Results 341 to 350 of about 272,760 (374)
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Endonucleases and apoptosis in animals
Biochemistry (Moscow), 2012Endonucleases are the main instruments of obligatory DNA degradation in apoptosis. Many endonucleases have marked processive action; initially they split DNA in chromatin into very large domains, and then they perform in it internucleosomal fragmentation of DNA followed by its hydrolysis to small fragments (oligonucleotides).
N. I. Aleksandrushkina +1 more
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Characterization of mRNA Endonucleases
Methods, 1999Endonucleases are key effectors of mRNA degradation, particularly for mRNAs whose turnover rates are regulated by extracellular stimuli. The rapid clearance of mRNA degradation products in vivo and the need to selectively identify mRNA endonucleases in the presence of many other cellular ribonucleases make the study of these enzymes particularly ...
Daniel R. Schoenberg +1 more
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Digestion of DNA with Restriction Endonucleases [PDF]
Current Protocols in Neuroscience, 1992 AbstractRestriction endonucleases recognize short DNA sequences and cleave double‐stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology.
K D, Bloch, B, Grossmann
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Journal of Medicinal Chemistry, 2017
Influenza virus (IFV) causes periodic global influenza pandemics, resulting in substantial socioeconomic loss and burden on medical facilities. Yearly variation in the effectiveness of vaccines, slow responsiveness to vaccination in cases of pandemic IFV,
Han Ju +6 more
semanticscholar +1 more source
Influenza virus (IFV) causes periodic global influenza pandemics, resulting in substantial socioeconomic loss and burden on medical facilities. Yearly variation in the effectiveness of vaccines, slow responsiveness to vaccination in cases of pandemic IFV,
Han Ju +6 more
semanticscholar +1 more source
Restriction endonucleases in Azospirillum
Gene, 1985Azospirillum brasilense, A. amazonense, and A. lipoferum strains were screened for restriction endonucleases using phage lambda DNA. The extract of A. brasilense 29711 cleaved lambda DNA into specific fragments. It was concluded that this strain possesses a class II restriction endonuclease which was named AbrI.
Gerhard Schwabe +2 more
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Homing in on an endosymbiotic endonuclease
Current Biology, 1992There was a time when life as a molecular biologist was relatively simple: genes were genes easily recognizable by a beginning, an end and a coding sequence in between. The arrival of introns complicated things for a while: one had to get used to the idea that some genes were in pieces, perhaps always had been.
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Journal of Immunology, 1984
Dexamethasone and corticosterone kill mouse thymocytes, as measured by eosin uptake, after several hours of in vitro incubation. This killing requires RNA and protein synthesis, because it is inhibited by cycloheximide, emetine, or actinomycin D.
J. Cohen, R. Duke
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Dexamethasone and corticosterone kill mouse thymocytes, as measured by eosin uptake, after several hours of in vitro incubation. This killing requires RNA and protein synthesis, because it is inhibited by cycloheximide, emetine, or actinomycin D.
J. Cohen, R. Duke
semanticscholar +1 more source
Nucleosomes Inhibit Cas9 Endonuclease Activity in Vitro.
Biochemistry, 2015During Cas9 genome editing in eukaryotic cells, the bacterial Cas9 enzyme cleaves DNA targets within chromatin. To understand how chromatin affects Cas9 targeting, we characterized Cas9 activity on nucleosome substrates in vitro.
J. M. Hinz +2 more
semanticscholar +1 more source
Endonuclease from Proteus mirabilis
Applied Biochemistry and Microbiology, 2001Two isoforms of nuclease displaying DNase and RNase activities were found in the culture liquid and periplasm of Proteus mirabilis. The enzyme was isolated from the periplasm and then purified to a functionally homogeneous state. The nuclease was equally potent in cleaving denatured and native DNAs by the endonuclease mechanism and was designated Pm ...
Salikhova Z. +3 more
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Characterization of the Escherichia coli x-ray endonuclease, endonuclease III
Biochemistry, 1983The X-ray endonuclease endonuclease III of Escherichia coli has been purified to apparent homogeneity by using the criterion of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The most purified fraction shows endonucleolytic activity against apurinic and apyrimidinic (AP) sites and a dose-dependent response to DNA that has been X irradiated,
Susan S. Wallace, Harold L. Katcher
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