Results 111 to 120 of about 637,249 (369)

CDK11 inhibition induces cytoplasmic p21WAF1 splice variant by p53 stabilisation and SF3B1 inactivation

Molecular Oncology, EarlyView.
CDK11 inhibition stabilises the tumour suppressor p53 and triggers the production of an alternative p21WAF1 splice variant p21L, through the inactivation of the spliceosomal protein SF3B1. Unlike the canonical p21WAF1 protein, p21L is localised in the cytoplasm and has reduced cell cycle‐blocking activity.
Radovan Krejcir, Lukasz Arcimowicz, Lucia Martinkova, Vaclav Hrabal, Filip Zavadil Kokas, Tomas Henek, Martina Kucerikova, Ondrej Bonczek, Pavlina Zatloukalova, Lenka Hernychova, Philip J. Coates, Borivoj Vojtesek, David P. Lane   +12 more
wiley   +1 more source

Improved Native Isolation of Endogenous Protein A-Tagged Protein Complexes

BioTechniques, 2013
Here we report a modified peptide reagent useful for the rapid, native elution of protein complexes containing a Protein-A-tagged component. We tested this reagent for the elution of tagged endogenous protein complexes from yeast (Nup53p/Nup170p dimer ...
John LaCava, Nagarajan Chandramouli, Hua Jiang, Michael P. Rout   +3 more
doaj   +1 more source

Methionine 129 variant of human prion protein oligomerizes more rapidly than the valine 129 variant - Implications for disease susceptibility to Creutzfeldt-Jakob disease [PDF]

, 2004
The human PrP gene (PRNP) has two common alleles that encode either methionine or valine at codon 129. This polymorphism modulates disease susceptibility and phenotype of human transmissible spongiform encyphalopathies, but the molecular mechanism by ...
Abdessamad Tahiri-Alaoui, Andrew C. Gill, Petra Disterer, William James, Prusiner, Weissmann, Will, Bruce, Hill, Prusiner, Prusiner, Collinge, Windl, Palmer, Brown, Baker, Cervenakova, Parchi, Hill, Collinge, Hauw, Goldfarb, Goldfarb, Dlouhy, Dermaut, Croes, Liemann, Riek, Alonso, Baskakov, Jackson, Baskakov, Gill, Lu, Lu, Landon, Safar, Bessen, Zahn, Riek, Nguyen, Tahiri-Alaoui, Santini, Wong, Wildegger, Matouschek, Vendruscolo, Kim, Fasman, Baskakov, Qin, Oesch, Conway, Sayer, Rhie, Schatzl, Mead   +56 more
core   +1 more source

Dual targeting of RET and SRC synergizes in RET fusion‐positive cancer cells

Molecular Oncology, EarlyView.
Despite the strong activity of selective RET tyrosine kinase inhibitors (TKIs), resistance of RET fusion‐positive (RET+) lung cancer and thyroid cancer frequently occurs and is mainly driven by RET‐independent bypass mechanisms. Son et al. show that SRC TKIs significantly inhibit PAK and AKT survival signaling and enhance the efficacy of RET TKIs in ...
Juhyeon Son, Lily L. Remsing Rix, Bin Fang, Eric A. Welsh, Nicole V. Bremer, Valentina Foglizzo, Paola Roa, Nickole Sigcha‐Coello, Yi Liao, Eric B. Haura, Alexander Drilon, John M. Koomen, Emiliano Cocco, Uwe Rix   +13 more
wiley   +1 more source

The development of a purification procedure for Peptide:n-Glycosidase A from Prunus amygdalus : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University [PDF]

, 1997
Peptide-N4-acetyl-β-glucosaminyl) asparagine amidases cleave the amide bond between N-linked glycans at N-acetylglucosamine and asparagine, liberating intact oligosaccharide chains from glycoproteins. Although PNGase A is commonly used by glycobiologists
Diepen, Angela Corinne van
core  

Valorization of keratin biofibers for removing heavy metals from aqueous solutions [PDF]

, 2018
Four common waste keratin biofibers (human hair, dog hair, chicken feathers, and degreased wool) have been used as biosorbents for the removal of heavy metal ions from aqueous solutions. Different parameters of the biosorption processes were optimized in
Carrillo Navarrete, Fernando, López Mesas, Montserrat, Palet, Cristina, Zhang, Helan   +3 more
core   +2 more sources

Molecular characterisation of human penile carcinoma and generation of paired epithelial primary cell lines

Molecular Oncology, EarlyView.
Generation of two normal and tumour (cancerous) paired human cell lines using an established tissue culture technique and their characterisation is described. Cell lines were characterised at cellular, protein, chromosome and gene expression levels and for HPV status.
Simon Broad, Mahmood Hachim, Tom Bertin, Karolina Penderecka, Rifat Hamoudi, Saif Khan, Rui Henrique, Natalie Bergmoser, Manit Arya, Kalle Sipila, Matteo Vietri Rudan, Asif Muneer, Aamir Ahmed   +12 more
wiley   +1 more source

A Rare Case of Pregnancy-induced Anti-D, Anti-C, and Anti-G Antibodies

Global Journal of Transfusion Medicine
A 39-year-old female with breast carcinoma presented with complaints of menorrhagia and weakness. At current admission, her hemoglobin was 6.2 g/dL, and a requisition for packed red blood cells (PRBCs) was sent to the blood center.
Ayesha Sinha, Najla Haneefa Basheela, Durba Biswas, Debapriya Basu, Suvro Sankha Datta   +4 more
doaj   +1 more source

Studies on methodology in dietary fibre analysis : a neutral detergent fibre method using glucoamylase : a thesis presented in partial fulfilment of the requirements for the degree of Master of Philosophy in Food Technology at Massey University [PDF]

, 1979
The dietary fibre content of foods is conveniently and rapidly determined by the neutral and acid detergent methods devised originally by Van Soest and associates.
Sills, Victor Ernest
core  

Pitfall in the high-throughput quantification of whole blood cyclosporin A using liquid chromatography-tandem mass spectrometry [PDF]

, 2005
In a growing number of laboratories the technique of liquid chromatography-tandem mass spectrometry is used for the quantification of cyclosporin A in whole blood, employing cyclosporin D as the internal standard. Cyclosporin A is extensively metabolized
Spöhrer, Ute, Vogeser, Michael
core   +1 more source