Topological Patterns in Two-dimensional Gel Electrophoresis of DNA Knots [PDF]
Gel electrophoresis is a powerful experimental method to probe the topology of DNA and other biopolymers. While there is a large body of experimental work which allows us to accurately separate different topoisomers of a molecule, a full theoretical understanding of these experiments has not yet been achieved.
arxiv +1 more source
Characterization of the Lipopolysaccharide from a \u3cem\u3eRhizobium phaseoli\u3c/em\u3e Mutant that is Defective in Infection Thread Development [PDF]
The lipopolysaccharide (LPS) from a Rhizobium phaseoli mutant, CE109, was isolated and compared with that of its wild-type parent, CE3. A previous report has shown that the mutant is defective in infection thread development, and sodium dodecyl sulfate ...
Carlson, Russell W.+4 more
core +1 more source
The electrophoresis of histones in polyacrylamide gel and their quantitative determination.
1. A new method has been devised for the separation of the histone fractions of calf thymus by electrophoresis in polyacrylamide gel at pH2.4. 2. The fractions have been characterized by their relative mobilities with respect to a marker protein, bovine ...
E. W. Johns
semanticscholar +1 more source
INTRODUCTION: The aim of the present study was to evaluate the presence of arboviruses from the Flavivirus genus in asymptomatic free-living non-human primates (NHPs) living in close contact with humans and vectors in the States of Paraná and Mato Grosso
Tatiana Carneiro da Rocha+7 more
doaj +1 more source
Guidelines for reporting the use of gel electrophoresis in proteomics [PDF]
the MIAPE Gel Electrophoresis (MIAPE-GE) guidelines specify the minimum information that should be provided when reporting the use of n-dimensional gel electrophoresis in a proteomics experiment. Developed through a joint effort between the gel-based analysis working group of the Human Proteome Organisation's Proteomics Standards Initiative (HUPO-PSI ...
arxiv +1 more source
Development of a Procedure for Analysis of High Density Lipoprotein Subclasses [PDF]
Human serum high density lipoprotein subclasses, HDL2 and HDL3 , were isolated by preparative salt density gradient ultracentrifugation and further analyzed by electrophoresis on a 4 to 15% concentration gradient polyacrylamide gel. The separation of the
Chin, Chan
core +2 more sources
Residual casein fractions in ripened cheese determined by polyacrylamide-gel electrophoresis.
Polyacrylamide-gel electrophoresis (PAE) of ripened-cheese caseins provides an ideal means of studying cheese-ripening by detecting small changes in the specific casein fractions.
R. Ledford, A. O'sullivan, K. Nath
semanticscholar +1 more source
Silver-staining of proteins in polyacrylamide gels: a general overview [PDF]
On the basis of the physico-chemical principles underlying silver-staining of proteins, which are recalled in this paper, several methods of silver-staining of proteins after SDS electrophoresis in polyacrylamide gels or isoelectric focusing were tested.
arxiv
Biologic activity in a fragment of recombinant human interferon α [PDF]
To attempt to locate functionally important regions of the interferon (IFN) molecule, recombinant human IFN-α2 was subjected to proteolytic digestion. The bacterial proteinase thermolysin produced two major complementary fragments, HuIFN-α2-(1-110) and ...
Ackerman, Samuel K.+4 more
core
Membrane proteins and proteomics: Love is possible, but so difficult [PDF]
Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi+103 more
core +4 more sources