Results 111 to 120 of about 548,250 (354)

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Weak anion-exchange hypercrosslinked sorbent in on-line solid-phase extraction-liquid chromatography coupling to achieve automated determination with an effective clean-up [PDF]

, 2010
A mixed-mode polymeric sorbent was on-line coupled to liquid chromatography (LC) for the first time and applied it to the selective solid-phase extract a group of pharmaceuticals in complex environmental water samples. The mixed-mode polymeric sorbent is
Borrull, F., Cormack, P.A.G., Fontanals, N., Marce, R.M., Sherrington, D.C.   +4 more
core   +1 more source

A Rare Case of Pregnancy-induced Anti-D, Anti-C, and Anti-G Antibodies

Global Journal of Transfusion Medicine
A 39-year-old female with breast carcinoma presented with complaints of menorrhagia and weakness. At current admission, her hemoglobin was 6.2 g/dL, and a requisition for packed red blood cells (PRBCs) was sent to the blood center.
Ayesha Sinha, Najla Haneefa Basheela, Durba Biswas, Debapriya Basu, Suvro Sankha Datta   +4 more
doaj   +1 more source

A Suppression Method for Elution of F−, [B(OH)4]−, AsO43−, and CrO42− from Industrial Wastes Using Some Inhibitors and Crushed Stone Powder

Technologies, 2018
The disposal and the reuse of industrial wastes have become increasingly difficult due to the elution of hazardous anions, such as F−, [B(OH)4]−, AsO43−, and CrO42−.
Xiaoxu Kuang, Atsuki Sentoku, Atsushi Sasaki, Masatoshi Endo   +3 more
doaj   +1 more source

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

FEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa, Yasushi Shigeri, Shingo Fujisaki   +2 more
wiley   +1 more source

Designer Reagents for Mass Spectrometry-Based Proteomics: Clickable Cross-Linkers for Elucidation of Protein Structures and Interactions [PDF]

, 2012
We present novel homobifunctional amine-reactive clickable cross-linkers (CXLs) for investigation of three-dimensional protein structures and protein–protein interactions (PPIs).
Agnew, Heather D., Beauchamp, J. L., Czerwieniec, Gregg, Deshaies, Raymond J., Graham, Robert L. J., Heath, James R., Hess, Sonja, Lee, J. Eugene, Loo, Joseph A., Smith, Geoffrey T., Sohn, Chang Ho, Sweredoski, Michael J.   +11 more
core   +2 more sources

Sammenligning av metoder for eluering av IgG-antistoff bundet til erytrocytter

Bioingeniøren
Background: IgG antibodies bound to erythrocytes may be involved in various disease processes. Identification of these antibodies and antigen typing of patients with a positive direct antiglobulin (DAT) test is relevant both for transfusion therapy and ...
Elin Hallheim Reiersøl, Thomas E. Hundhausen   +1 more
doaj  

Removal of antibodies from red cells: Comparison of three elution methods

Asian Journal of Transfusion Science, 2013
Background: Direct antiglobulin test (DAT) is the most common test done in immunohematology lab, which detects immunoglobulin and fragments of complement attached to the red blood cells. These coated red blood cells are difficult to accurately phenotype,
Rahul Katharia, Rajendra K Chaudhary
doaj   +1 more source

Interaction of class III cellobiose dehydrogenase with lytic polysaccharide monooxygenase

FEBS Open Bio, EarlyView.
The activity of lytic polysaccharide monooxygenase (LPMO) is supported by its auxiliary enzyme cellobiose dehydrogenase (CDH). The catalytic activity of both enzymes is coupled by electron transfer and a cyclic cascade generating substrates for both enzymes – hydrogen peroxide for LPMO and oxidized and non‐oxidized cellobiose and cello‐oligosaccharide ...
Angela Giorgianni, Florian Csarman, Peicheng Sun, Mirjam Kabel, Roland Ludwig   +4 more
wiley   +1 more source

Mechanism of the diuretic activity of Descurainia sophia seed

Bangladesh Journal of Pharmacology, 2018
The diuretic activity of the decoction and the chemical fractions of Descurainia sophia was studied with underlying mechanisms in rats. Rat metabolic cage method was used to study the diuretic activity of the decoction and the chemical fractions of D ...
Mengnan Zeng, Li Zhang, Miao Li, Beibei Zhang, Weisheng Feng, Haixue Kuang, Xiaoke Zheng   +6 more
doaj   +1 more source