Results 51 to 60 of about 608,244 (260)
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
FEBS Open Bio, EarlyView.Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription
FEBS Open Bio, EarlyView.In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang +10 more
wiley +1 more source
Methodology of Pedagogical Research
Science & Philosophy, 2018 The pedagogical research constitutes a critical, dynamic, intentional, active process oriented towards the investigation of the educaional reality under all its aspects.
Cristina Ispas
doaj +1 more source
Empirical methods in the analysis of collusion [PDF]
Empirica, 2011 Regression methods are commonly used in competition lawsuits for, e.g., determining overcharges in pricefixing cases. Technical evaluations of these methods' pros and cons are not necessarily intuitive. Appraisals that are based on case studies are descriptive but need not be universally valid.
openaire +4 more sources
Agile Methods in Ethiopia: An Empirical Study [PDF]
, 2017 This paper provides empirical evidence of agile method adoption in smaller companies in Ethiopia. Agile methods are emerging as best practice for software development in the global north. So, is there evidence that agile methods are being used in Ethiopia?
Zelalem Regassa +2 more
openaire +1 more source
FEBS Open Bio, EarlyView.This pilot study investigates the potential of Generative AI to provide formative feedback to students. ChatGPT was prompted to provide feedback on Year 1 Bioscience essays, which were evaluated against established good feedback practices. GenAI‐authored feedback had useful elements, but was limited in scope. GenAI may have potential to provide instant,
Annabel Court +3 more
wiley +1 more source
Remote SensingBathymetric data are crucial for benthic monitoring in coastal areas but are traditionally obtained through costly and geographically limited acoustic methods.
Fickrie Muhammad +2 more
doaj +1 more source
FEBS Open Bio, EarlyView.The PAM pathway and its impact on cellular metabolism. Color‐coded key symbols represent Rapamycin (R, orange, targeting mTOR) and Dactolisib (D, green, targeting PI3K/mTOR), indicating their sites of action. Created with BioRenders.com. Glioblastoma resists therapy through interconnected survival programs.
Kai Zhao +23 more
wiley +1 more source
The Steepest Descent Method Using the Empirical Mode Gradient Decomposition
Proceedings of the International Conference on Applied Innovations in IT, 2020 The aim of the article is to study the possibility of improving gradient optimization methods. The leading approach to the chosen concept is based on the possibility of a featured description of the gradient that sets the direction of the search for a ...
Vasiliy Esaulov, Roman Sinetsky
doaj +1 more source