Results 281 to 290 of about 626,288 (358)

Micro-Electrophoresis on Polyacrylamide Gels

1973
Polyacrylamide gels were introduced in 1959 by Raymond and Weintraub, as supports for electrophoretic separations. The polyacrylamide gel is produced by polymerising acrylamide, with N,N-methylenebisacrylamide or ethylene diacrylate as the cross-linking component. Catalytic redox systems, which yield free radicals, are used to initate copolymerisation (
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High concentration polyacrylamide gel electrophoresis

Analytical Biochemistry, 1986
A simple technique is described for easy removal of high concentration polyacrylamide gels after electrophoresis from glass tubes without breaking them.
V S, Dhamankar, M D, Choudhury, H G, Vartak   +2 more
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‘Laterally aggregated’ polyacrylamide gels for electrophoresis

ELECTROPHORESIS, 1992
AbstractA new method is described for producing highly porous polyacrylamide matrices: polymerization in presence of a preformed hydrophilic polymer. If a standard mixture of monomers (e.g., 5%T, 4%C) is polymerized in presence of, e.g., polyethylene glycol (PEG) 10 kDa, lateral chain aggregation occurs, with formation of large pore sizes.
P G, Righetti, S, Caglio, M, Saracchi, S, Quaroni   +3 more
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SDS Polyacrylamide Gel Electrophoresis of Proteins

2003
Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes.
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SDS – polyacrylamide gel electrophoresis of lipopolysaccharides

Canadian Journal of Microbiology, 1975
Lipopolysaccharides (LPS) prepared from four different species of Neisseria have been separated by SDS – polyacrylamide gel electrophoresis. Each LPS possessed a characteristic mobility on gels. Examination of the effect of acrylamide concentration on migration illustrated that the basis of the separation was molecular size, and not intrinsic charge.
R R, Russell, K G, Johnson
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A miniaturized system for electrophoresis on polyacrylamide gels

Analytical Biochemistry, 1979
The design, construction, and use of a simple economical system for gel electrophoresis are described, along with effective procedures and the required materials. Examples of successful use of the system are presented.
Z I, Ogita, C L, Markert
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The fractionation of histones by electrophoresis in polyacrylamide gel

Analytical Biochemistry, 1965
Abstract Increased interest in histone fractionation is evidenced by the many published electrophoretic techniques using starch (1–3) and polyacrylamide (4–6) gels. Twenty-two components of calf thymus histone have been identified by Neelin and Neelin (7) with starch gel electrophoresis. Driedger et al.
G F, Vande Woude, F F, Davis
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Discontinuous polyacrylamide gel electrophoresis of RNA

Analytical Biochemistry, 1972
Abstract A discontinuous polyacrylamide gel system is described which allows the simultaneous resolution of 38S, 28S, 18S, 5S, and 4S RNA molecules from total RNA extracts on a single gel. The gels can be constructed easily and reproducibly without special apparatus. Also reported is the use of an octadecene solution to facilitate overlayering of low
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SDS-Polyacrylamide Gel Electrophoresis of Proteins

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThis protocol describes the separation of proteins by SDS-polyacrylamide gel electrophoresis. SDS is used with a reducing agent and heat to dissociate the proteins. SDS-polypeptide complexes form and migrate through the gels according to the size of the polypeptide.
Joseph, Sambrook, David W, Russell
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Separation of Colicins by Polyacrylamide Gel Electrophoresis

Journal of Applied Bacteriology, 1972
S ummary : Colicins can be separated by polyacrylamide gel electrophoresis, so allowing multiple colicinogeny to be detected with a universal indicator strain.
K G, Hardy, H C, Neimark
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