANICO
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