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SIMPLIFIED “DISC” (POLYACRYLAMIDE GEL) ELECTROPHORESIS*

Annals of the New York Academy of Sciences, 1964
SummaryCross‐linked homogeneous 5 per cent polyacrylamide gel in rod form is a convenient medium for sharp analytical electrophoretic separation in microgram quantities of most serum proteins and of many biological ampholytes.
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Polyacrylamide Temperature Gradient Gel Electrophoresis

2013
Temperature Gradient Gel Electrophoresis (TGGE) is a form of electrophoresis in which temperature gradient is used to denature molecules as they move through either acrylamide or agarose gel. TGGE can be applied to analyze DNA, RNA, protein-DNA complexes, and, less commonly, proteins.
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Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels

2003
For the majority of purposes, such as restriction mapping and sizing of cloned fragments, electrophoresis of DNA in horizontal agarose gels is perfectly adequate. As the size of the fragments of interest decreases to below 1000 base pairs (bp), however, the resolution deteriorates significantly, and an alternative is required.
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Preparative electrophoresis on polyacrylamide gel

Analytical Biochemistry, 1964
D, RACUSEN, N, CALVANICO
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One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE).

Methods in Enzymology, 2014
J. Brunelle, R. Green
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Neutral Polyacrylamide Gel Electrophoresis

Cold Spring Harbor Protocols, 2006
Joseph, Sambrook, David W, Russell
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Gradient SDS Polyacrylamide Gel Electrophoresis

2003
The preparation of fixed-concentration polyacrylamide gels has been described in Chapter 6. However, the use of polyacrylamide gels that have a gradient of increasing acrylamide concentration (and hence decreasing pore size) can sometimes have advantages over fixed-concentration acrylamide gels. During electrophoresis in gradient gels, proteins migrate
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