Results 101 to 110 of about 2,428 (138)

Sensitive detection of endo-1,4-β-glucanases and endo-1,4-β-xylanases in gels

Analytical Biochemistry, 1985
A simple, highly sensitive zymogram technique for detection of endo-1,4-beta-glucanases and endo-1,4-beta-xylanases in polyacrylamide gels after electrophoresis or isoelectric focusing was developed. The detection employs transparent agar replicas containing soluble covalently dyed polysaccharides, hydroxyethylcellulose dyed with Ostazin brilliant red ...
Peter Biely, Oskar Markovic
exaly   +3 more sources

Structural and functional properties of low molecular weight endo-1,4-β -xylanases

Journal of Biotechnology, 1997
There are currently four crystal structures of low molecular weight endo-1,4-beta-xylanases (E.C.3.2.1.8), i.e. family G/11 xylanases, available at the Brookhaven Data Bank: 2 xylanases from Trichoderma reesei (Törrönen et al., 1994; Törrönen and Rouvinen, 1995) and one from Bacillus circulans and another from Trichoderma harzianum (Campbell et al ...
Juha Rouvinen, A Törrönen
exaly   +3 more sources

Substrate-Binding Site of Endo-1,4-beta-Xylanase of the Yeast Cryptococcus albidus

European Journal of Biochemistry, 1981
The substrate-binding site of endo-1,4-beta-xylanase of the yeast Cryptococcus albidus was investigated using, 1,4-beta-xylooligosaccharides (1-3H)-labelled at the reducing end. Evaluation of the affinities of ten imaginary subsites by the method of Suganuma et al. [1978, J. Biochem.
P, Biely, Z, Krátký, M, Vrsanská
openaire   +2 more sources

Optimization of the Trichoderma reesei endo-1,4-beta-xylanase production by recombinant Pichia pastoris

Biochemical Engineering Journal, 2010
Abstract In this study, an optimization of xylanase expression by recombinant Pichia pastoris was carried out with a 2 3 factorial design. The influence of initial cell density, methanol and yeast nitrogen base concentration was evaluated, and the cell density was found to be the most important parameter.
Jun He, Daiwen Chen, Bing Yu, Keying Zhang   +3 more
openaire   +1 more source

Mechanisms of Substrate Digestion by Endo-1,4-beta-Xylanase of Cryptococcus albidus. Lysozyme-Type Pattern of Action

European Journal of Biochemistry, 1981
The action pattern and reaction mechanism of the endo-1,4-beta-xylanase of the yeast Cryptococcus albidus were investigated using reducing-end (1-3H)-labelled and uniformly 14C-labelled beta-1,4-xylooligosaccharides up to xylopentaose. The enzyme was found to catalyze degradation of oligosaccharides also by other pathways than a simple hydrolytic ...
P, Biely, M, Vrsanská, Z, Krátký
openaire   +2 more sources

Purification and characterization of two endo-1,4-beta-xylanases and a 3-xylosidase from phlebia radiata.

Acta microbiologica Polonica, 2002
Two different endo-1,4-beta-xylanases, designated XA-1 and XA-2, and one beta-xylosidase (XD-1) have been purified by column chromatography to apparent homogeneity from the extracellular culture fluid of Phlebia radiata grown on wheat bran. The molecular masses of XA- 1, XA-2 and XD-1 were 18.6, 15.8 kDa, and 27 kDa, respectively.
J, Rogalski, M, Oleszek, J, Tokarzewska-Zadora   +2 more
openaire   +2 more sources

Endo-1,4-beta-xylanase with traces of endo-1,4-beta-glucanase improves the performance of weanling pigs supplied diets reduced in 90 kcal metabolisable energy/kg

Archives of Animal Nutrition
This experiment was carried out to evaluate the effect of endo-1,4-beta-xylanase with traces of endo-1,4-beta-glucanase on the performance of 240 piglets (Landrace × Yorkshire × Duroc) with an initial body (BW) of 5.90 ± 0.6 kg. The mixed sex piglets were randomly assigned to 3 treatments with 10 replicates per treatment, and 8 heads per replicate pen.
Sang Hun Ha, Habeeb Tajudeen, Abdolreza Hosseindoust, Jun Young Mun, Priscilla Neves, Elick Kinara, Se Rin Park, Santosh Ingale, Anushka Lokhande, JinSoo Kim   +9 more
openaire   +2 more sources

The active site of an acidic endo-1,4-β-xylanase of Aspergillus niger

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1983
The substrate binding site of an acidic endo-1,4-beta-xylanase (1,4-beta-D-xylan xylanohydrolase, EC 3.2.1.8) of Aspergillus niger was investigated using 1,4-beta-xylooligosaccharides (1-3H)-labelled at the reducing end. Bond cleavage frequencies and V/Km parameters of the oligosaccharides were determined under conditions of unimolecular hydrolysis and,
P, Biely, M, Vrsanská, I V, Gorbacheva
openaire   +2 more sources

Functional identification of the cDNA coding for a wheat endo-1,4-beta-D-xylanase inhibitor.

FEBS letters, 2002
Using expressed sequence tag data, we obtained a full-length cDNA encoding a wheat protein inhibitor of xylanases (XIP-I). The 822 bp open reading frame encoded a protein of 274 amino acids with a molecular mass of 30.2 kDa, in excellent agreement with the native protein.
Giles O, Elliott, Richard K, Hughes, Nathalie, Juge, Paul A, Kroon, Gary, Williamson   +4 more
openaire   +1 more source

An endo-1,4-β-xylanase-encoding gene from Agaricus bisporus is regulated by compost-specific factors

Journal of Molecular Biology, 1998
Compost is the preferred substrate for growth of the edible fungus Agaricus bisporus. Utilization of compost requires the production of enzymes involved in degradation of lignocellulolytic components. For molecular characterization of these processes we are isolating the encoding genes. By applying heterologous screening techniques, we have cloned such
de Groot, P.W.J., Basten, E.J.W., Sonnenberg, A.S.M., van Griensven, L.J.L.D., Visser, J., Schaap, P.J.   +5 more
openaire   +3 more sources