Results 151 to 160 of about 635,014 (389)

Overexpression of CDT1 inhibits cell cycle progression at S phase by interacting with the mini‐chromosome maintenance complex and causes DNA damage

FEBS Open Bio, EarlyView.
CDT1 is an essential protein for DNA replication licensing that loads the MCM complex, the eukaryotic replicative DNA helicase, onto replication origins. Overexpression of CDT1 induces cell cycle arrest at the S phase. Here we showed CDT1 inhibits the progression of replication forks by interacting with the MCM complex, leading to the stalling and ...
Takashi Tsuyama, Nonoka Takayama, Rina Tanaka, Yuuki Arai, Yohko Yamaguchi, Yuko Nawata, Yutaro Azuma, Shusuke Tada   +7 more
wiley   +1 more source

In vitro Study of Elution Characteristics of Antiviral Drugs Immersed in Bone Cement

Туберкулез и болезни лёгких
The objective: to assess elution characteristics of certain antiviral drugs immersed in bone cement samples and placed in a liquid medium to determine the fundamental possibility of using such systems.Subjects and Methods.
E. O. Peretsmanas, A. A. Artyukhov, I. A. Gerasimov, V. S. Zubikov, T. E. Tyulkova, Ya. A. Rukin   +5 more
doaj   +1 more source

Methionine 129 variant of human prion protein oligomerizes more rapidly than the valine 129 variant - Implications for disease susceptibility to Creutzfeldt-Jakob disease [PDF]

, 2004
The human PrP gene (PRNP) has two common alleles that encode either methionine or valine at codon 129. This polymorphism modulates disease susceptibility and phenotype of human transmissible spongiform encyphalopathies, but the molecular mechanism by ...
Abdessamad Tahiri-Alaoui, Andrew C. Gill, Petra Disterer, William James, Prusiner, Weissmann, Will, Bruce, Hill, Prusiner, Prusiner, Collinge, Windl, Palmer, Brown, Baker, Cervenakova, Parchi, Hill, Collinge, Hauw, Goldfarb, Goldfarb, Dlouhy, Dermaut, Croes, Liemann, Riek, Alonso, Baskakov, Jackson, Baskakov, Gill, Lu, Lu, Landon, Safar, Bessen, Zahn, Riek, Nguyen, Tahiri-Alaoui, Santini, Wong, Wildegger, Matouschek, Vendruscolo, Kim, Fasman, Baskakov, Qin, Oesch, Conway, Sayer, Rhie, Schatzl, Mead   +56 more
core   +1 more source

A general model for analysis of linear and hyperbolic enzyme inhibition mechanisms

FEBS Open Bio, EarlyView.
We developed a general enzyme kinetic model that integrates these six basic inhibition mechanism onto a single one. From this model, we deduced a general enzyme kinetic equation that through modulation of simple parameters, γ (the relative inhibitor affinity for two binding sites) and β (the reactivity of the enzyme–substrate–inhibitor complex), is ...
Rafael S. Chagas, Sandro R. Marana
wiley   +1 more source

Enhancing therapeutic antibody production through amino acid-induced pH shift in Protein A affinity chromatography

Frontiers in Bioengineering and Biotechnology
Protein aggregation, denaturation, and loss of potency often occur during Protein A chromatography due to the harsh acidic conditions required for antibody elution.
Senzhu Lin, Yue Wang
doaj   +1 more source

HSP70 governs permeability and mechanotransduction in primary human endothelial cells

FEBS Open Bio, EarlyView.
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez, Everton G. Melo, Isadora C. B. Pavan, Percíllia V. S. Oliveira, Luiza B. C. T. Coimbra, Thaís L. S. Araujo   +5 more
wiley   +1 more source

Comparison of the efficacy of heat and acid elution methods for IgG anti-M and anti-Ku

Zhongguo shuxue zazhi
[Objective] To compare the efficacy of heat and acid elution methods for IgG anti-M and anti-Ku. [Methods] Ten samples with IgG anti-M and two samples with IgG anti-Ku were selected and standardized to a titer of 64.
ZENG Qunjuan, KANG Huaiying, XIANG Dong, SHEN Wei, QIAN Chengrui, WANG Zhongying, GONG Guoqin   +6 more
doaj   +1 more source

Mechanism of the diuretic activity of Descurainia sophia seed

Bangladesh Journal of Pharmacology, 2018
The diuretic activity of the decoction and the chemical fractions of Descurainia sophia was studied with underlying mechanisms in rats. Rat metabolic cage method was used to study the diuretic activity of the decoction and the chemical fractions of D ...
Mengnan Zeng, Li Zhang, Miao Li, Beibei Zhang, Weisheng Feng, Haixue Kuang, Xiaoke Zheng   +6 more
doaj   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source