Results 51 to 60 of about 299,455 (330)

Molecular mechanisms of Dicer: endonuclease and enzymatic activity

Biochemical Journal, 2017
The enzyme Dicer is best known for its role as a riboendonuclease in the small RNA pathway. In this canonical role, Dicer is a critical regulator of the biogenesis of microRNA and small interfering RNA, as well as a growing number of additional small ...
Min‐sun Song, J. Rossi
semanticscholar   +1 more source

In vitro Inactivation of Latent HSV by Targeted Mutagenesis Using an HSV-specific Homing Endonuclease

Molecular Therapy: Nucleic Acids, 2014
Following acute infection, herpes simplex virus (HSV) establishes latency in sensory neurons, from which it can reactivate and cause recurrent disease.
Martine Aubert, Nicole M Boyle, Daniel Stone, Laurence Stensland, Meei-Li Huang, Amalia S Magaret, Roman Galetto, David J Rawlings, Andrew M Scharenberg, Keith R Jerome   +9 more
doaj   +1 more source

The teneurin C-terminal domain possesses nuclease activity and is apoptogenic. [PDF]

, 2018
Teneurins are type 2 transmembrane proteins expressed by developing neurons during periods of synaptogenesis and apoptosis. Neurons expressing teneurin-1 synapse with other teneurin-1-expressing neurons, and neurons expressing teneurin-2 synapse with ...
Chiquet-Ehrismann, Ruth, Ferralli, Jacqueline, Tucker, Richard P   +2 more
core   +7 more sources

BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]

, 2016
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien, Callewaert, Bert, Coucke, Paul, De Paepe, Anne, De Rocker, Nina, Menten, Björn, Steyaert, Wouter, Willaert, Andy   +7 more
core   +2 more sources

A Hyperthermophilic Argonaute From Ferroglobus placidus With Specificity on Guide Binding Pattern

Frontiers in Microbiology, 2021
Argonaute proteins (Agos) from thermophilic archaea are involved in several important processes, such as host defense and DNA replication. The catalytic mechanism of Ago from different microbes with great diversity and genome editing potential is ...
Xiang Guo, Yingying Sun, Liuqing Chen, Fei Huang, Qian Liu, Yan Feng   +5 more
doaj   +1 more source

The subnuclear localization of tRNA ligase in yeast [PDF]

, 1987
Yeast tRNA ligase is an enzyme required for tRNA splicing. A study by indirect immune fluorescence shows that this enzyme is localized in the cell nucleus. At higher resolution, studies using indirect immune electron microscopy show this nuclear location
Abelson, John, Clark, Michael W.
core   +1 more source

Liquid biopsy epigenetics: establishing a molecular profile based on cell‐free DNA

Molecular Oncology, EarlyView.
Cell‐free DNA (cfDNA) fragments in plasma from cancer patients carry epigenetic signatures reflecting their cells of origin. These epigenetic features include DNA methylation, nucleosome modifications, and variations in fragmentation. This review describes the biological properties of each feature and explores optimal strategies for harnessing cfDNA ...
Christoffer Trier Maansson, Anders Lade Nielsen, Boe Sandahl Sorensen   +2 more
wiley   +1 more source

Class IIa HDACs forced degradation allows resensitization of oxaliplatin‐resistant FBXW7‐mutated colorectal cancer

Molecular Oncology, EarlyView.
HDAC4 is degraded by the E3 ligase FBXW7. In colorectal cancer, FBXW7 mutations prevent HDAC4 degradation, leading to oxaliplatin resistance. Forced degradation of HDAC4 using a PROTAC compound restores drug sensitivity by resetting the super‐enhancer landscape, reprogramming the epigenetic state of FBXW7‐mutated cells to resemble oxaliplatin ...
Vanessa Tolotto, Nicolò Gualandi, Ylenia Cortolezzis, Raffaella Picco, Monica Colitti, Francesca D'Este, Mariachiara Gani, Wayne W. Hancock, Giovanni Terrosu, Cristina Degrassi, Francesca Agostini, Claudio Brancolini, Luigi E. Xodo, Eros Di Giorgio   +13 more
wiley   +1 more source

Plant-RRBS, a bisulfite and next-generation sequencing-based methylome profiling method enriching for coverage of cytosine positions [PDF]

, 2017
Background: Cytosine methylation in plant genomes is important for the regulation of gene transcription and transposon activity. Genome-wide methylomes are studied upon mutation of the DNA methyltransferases, adaptation to environmental stresses or ...
A Akalin, A Meissner, A Verkest, AJ Bewick, AR Elhamamsy, AR Quinlan, B Langmead, Bram Slabbinck, C Becker, CA Ibarra, Cindy Martens, D Meng, D Pignatta, EJ Finnegan, F Johannes, Frederik Coppens, G Gremme, H Parkinson, H Saze, H Schöb, H Stroud, H Zhang, H-Q Wang, HJ Xie, International Rice Genome Sequencing Project, J Du, J Yu, JI Gent, K Manning, K Okonechnikov, M Block De, M Block De, M Choi, M Hauben, M Schmidt, Magdalena Woloszynska, Marc De Block, Martin Schmidt, MD Schultz, MG Murray, Michiel Van Bel, Mieke Van Lijsebettens, P Cubas, PF Gugger, PS Schnable, R Lister, RJ Schmitz, SE Jacobsen, SJ Cokus, T-F Hsieh, The Arabidopsis Genome Initiative, TJ Treangen, TP Gurp van, W Guo, X Cao, X Chen, X Li, X Wang, X Zhang, ZD Smith, ZL Liu   +60 more
core   +3 more sources

Peroxidasin enables melanoma immune escape by inhibiting natural killer cell cytotoxicity

Molecular Oncology, EarlyView.
Peroxidasin (PXDN) is secreted by melanoma cells and binds the NK cell receptor NKG2D, thereby suppressing NK cell activation and cytotoxicity. PXDN depletion restores NKG2D signaling and enables effective NK cell–mediated melanoma killing. These findings identify PXDN as a previously unrecognized immune evasion factor and a potential target to improve
Hsu‐Min Sung, David Bickel, Lena C. M. Krause, Daria Ezeriņa, Christian Ickes, Julian Wojtachnia, Christine S. Gibhardt, Magdalena Shumanska, Khadija Wahni, Andrea Paluschkiwitz, Julia Malo Pueyo, Ekaterina Baranova, Wim Vranken, Hedwig Stanisz, Ioana Stejerean‐Todoran, Michael P. Schön, Joris Messens, Ivan Bogeski   +17 more
wiley   +1 more source