Results 111 to 120 of about 1,139,090 (295)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Ephemeropteran and Trichopteran Assemblages Vary Across a Subtropical Rainforest Altitudinal Gradient: Useful Indicators for Climate Change

Ecology and Evolution
The subtropical rainforests of eastern Australia are expected to be greatly affected by climate change, with several studies predicting an upward shift in elevational distribution for many groups of fauna and flora.
D. Pagotto, C. Burwell, K. Turlington, F. Sheldon   +3 more
doaj   +1 more source

Humic Acid Composition and Characteristics of Soil Organic Matter in Relation to the Elevation Gradient of Moso Bamboo Plantations.

PLoS ONE, 2016
Studying the influence of climatic and/or site-specific factors on soil organic matter (SOM) along an elevation gradient is important for understanding the response of SOM to global warming.
Hsueh-Ching Wang, Chiao-Ying Chou, Chyi-Rong Chiou, Guanglong Tian, Chih-Yu Chiu   +4 more
doaj   +1 more source

Evaluating the involvement of autolysosomes in the nuclear translocation of fluorescent proteins

FEBS Open Bio, EarlyView.
Endogenously expressed fluorescent proteins can be degraded by autophagy and transported to cell nuclei via the nuclear pore complex. But in some cell lines, for example, HeLa cells which are positive for immunoreactivity of a receptor ligand, such as UCN I, in cell nuclei, fusion of autolysosome with the nuclear envelope is involved in the nuclear ...
Keiichi Ikeda
wiley   +1 more source

Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region

FEBS Open Bio, EarlyView.
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson, Anja H. Schiemann, Evelyn Sattlegger   +2 more
wiley   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

FEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang, Mingqiang Deng, Dongmei He, Diqin Lai, Dongjun Liu, Dengyong Zhu, Zhenhua Zhang, Peng Tang, Chuanman Zhou, Menghui Yin, Xichen Bao   +10 more
wiley   +1 more source

Plant species composition under reciprocal transplantion along elevation gradient

, 2019
The data file is how response of plant species to warming and cooling when reciprocal transplantion along 3200-3800 m elevation gradient form 2007 to 2015 on the Tibetan ...
Caiyun Luo (562630), Meng, Fandong, Huakun Zhou (5229731), Liu, Peipei, Du, Mingyuan, Bowen Li (200859), Peipei Liu (339689), Wangwang Lv (7072004), Li, Bowen, Yaoming Li (325714), Lili Jiang (65114), Dorji, Tsechoe, Tsechoe Dorji (7058276), Lirong Zhang (391975), Amy Iler (6451214), Iler, Amy, Yanfen Wang (104864), Zhezhen Wang (7072010), Luo, Caiyun, Zhenhua Zhang (562631), Li, Yingnian, Shiping Wang (13805), Jiang, Lili, Wang, Yanfen, Mingyuan Du (566715), Wang, Shiping, Li, Yaoming, Zhao, Xinquan, Fandong Meng (591766), Duan, Jichuang, Zhou, Huakun, Du, Rui, Lv, Wangwang, Qi Wang (22418), Jichuang Duan (2924817), Suonan, Ji, Shilong Piao (527767), Yingnian Li (572547), Zhang, Lirong, Ji Suonan (7072007), Xinquan Zhao (175113), Rui Du (317749), Zhang, Zhenhua, Wang, Zhezhen, Wang, Qi, Piao, Shilong   +45 more
core   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

) varies significantly along an elevation gradient

, 2012
International audienceLittle is known about the potential effect of climate warming on phyllosphere fungi, despite their important impact on the dynamics and diversity of plant communities. The structure of phyllosphere fungal assemblages along elevation
Tristan Cordier, Desprez Loustau, Marie Laure,, Vacher, Corinne, C., Corinne Vacher, Cordier, Tristan, Xavier Capdevielle, DESPREZ LOUSTAU, Marie Laure, Fabreguettes, Olivier, Cécile Robin, Olivier Fabreguettes, Marie‐Laure Desprez‐Loustau, VACHER, Corinne, Robin, Cécile,, Desprez Loustau, Marie Laure, M. L., Capdevielle, Xavier, ROBIN, Cécile, Vacher, Corinne,, Robin, Cécile, C.   +17 more
core   +1 more source

Four simplified gradient elasticity models for the simulation of dispersive wave propagation [PDF]

, 2008
Gradient elasticity theories can be used to simulate dispersive wave propagation as it occurs in heterogeneous materials. Compared to the second-order partial differential equations of classical elasticity, in its most general format gradient elasticity ...
Pichugin, AV, Bennett, T, Metrikine, A., Askes, H., Bennett, T., Pichugin, A., Metrikine, AV, Askes, H   +7 more
core   +1 more source