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Association of duration of embryo culture with risk of large for gestational age delivery in cryopreserved embryo transfer cycles. [PDF]
Fertil SterilPier BD +3 more
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2006
Human embryonic stem cells (hESCs) are derived from preimplantation embryos. Approximately 60% of human embryos are blocked during in vitro development. Although statistics are inconclusive, experience demonstrates that hESCs are more effectively derived from high-quality embryos.
Amparo, Mercader +2 more
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Human embryonic stem cells (hESCs) are derived from preimplantation embryos. Approximately 60% of human embryos are blocked during in vitro development. Although statistics are inconclusive, experience demonstrates that hESCs are more effectively derived from high-quality embryos.
Amparo, Mercader +2 more
openaire +2 more sources
Culture Systems: Embryo Co-Culture
2012During the 1970s, domestic animal biotechnology, i.e., embryo transfer in farm animals, was confronted with the problem of embryonic developmental arrest observed in vitro, especially during the cycle in which maternal to zygotic transition (MZT) cycle takes place.
Yves J R, Ménézo +4 more
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2012
Although the rabbit is used extensively in developmental toxicity testing, relatively little is known about the fundamental developmental biology of this species let alone mechanisms underlying developmental toxicity. This paucity of information about the rabbit is partly due to the historic lack of whole embryo culture (WEC) methods for the rabbit ...
Valerie A, Marshall, Edward W, Carney
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Although the rabbit is used extensively in developmental toxicity testing, relatively little is known about the fundamental developmental biology of this species let alone mechanisms underlying developmental toxicity. This paucity of information about the rabbit is partly due to the historic lack of whole embryo culture (WEC) methods for the rabbit ...
Valerie A, Marshall, Edward W, Carney
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Culture Media and Embryo Culture
2021Before considering the basis of embryo culture medium, it is worthwhile reflecting on its function. A detailed overview of embryo metabolism is given in Chapter 4; however, in brief, the embryo must satisfy changing demands for energy by consumption of nutrients from the external milieu (Lewis & Sturmey, 2015).
Sunde, Arne, Sturmey, Roger G.
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Embryo Culture and Epigenetics
2012During preimplantation development, major epigenetic reprogramming occurs, erasing gametic modifications, and establishing embryonic epigenetic modifications. Given the plasticity of these modifications, they are susceptible to disruption by assisted reproductive technologies, including embryo culture. The current state of evidence is presented for the
Brenna A Market, Velker +2 more
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Mammalian Preimplantation Embryo Culture
2013Since the inception of modern embryo culture media over 50 years ago there have been significant developments in culture systems for the mammalian preimplantation embryo. Carbohydrate gradients have been shown to impact embryo physiology and viability, while amino acids have been determined to have specific temporal effects during the preimplantation ...
Gardner, D.K., Lane, M.
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Whole embryo culture of presomitic mouse embryos
Toxicology in Vitro, 1995Rat embryos explanted at the presomite stage and cultured through limb bud stages develop to well formed embryos and exhibit growth and differentiation which mimic those observed in vivo at corresponding stages. In contrast, the culture of presomite stage mouse embryos has proven to be much less successful.
G, Van Maele-Fabry +2 more
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2019
The embryotoxicity associated with exposure to exogenous compounds such as drugs and environmental chemicals can be assessed using the mouse whole embryo culture technique. This method has several advantages over traditional in vivo studies including the exclusion of any confounding maternal and placental effects, the selection of embryos that are at ...
Emily W Y, Tung, Louise M, Winn
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The embryotoxicity associated with exposure to exogenous compounds such as drugs and environmental chemicals can be assessed using the mouse whole embryo culture technique. This method has several advantages over traditional in vivo studies including the exclusion of any confounding maternal and placental effects, the selection of embryos that are at ...
Emily W Y, Tung, Louise M, Winn
openaire +2 more sources