Results 291 to 300 of about 271,229 (377)

Evaluating the Benefits and Limits of Multiple Displacement Amplification With Whole‐Genome Oxford Nanopore Sequencing

Molecular Ecology Resources, Volume 25, Issue 6, August 2025.
ABSTRACT Multiple displacement amplification (MDA) outperforms conventional PCR in long fragment and whole‐genome amplification, making it attractive to couple MDA with long‐read sequencing of samples with limited quantities of DNA to obtain improved genome assemblies.
Fiifi Agyabeng‐Dadzie, Megan S. Beaudry, Alex Deyanov, Haley Slanis, Minh Q. Duong, Randi Turner, Asis Khan, Cesar A. Arias, Jessica C. Kissinger, Travis C. Glenn, Rodrigo de Paula Baptista   +10 more
wiley   +1 more source

Efficacy of baloxavir marboxil as a prophylactic against influenza among in-patients and analysis of influenza virus mutations in their isolates at a Japanese hospital during the 2023-2024 flu season. [PDF]

J Pharm Health Care Sci
Fujii A, Oishi T, Kondo E, Morihara Z, Ikeda Y, Sumida H, Ninomiya Y, Uesugi S, Okazaki K, Yoshioka D, Kobayashi M, Wada H.   +11 more
europepmc   +1 more source

Unstable anthocyanin pigmentation in Streptocarpus sect. Saintpaulia (African violet) is due to transcriptional selectivity of a single MYB gene

New Phytologist, Volume 247, Issue 3, Page 1426-1444, August 2025.
Summary Saintpaulia (African violet) pigmentation is notoriously unstable and sometimes forms white stripes, particularly following passage through tissue culture. White‐striped petals were thought to be due to periclinal chimeras, but we confirmed that white stripes result from epigenetic regulation rather than periclinal chimeras based on the flower ...
Daichi Kurata, Tomohisa Tsuzaki, Fumi Tatsuzawa, Kenta Shirasawa, Hideki Hirakawa, Munetaka Hosokawa   +5 more
wiley   +1 more source

Cas9 endonuclease: a molecular tool for <i>in vitro</i> cloning and CRISPR edit detection. [PDF]

Front Genome Ed
Ma X, Kthiri D, Gill MS, Pozniak CJ, Kagale S.   +4 more
europepmc   +1 more source

Optimisation of Heated Electrospray Ionisation Parameters to Minimise In‐Source Generated Impurities in the Analysis of Oligonucleotide Therapeutics

Rapid Communications in Mass Spectrometry, Volume 39, Issue 13, 15 July 2025.
ABSTRACT Rationale Oligonucleotides have emerged as an important new class of therapeutic. Due to their structural complexity, this presents significant challenges for the development of analytical methods to characterise and determine their impurity profile.
Mollie A. Glenister, Ulrik Mistarz, Ken Cook, James L. Stephenson, Mark J. Dickman   +4 more
wiley   +1 more source

A method for creating in-frame insertions of fluorescent proteins in non-model gram-negative bacteria. [PDF]

AMB Express
Chandramowli D, Devreese B.
europepmc   +1 more source

Enhanced discovery of bacterial laccase‐like multicopper oxidase through computer simulation and metagenomic analysis of industrial wastewater

FEBS Open Bio, Volume 15, Issue 7, Page 1090-1102, July 2025.
We obtained potential bacterial laccase‐like multicopper oxidase (LMCO) sequences through metagenomic sequencing. All sequences exhibited significant differences from known LMCOs in databases. To select the most promising candidates, we performed structure prediction and molecular docking using alphafold2, metal3d and rosetta.
Ting Cui, Ariel Kushmaro, Hana Barak, Anja Poehlein, Rolf Daniel, Hans‐Jürgen Mägert   +5 more
wiley   +1 more source

Influenza A(H1N1)pdm09 Virus with Reduced Susceptibility to Baloxavir, Japan, 2024. [PDF]

Emerg Infect Dis
Takashita E, Morita H, Nagata S, Fujisaki S, Miura H, Ikeda T, Komabayashi K, Sasaki M, Matoba Y, Takahashi T, Ogawa N, Mizuta K, Ito S, Kishida N, Nakamura K, Shirakura M, Watanabe S, Hasegawa H.   +17 more
europepmc   +1 more source

Protonated Glutamate and Aspartate Side Chains Can Recognize Phosphodiester Groups via Strong and Short Hydrogen Bonds in Biomacromolecular Complexes

Angewandte Chemie International Edition, Volume 64, Issue 27, July 1, 2025.
The highly negatively charged phosphodiester groups of nucleic acids and cyclic (di‐)nucleotides often interact with amino acids containing polar or positively charged sidechains. Due to charge repulsion with their negatively charged carboxylate groups in their side chains, glutamate and aspartate are considered to be detrimental to such interactions ...
Konstantin Neißner, Elke Duchardt‐Ferner, Christoph Wiedemann, Julian Kraus, Ute A. Hellmich, Jens Wöhnert   +5 more
wiley   +1 more source

Mutation in soybean Lox-2 PLAT/LH2 domain through CRISPR/Cas9 reduces seed lipoxygenase activity: responsible for undesirable flavour. [PDF]

Transgenic Res
Patel E, Das P, Hazra S, Sharma M, Chhabra G, Gill BS, Sharma S, Kaur A, Singla D, Sandhu JS.   +9 more
europepmc   +1 more source