Results 21 to 30 of about 740,021 (309)

Engineered domain-inlaid Nme2Cas9 adenine base editors with increased on-target DNA editing and targeting scope

open access: yesBMC Biology, 2023
Background Nme2ABE8e has been constructed and characterized as a compact, accurate adenine base editor with a less restrictive dinucleotide protospacer-adjacent motif (PAM: N4CC) but low editing efficiency at challenging loci in human cells.
Ding Zhao   +11 more
doaj   +1 more source

The Genetic Mutation of ANO5 in Rabbits Recapitulates Human Cardiomyopathy

open access: yesApplied Sciences, 2020
The limb girdle muscular dystrophy type 2L (LGMD2L) is caused by mutations of the ANO5 gene in humans which encodes a 913 amino-acid integral membrane protein.
Tingting Sui   +5 more
doaj   +1 more source

Cloning of embryos [PDF]

open access: yesBioscientifica Proceedings, 2020
Nuclear transfer for the study of differentiation in amphibians has been used since the 1950s, but not until recently have the same procedures been applied successfully to some mammals. Nuclear transfer, as developed for the amphibian, is successful in sheep, cattle, rabbit, and pig, but not mouse embryos.
R S, Prather, N L, First
openaire   +2 more sources

A Study on Genes of Bayanbulak Sheep [PDF]

open access: yesAsian-Australasian Journal of Animal Sciences, 2013
The average twin lambing rate of Bayanbulak sheep is 2% to 3%. However, a flock of sheep with a close genetic relationship and an average of 2 to 3 lambs per birth has been found recently.
Beiyao Zuo   +10 more
doaj   +1 more source

ASSESSMENT OF THE EFFICACY O SEPHADEX FILTRATION OF BUFFALO SPERMATOZOA FOR CRYOPRESERVATION [PDF]

open access: yesVeterinary Medical Journal - Giza, 2006
In the present study, two experiments were conducted. The first experiment aimed to study the influence of buffalo bull semen filtration throughout different grades of sephadex columns (G-25, G-50, G-75, G-100 and G-200) on semen quality.
MAHA ZIADA   +3 more
doaj   +1 more source

Maternal Magnolol Supplementation during Pregnancy and Lactation Promotes Antioxidant Capacity, Improves Gut Health, and Alters Gut Microbiota and Metabolites of Weanling Piglets

open access: yesMetabolites, 2023
Maternal nutrition exerts a profound effect on the postnatal performance of offspring, especially during the weaning period. The multifunctional bioactive component magnolol (MAG) has shown promise as a dietary supplement. This study aimed to explore the
Qiwen Fan   +8 more
doaj   +1 more source

Global DNA methylation profiles of buffalo (Bubalus bubalis) preimplantation embryos produced by handmade cloning and in vitro fertilization

open access: yesScientific Reports, 2022
Somatic cell nuclear transfer technique (SCNT) has proved to be an outstanding method of multiplication of elite animals but accompanied with low efficiency and live birth rate of cloned animals. Epigenetic alterations of DNA has been one of the culprits
Shivani Malpotra   +4 more
doaj   +1 more source

Limb proportions show developmental plasticity in response to embryo movement

open access: yesScientific Reports, 2017
Animals have evolved limb proportions adapted to different environments, but it is not yet clear to what extent these proportions are directly influenced by the environment during prenatal development.
A. Pollard   +6 more
semanticscholar   +1 more source

CRISPR/Cas9-mediated mutation of tyrosinase (Tyr) 3′ UTR induce graying in rabbit

open access: yesScientific Reports, 2017
The 3′ untranslated regions (UTRs), located at the end of mRNA molecules, are believed to play a role in RNA replication and/or protein translation. Mutations in the tyrosinase (Tyr) gene are known to cause recessive albinism in humans and other species.
Yuning Song   +10 more
doaj   +1 more source

Robust generation of transgenic mice by simple hypotonic solution mediated delivery of transgene in testicular germ cells

open access: yesMolecular Therapy: Methods & Clinical Development, 2016
Our ability to decipher gene sequences has increased enormously with the advent of modern sequencing tools, but the ability to divulge functions of new genes have not increased correspondingly.
Abul Usmani   +8 more
doaj   +1 more source

Home - About - Disclaimer - Privacy