Results 81 to 90 of about 637,249 (369)
Elution of Artificial Sputum from Swab by Rotating Magnetic Field-Induced Mechanical Impingement
Applied Sciences, 2017 Cotton-tipped applicator swabs are used as a collection device for many biological samples and its complete elution is a desired step for clinical and forensic diagnostics.
Shubham Banik +2 more
doaj +1 more source
Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE [PDF]
, 2010 A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC ...
Bernd Kastenholz, David E. Garfin
core +2 more sources
FEBS Letters, EarlyView.At low cell density, SETDB1 and YAP1 accumulate in the nucleus. As cell density increases, the Hippo pathway is gradually activated, and SETDB1 is associated with increased YAP1 phosphorylation. At high cell density, phosphorylated YAP1 is sequestered in the cytoplasm, while SETDB1 becomes polyubiquitinated and degraded by the ubiquitin–proteasome ...
Jaemin Eom +3 more
wiley +1 more source
Improved elution conditions for native co-immunoprecipitation. [PDF]
PLoS ONE, 2011 Native immunoprecipitation followed by protein A-mediated recovery of the immuno-complex is a powerful tool to study protein-protein interactions. A limitation of this technique is the concomitant recovery of large amounts of immunoglobulin, which ...
Robin Antrobus, Georg H H Borner
doaj +1 more source
Molecular Oncology, EarlyView.AZD9291 has shown promise in targeted cancer therapy but is limited by resistance. In this study, we employed metabolic labeling and LC–MS/MS to profile time‐resolved nascent protein perturbations, allowing dynamic tracking of drug‐responsive proteins. We demonstrated that increased NNMT expression is associated with drug resistance, highlighting NNMT ...
Zhanwu Hou +5 more
wiley +1 more source
“Block D” Phenomenon in Hemolytic Disease of Fetus and Newborn: The Immunohematological Implications
Global Journal of Transfusion MedicineBackground and Objectives: “Block D” in hemolytic disease of the fetus and newborn (HDFN) is quite rare and should be evaluated meticulously. We analyzed “Block D” phenomenon in neonates with HDFN due to rhesus (Rh) incompatibilities.
Sourav Mukherjee +2 more
doaj +1 more source
Molecular Oncology, EarlyView.PARP inhibitors are used to treat a small subset of prostate cancer patients. These studies reveal that PARP1 activity and expression are different between European American and African American prostate cancer tissue samples. Additionally, different PARP inhibitors cause unique and overlapping transcriptional changes, notably, p53 pathway upregulation.
Moriah L. Cunningham +21 more
wiley +1 more source
BioTechniques, 2020 The use of avidin or streptavidin in the purification of biotinylated proteins has been highly restricted due to the harsh and denaturing elution conditions.
Kui Lin +5 more
doaj +1 more source
Synthesis of polyformals [PDF]
, 1981 Formals of CH2OH(CHOH) sub n CH2OH polyols (n = 2 to 4) are prepared in less than 15 minutes by heating to about 125 C, a mixture of e.g. sorbitol and paraformaldehyde in slight excess (5 to 10%), in the presence of e.g.
Poshkus, A. C.
core +1 more source
Association between cholesterol-phospholipid vesicles and cholesterol crystals in human gallbladder bile [PDF]
, 1989 Rapid aggregation of cholesterol-phospholipid vesicles in gallbladder bile seems to be the first event in the production of cholesterol crystals, a prerequisite for cholesterol gallstone formation.
Jüngst, Dieter +1 more
core +1 more source