Results 41 to 50 of about 808,838 (234)

Optimization of a CRISPR/Cas9-mediated Knock-in Strategy at the Porcine Rosa26 Locus in Porcine Foetal Fibroblasts

open access: yesScientific Reports, 2017
Genetically modified pigs have important roles in agriculture and biomedicine. However, genome-specific knock-in techniques in pigs are still in their infancy and optimal strategies have not been extensively investigated.
Zicong Xie   +11 more
doaj   +1 more source

Highly efficient RNA-guided base editing in rabbit

open access: yesNature Communications, 2018
Base editors can make targeted changes without inducing a double-stranded break. Here, the authors apply the BE3 and ABE7.10 systems to rabbit to create highly efficient targeted base substitutions and various mutation types, and show reduced frequency ...
Zhiquan Liu   +6 more
doaj   +1 more source

Oct4 regulates DNA methyltransferase 1 transcription by direct binding of the regulatory element

open access: yesCellular & Molecular Biology Letters, 2018
Background The transcription factor Oct4 plays a pivotal role in the pre-implantation development of the mouse embryo. DNA methyltransferase 1 (Dnmt1) maintains the changes in DNA methylation during mammalian early embryonic development.
Fengrui Wu   +6 more
doaj   +1 more source

The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting.

open access: yesHuman Reproduction, 2011
BACKGROUND Many variations in oocyte and embryo grading make inter-laboratory comparisons extremely difficult. This paper reports the proceedings of an international consensus meeting on oocyte and embryo morphology assessment.
B. Balaban   +19 more
semanticscholar   +1 more source

Detrimental effects of lipopolysaccharides on maturation of bovine oocytes [PDF]

open access: yesAsian-Australasian Journal of Animal Sciences, 2019
Objective Gram-negative bacteria lipopolysaccharide (LPS) has been reported to be associated with uterine impairment, embryonic resorption, ovarian dysfunction, and follicle retardation.
Shanjiang Zhao   +5 more
doaj   +1 more source

Single-Step Genomic Prediction of Superovulatory Response Traits in Japanese Black Donor Cows

open access: yesBiology, 2023
We assessed the performance of single-step genomic prediction of breeding values for superovulatory response traits in Japanese Black donor cows. A total of 25,332 records of the total number of embryos and oocytes (TNE) and the number of good embryos ...
Atsushi Zoda   +6 more
doaj   +1 more source

QUANTITATIVE STUDIES OF THE GROWTH OF MOUSE EMBRYO CELLS IN CULTURE AND THEIR DEVELOPMENT INTO ESTABLISHED LINES

open access: yesJournal of Cell Biology, 1963
Disaggregated mouse embryo cells, grown in monolayers, underwent a progressive decline in growth rate upon successive transfer, the rapidity of the decline depending, among other things, on the inoculation density.
G. Todaro, H. Green
semanticscholar   +1 more source

AMH regulates granulosa cell function via ESR2/ p38-MAPK signaling pathway in sheep

open access: yesCommunications Biology
Anti-Müllerian Hormone (AMH), secreted by granulosa cells (GCs) of preantral follicles, regulates early-stage follicle growth by inhibiting the activation of primordial follicles.
Hui Xu   +7 more
doaj   +1 more source

Filtered reproductive long non-coding RNAs by genome-wide analyses of goat ovary at different estrus periods

open access: yesBMC Genomics, 2018
Background The goat is an important farm animal. Reproduction is an important process of goat farming. The ovary is the most important reproductive organ for goats.
Yong Liu   +17 more
doaj   +1 more source

YBX1 mediates alternative splicing and maternal mRNA decay during pre-implantation development

open access: yesCell & Bioscience, 2022
Background In mammals, maternal gene products decay and zygotic genome activation (ZGA) during maternal to zygotic transition (MZT) is critical for the early embryogenesis.
Mingtian Deng   +6 more
doaj   +1 more source

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