Results 81 to 90 of about 271,229 (377)

CircTP53/USP10/p53 signaling Axis as a Novel Regulator of Progression and Prognosis of Head and Neck Squamous Cell Carcinoma

Advanced Science, EarlyView.
The study identifies a novel circular RNA derived from the TP53 gene (circTP53), which is upregulated in HNSCC and correlates with poor patient prognosis. It demonstrates that circTP53 promotes HNSCC progression by interacting with USP10, stabilizing both proteins, enhancing deubiquitination of p53, and thereby influencing tumor growth, with its ...
Yin Wang, Fen Chang, Zinan Li, Chengcheng Duan, Xiangkai Sun, Siyu Wang, Dongmin Wei, Wenming Li, Ye Qian, Shengda Cao, Juan Zhao, Dapeng Lei   +11 more
wiley   +1 more source

Single‐Molecule Protein Profiling Using Nanopores and Dimeric Aptamer‐Modified DNA Carriers

Angewandte Chemie, EarlyView.
We developed a nanopore‐based biosensor for single‐molecule protein profiling using aptamer‐modified DNA carrier dimerization strategy, enabling selective and ultrasensitive detection of proteins and protein complexes. This approach promises advances in early‐stage clinical diagnostics and precision medicine.
Xiaoyi Wang, Yaxian Liu, Ren Ren, Joshua B. Edel, Aleksandar P. Ivanov   +4 more
wiley   +2 more sources

Possible existence of a system similar to bacterial restriction–modification in plants

AIMS Molecular Science, 2020
The bacterial restriction–modification (R-M) system has evolved as a defense mechanism against infectious phages and other types of integration of foreign DNA fragments into the host chromosome through recombination.
Larisa I. Fedoreyeva, Boris F. Vanyushin   +1 more
doaj   +1 more source

Targeting human apurinic/apyrimidinic endonuclease 1 (APE1) in phosphatase and tensin homolog (PTEN) deficient melanoma cells for personalized therapy [PDF]

, 2014
Phosphatase and tensin homolog (PTEN) loss is associated with genomic instability. APE1 is a key player in DNA base excision repair (BER) and an emerging drug target in cancer.
Allinson, Ashworth, Ashworth, Bertram, Bosenberg, Botti, Bristow, Burma, Campisi, Curtin, Eng, Eng, Foiani, Gerson, He, Helleday, Hickson, Kelley, Larue, Li, Madhusudan, Madhusudan, Madhusudan, Madhusudan, Maloney, Maloney, Meyskens, Newton-Bishop, Pandolfi, Pandolfi, Parsons, Polyak, Pu, Reis-Filho, Reis-Filho, Ribas, Rimm, Robertson, Rocha, Simeonov, Simeonov, Smalley, Stambolic, Stambolic, Teo, Wadhwa, Wilson, Wilson, Yin, Zhang   +49 more
core   +2 more sources

Biosecurity Primitive: Polymerase X‐based Genetic Physical Unclonable Functions

Advanced Science, EarlyView.
What if every cell line can carry an unforgeable genomic fingerprint—one that proves its origin, ensures its authenticity, and thwarts unauthorized duplication? Here, CRISPR‐induced DNA lesions paired with TdT‐mediated repair produce robust, unique, and unclonable genetic signatures—advancing the concept of molecular Physical Unclonable Functions (PUF)
Zikun Zhou, Taek Kang, Jie Chen, Yesh Doctor, Jocelyn G Camposagrado, Yiorgos Makris, Alexander Pertsemlidis, Leonidas Bleris   +7 more
wiley   +1 more source

Decoy DNA Protects Molecular Tension Probes from DNase Degradation

Angewandte Chemie, EarlyView.
DNA‐based probes are rapidly degraded by DNases in live‐cell environments, limiting their use. We introduce a decoy DNA strategy, where an excess of unmodified double‐stranded DNA acts as a competitive substrate for DNases, protecting functional DNA probes and significantly extending their stability for long‐term live‐cell studies.
Hongyuan Zhang, Seong Ho Kim, Isaac T. S. Li   +2 more
wiley   +2 more sources

DNA polymerases required for repair of UV-induced damage in Saccharomyces cerevisiae [PDF]

, 1995
The ability of yeast DNA polymerase mutant strains to carry out repair synthesis after UV irradiation was studied by analysis of postirradiation molecular weight changes in cellular DNA.
Budd, Martin E., Campbell, Judith L.
core   +2 more sources

Endonucleases and simian virus 40 virions: origin of a virion-associated endonuclease [PDF]

Journal of Virology, 1976
The origin and role of the endonuclease activity associated with purified virions of simian virus 40, previously described by this and other laboratories, have been further investigated. We found that the enzymatic activity from virions of temperature-sensitive (ts) mutants is not more heat labile than that from wild-type virions.
H L Ozer, D C Dooley, M T Ryzlak
openaire   +3 more sources

Enhancing Specificity, Precision, Accessibility, Flexibility, and Safety to Overcome Traditional CRISPR/Cas Editing Challenges and Shape Future Innovations

Advanced Science, EarlyView.
CRISPR/Cas9, while transformative, faces challenges in specificity, precision, delivery, accessibility, flexibility, and safety. This review addresses these limitations by highlighting strategies to reduce off‐target effects, exploring HDR‐based and alternative editing approaches, and evaluating advanced delivery mechanisms.
Muna Alariqi, Mohamed Ramadan, Lu Yu, Fengjiao Hui, Amjad Hussain, Xiaofeng Zhou, Yu Yu, Xianlong Zhang, Shuangxia Jin   +8 more
wiley   +1 more source

Is a Malleable Active Site Loop the Key to High Substrate Promiscuity? Hybrid, Biocatalytic Route to Structurally Diverse Taxoid Side Chains with Remarkable Dual Stereocontrol

Angewandte Chemie, EarlyView.
A highly stereoselective, dynamic reductive kinetic resolution (DYRKR) entry into myriad Taxotere‐like side chains with Clostridium acetobutylicum alcohol dehydrogenase (CaADH) enzyme is reported. Follow‐on cross couplings expand the structural diversity of the library (36 total examples).
Gaurav P. Kudalkar, Florian Leidner, Nivesh Kumar, Jared L. Hass, Peter Madzelan, Douglas R. Powell, Victor W. Day, Pierre Le Magueres, Joseph D. Ferrara, Lee M. Daniels, Akihito Yamano, Sho Ito, Wei Niu, Helmut Grubmüller, Mark A. Wilson, David B. Berkowitz   +15 more
wiley   +2 more sources