Results 351 to 360 of about 272,760 (374)

Endonuclease of T4 ghosts

Virology, 1977
Abstract An endonuclease was found to associate with purified T4 particles. Its activity was low with intact phages but became distinct when phage particles were disrupted by osmotic shock, and more distinct when they were disassembled by guanidine.
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A homing endonuclease with a switch: Characterization of a twintron encoded homing endonuclease

Fungal Genetics and Biology, 2014
The small ribosomal subunit gene residing in the mitochondrial DNA of the thermophilic fungus Chaetomium thermophilum var. thermophilum La Touche DSM 1495 is interrupted by a twintron at position mS1247. The mS1247 twintron represents the first mixed twintron found in fungal mtDNA, composed of an external group I intron encoding a LAGLIDADG open ...
Georg Hausner, Tuhin Kumar Guha
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[2] Directory of restriction endonucleases

1979
Publisher Summary This chapter serves as a directory to the restriction endonucleases that have not been characterized. All endonucleases which cleave DNA at a specific sequence have been considered to be restriction enzymes, although in most cases there is no direct genetic evidence for the presence of a host-controlled restriction-modification ...
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Ultraviolet Damage Endonuclease (Uve1p):  A Structure and Strand-Specific DNA Endonuclease

Biochemistry, 2000
Schizosaccharomyces pombe ultraviolet damage endonuclease (UVDE or Uve1p) performs the initial step in an alternative excision repair pathway for UV-induced DNA damage. This DNA repair pathway was originally thought to be specific for UV damage. However, the broad substrate specificity of Uve1p suggests a more general role for this enzyme.
Paul W. Doetsch, Balveen Kaur
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An Endonuclease Tango

Science, 2008
BIOCHEMISTRY The degradation of mRNA transcripts is an important cellular mechanism contributing to the regulation of gene expression. In Escherichia coli , the first step of this process involves internal cleavage of mRNA into fragments by the endoribonuclease RNase E.
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An endonuclease at last

Nature, 1998
One of the main clues that apoptosis is a ‘programmed’ cell death is the production of ‘chromatin ladders’ — digestion of DNA into regularly sized fragments. The nuclease responsible for this cleavage has now been identified. Found as an inactive form within the cytoplasm, this nuclease is activated by caspase-3 digestion, earning it the name caspase ...
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The Type IIB restriction endonucleases

Biochemical Society Transactions, 2010
The endonucleases from the Type IIB restriction–modification systems differ from all other restriction enzymes. The Type IIB enzymes cleave both DNA strands at specified locations distant from their recognition sequences, like Type IIS nucleases, but they are unique in that they do so on both sides of the site, to liberate the site from the remainder ...
Marshall, JJT, Halford, SE
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The hunt for the 3^′endonuclease [PDF]

WIREs RNA, 2010
AbstractPre‐mRNAs are typically processed at the 3′end by cleavage/polyadenylation. This is a two‐step processing reaction initiated by endonucleolytic cleavage of pre‐mRNAs downstream of the AAUAAA sequence or its variant, followed by extension of the newly generated 3′end with a poly(A) tail.
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The Use of Restriction Endonucleases

2003
The discovery of the mode of action of the class of bacterial enzymes known as restriction endonucleases provided the major breakthrough in opening up the field of genetic engineering. In vivo, these enzymes are involved in recognizing and cutting up foreign DNA entering the cell; their most likely role is thus protecting the bacteria against phage ...
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Restriction Endonuclease Digestion of DNA

2003
The ability to cleave DNA at specific sites is one of the cornerstones of today's methods of DNA manipulation. Restriction endonucleases are bacterial enzymes that cleave duplex DNA at specific target sequences with the production of defined fragments. These enzymes can be purchased from the many manufacturers of biotechnology products.
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