Results 151 to 160 of about 637,249 (369)

Impact of extraction and elution media on non-size effects in size exclusion chromatography of proteins [PDF]

open access: green, 2015
Marlies A. Lambrecht   +3 more
openalex   +1 more source

Engineering tandem VHHs to target different epitopes to enhance antibody‐dependent cell‐mediated cytotoxicity

open access: yesFEBS Open Bio, EarlyView.
Tandem VHH targeting distinct EGFR epitopes were engineered into a monovalent bispecific antibody (7D12‐EGA1‐Fc) with more potent ADCC without increasing affinity to EGFR. Structural modeling of 7D12‐EGA1‐Fc showed cross‐linking of separate EGFR domains to enhance CD16a engagement on NK cells.
Yuqiang Xu   +5 more
wiley   +1 more source

Local Alendronic Acid Elution Increases Net Periimplant Bone Formation: A Micro-CT Analysis [PDF]

open access: bronze, 2013
J Bobyn   +5 more
openalex   +1 more source

FGFR Like1 drives esophageal cancer progression via EMT, PI3K/Akt, and notch signalling: insights from clinical data and next‐generation sequencing analysis

open access: yesFEBS Open Bio, EarlyView.
Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava   +3 more
wiley   +1 more source

Gel filtration chromatography [PDF]

open access: yes, 2011
Gel-filtration chromatography is a popular and versatile technique that permits the effective separation of proteins and other biological molecules in high yield. Here, the basis of the method is described and typical matrix types are contrasted.
Cummins, Phil   +2 more
core  

Optimization of automated radiosynthesis of gallium-68-labeled PSMA11 with two 68Ge/68Ga generators: fractional elution or prepurification? [PDF]

open access: green, 2023
Flore Durieux   +6 more
openalex   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Comparative evaluation of various disc elution methods for the detection of colistin-resistant gram-negative bacteria

open access: yesOpen Medicine
The current study was designed to determine the performance of colistin broth disc elution (CBDE), colistin broth micro elution (CBME), and microplate elution (MPE) methods with the broth microdilution (BMD) reference method.
Chauhan Shubham   +7 more
doaj   +1 more source

Enhanced 3-epi-25-hydroxyvitamin D3 signal leads to overestimation of its concentration and amplifies interference in 25-hydroxyvitamin D LC-MS/MS assays [PDF]

open access: yes, 2014
Background 3-epi-25-hydroxyvitamin D3 (3-epi-25OHD3) interferes in most liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays for 25-hydroxyvitamin D (25OHD).
Dawnay, A, Flynn, N, Lam, F
core  

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