Results 191 to 200 of about 822,085 (315)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

National Center for Emerging and Zoonotic Infectious Diseases accomplishments 2022

open access: yes
CDC\u2019s National Center for Emerging and Zoonotic Infectious Diseases (NCEZID) works to protect people in the United States and around the world by preventing, detecting, and responding to outbreaks and emergences of infectious diseases.In 2022 ...

core  

Strategic planning of prevention and surveillance for emerging diseases and invasive species. [PDF]

open access: yesProc Natl Acad Sci U S A
Wang J   +10 more
europepmc   +1 more source

Viral Emerging Diseases: Challenges in Developing Vaccination Strategies. [PDF]

open access: yesFront Immunol, 2020
Trovato M   +4 more
europepmc   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

open access: yesFEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe   +5 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

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