Results 1 to 10 of about 548,250 (354)

Enhancing therapeutic antibody production through amino acid-induced pH shift in Protein A affinity chromatography [PDF]

Frontiers in Bioengineering and Biotechnology
Protein aggregation, denaturation, and loss of potency often occur during Protein A chromatography due to the harsh acidic conditions required for antibody elution.
Senzhu Lin, Yue Wang
doaj   +2 more sources

Cutting through the weeds: Evaluation of a novel adsorption with crossmatch cells and elution protocol to sharpen HLA antibody identification by the single antigen bead assay

Frontiers in Genetics, 2022
The single antigen bead (SAB) assay is the most used test for the identification of HLA specific antibodies pre- and post-transplant. Nevertheless, detection of spurious reactivities remains a recognized assay limitation.
Robert S. Liwski, Sandra Tafulo, Robert Carroll, Robert Carroll, James H. Lan, Anna L. Greenshields   +5 more
doaj   +1 more source

Dried urine spot method for detection of Schistosoma mansoni circulating cathodic antigen in resource-limited settings: a proof of concept study

Frontiers in Immunology, 2023
BackgroundAmong the challenges in schistosomiasis surveillance and mapping surveys is the lack of a sensitive diagnostic method especially in low transmission setting.
Abdallah Zacharia, Twilumba Makene, Clemence Kinabo, George Ogweno, Faraja Lyamuya, Billy Ngasala   +5 more
doaj   +1 more source

“Auto-anti-A1” in a healthy young blood donor: A rare cause of ABO discrepancy

Asian Journal of Transfusion Science, 2021
”Auto-anti-A1” has been sparsely discussed in the literature. Only few workers in the past depicted such antibody in transfused and nontransfused patients.
Sudipta Sekhar Das, Rathindra Nath Biswas, Mahammad Safi   +2 more
doaj   +1 more source

A case report of multiple Rh alloantibodies in a pregnant female: Resolution by sequential adsorption

Asian Journal of Transfusion Science, 2021
Anti-G antibody mimics the reactivity pattern of coexistent anti-D and anti-C. Differentiating between the two is significant in antenatal females where the decision to administer RhD prophylaxis is based on the presence or absence of anti-D antibody ...
Divya Setya, Aseem K Tiwari, Dinesh Arora, Swati Pabbi Mehta, Geet Aggarwal   +4 more
doaj   +1 more source

Comparative Evaluation of Two Antifungal Agents Incorporated in Auto Polymerising Denture Base Resin, Heat Polymerising Denture Base Resin and Permanent Silicone Soft Liner-An In Vitro Study [PDF]

Journal of Clinical and Diagnostic Research, 2019
Introduction: Denture-induced candidiasis is a common disease in elderly denture wearers with Candida albicans as the principal causative agent. The problem is aggravated for elderly patients with limited motor skills who fail to follow a strict ...
Swapnil Chincholikar, J Sridevi, N Kalavathy, Sudhanshu Singh, Anmol Kapoor, Sonal Saumya   +5 more
doaj   +1 more source

Determination of the Elution Capacity of Dalbavancin in Bone Cements: New Alternative for the Treatment of Biofilm-Related Peri-Prosthetic Joint Infections Based on an In Vitro Study

Antibiotics, 2022
Antibiotic-loaded bone cement is the most widely used approach for the treatment of biofilm-induced septic sequelae in orthopedic surgery. Dalbavancin is a lipoglycopeptide that acts against Gram-positive bacteria and has a long half-life, so we aimed to
Mar Sánchez-Somolinos, Marta Díaz-Navarro, Antonio Benjumea, Marta Tormo, José Matas, Javier Vaquero, Patricia Muñoz, Pablo Sanz-Ruíz, María Guembe   +8 more
doaj   +1 more source

Release of Monomers from Dental Composite Materials into Saliva and the Possibility of Reducing the Toxic Risk for the Patient

Medicina, 2023
Background and Objectives: The objective of this study was (1) to measure the amount of monomers released into the saliva depending on the time elapsed after the hardening of the composite and on the type of monomer used; and (2) with the prolongation of
Soňa Tkáčiková, Ján Sabo
doaj   +1 more source

Rapid Assessment of Di(2-ethylhexyl) Phthalate Migration from Consumer PVC Products

Toxics, 2023
Poly(vinyl chloride) (PVC) is widely used to produce various consumer goods, including food packaging, toys for children, building materials, and cosmetic products.
Jiwon An, Hyun-Ho Roh, Haeyoon Jeong, Kuen-Yong Lee, Taiyoun Rhim   +4 more
doaj   +1 more source

Effect of Melt Cooling Condition on As and Pb Elution Properties in Copper Slag

Journal of MMIJ, 2022
Copper slag (CS) granules received from a Japanese smelter were melted using an alumina crucible under Ar gas flow in an electric furnace. The melt was removed from the furnace and cooled slowly with various holding times in air and then water-quenched ...
Chika ONISHI, Shuto YOSHIFUKU, Hiromichi TAKEBE   +2 more
doaj   +1 more source