Results 141 to 150 of about 364,848 (300)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Virtual Fitting of Personalized Knitwear

open access: yes, 2019
The aim of this project was to build a pleasant customer experience of personalizing, virtual fitting and ordering made-to-measure knitwear, so as to improve their satisfaction on fit as well as reduce waste in the fashion industry.
Zhou, Wenjun (author)
core  

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Predicting Failure of Tongue Compression End Fittings

open access: yes
A common form of cantilever used to support the overhead line equipment is assembled from steel tubes with crimped in end fittings. Within the older UK stock, these end fittings have been seen to come loose.
Barrans, Simon   +2 more
core   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

open access: yesFEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada   +5 more
wiley   +1 more source

Study of mwcnts filled toughened epoxy for anchoring composite tensile armours inside the end fitting of flexible riser

open access: yes, 2017
International audienceThe use of composite and nanocomposite materials is a growing trend in many industries such as aerospace, automotive, civil construction and energy.
Do, Anh-Tuan   +4 more
core  

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

open access: yesFEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth   +6 more
wiley   +1 more source

Evaluation of Curve Fitting Techniques for Estimating End of Primary Consolidation Settlement: Provo Westside Connector Project, Utah [PDF]

open access: yes
15-8263This final report discusses the installation of magnet extensometer instrumentation associated with the Provo Westside Connector construction project in Utah County, Utah.
Pister, Christopher   +2 more
core  

Fitting curves of εp-end -σd relation of salinized silt under different confining pressures (RC= 94%, σ3=60 kPa).

open access: yes
Fitting curves of εp-end -σd relation of salinized silt under different confining pressures (RC= 94%, σ3=60 kPa).
Jing Yuan Kou (18115001)   +5 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

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