Advanced Science, Volume 13, Issue 4, 19 January 2026.The pathogenic role of PHEX isn't fully determined, and there is no radical cure for X‐linked hypophosphatemic rickets (XLHR). This study makes the first attempt to perform gene therapy using a minicircle DNA (MC‐DNA) vector expressing a fragment of FGF23 (amino acids 180‐251) in Phex‐T1349C mice and suggests MC‐DNA as a promisingly safe and effective ...
Huixiao Wu +20 more
wiley +1 more source
Advanced Science, Volume 13, Issue 1, 5 January 2026.Two NO‐containing iron units, biologically relevant as NO storage/release centers, connected by sulfur, display characteristics arising from multiple quantum levels in their extensively delocalized arrangement. Analysis in three charge levels finds the electron distribution almost substantially spread over all atoms of its composition: 2S, 2Fe, and 3NO.
Sarnali Sanfui +11 more
wiley +1 more source
Molecular Characterization of a Restriction Endonuclease PsaI from <i>Pseudomonas anguilliseptica</i> KM9 and Sequence Analysis of the PsaI R-M System. [PDF]
Int J Mol SciFurmanek-Blaszk B, Mruk I, Sektas M.
europepmc +1 more source
Advanced Science, Volume 13, Issue 1, 5 January 2026.This study reveals that the deubiquitinase USP11 stabilizes PGAM5, triggering neurotoxic astrocyte transformation after intracerebral hemorrhage. PGAM5 promotes mPTP opening and Drp1 dephosphorylation, synergistically amplifying mtDNA leakage into the cytosol and leading to cGAS–STING hyperactivation.
Jiaqing He +10 more
wiley +1 more source
Division of labor: OEX1 and POL1A/POL1B are partial derivatives of prokaryotic DNA polymerase required for mitochondrial genome maintenance in plants. [PDF]
Plant CellKolli R.
europepmc +1 more source
RNAi Regulator C3PO Promotes Arbovirus Infection in Insect Vectors
Advanced Science, Volume 13, Issue 4, 19 January 2026.RNA interference (RNAi) is vital for eukaryotes to defend against virus infection. The C3PO complex takes part in RNAi but its role in regulating viral infection remains unclear. This work finds that insect C3PO facilitates arbovirus replication by degrading precursors of miRNAs, especially miR‐971‐3p.
Yan Xiao +7 more
wiley +1 more source
RAPID-DASH: fast and efficient assembly of guide RNA arrays for multiplexed CRISPR-Cas9 applications. [PDF]
Synth Biol (Oxf)Salaudeen AL, Mateyko N, de Boer CG.
europepmc +1 more source
The Double Face of Base Excision Repair: Preventing and Triggering Double‐Strand Breaks
BioEssays, Volume 48, Issue 1, January 2026.Base excision repair is conserved from bacteria to man, robustly eliminating oxidized bases in DNA and reinserting the appropriate nucleotide to restore an intact double helix. Recent results describe conditions and mechanisms that misregulate long‐patch BER to generate double‐strand breaks, triggering chromosome fragmentation and cell death, altering ...
Susan M. Gasser
wiley +1 more source
A Diagnostic Approach to Separate Acute Human Bocavirus 1 Respiratory Tract Infection From Long-lasting Virus Shedding. [PDF]
J Infect DisRayamajhi Thapa R +4 more
europepmc +1 more source
Engineering of High‐Yield Recombinant Adeno‐Associated Virus Producer Plasmids
Biotechnology Journal, Volume 21, Issue 1, January 2026.Graphical Abstract and Lay Summary Recombinant adeno‐associated viruses (AAV) are important in gene therapy but their production may be impaired by non‐optimized gene design. In this study, the authors demonstrate that production was best when the AAV replicase gene was in a wild‐type configuration while the complexity of other genetic elements could ...
Marco T. Radukic +10 more
wiley +1 more source