Results 1 to 10 of about 24,423 (298)

Inhibition of anti-viral stress granule formation by coronavirus endoribonuclease nsp15 ensures efficient virus replication

open access: yesPLoS Pathogens, 2021
Cytoplasmic stress granules (SGs) are generally triggered by stress-induced translation arrest for storing mRNAs. Recently, it has been shown that SGs exert anti-viral functions due to their involvement in protein synthesis shut off and recruitment of ...
Huan Wang, Ying Liao, Yingjie Sun
exaly   +3 more sources

Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics

open access: yesNature Communications, 2021
Nsp15 is a uridine specific endoribonuclease present in all coronaviruses. Here, the authors determine the cryo-EM structures of SARS-CoV-2 Nsp15 in the apo and UTP-bound states, which together with biochemical experiments, mass spectrometry and ...
Cassandra K Hayne   +2 more
exaly   +3 more sources

An endoribonuclease-based feedforward controller for decoupling resource-limited genetic modules in mammalian cells

open access: yesNature Communications, 2020
Accurately predicting the behaviour of a genetic circuit remains difficult due to the lack of modularity. Here the authors quantify the effects of resource loading in mammalian systems and develop an endoribonuclease-based feedfoward controller to adapt ...
Ross D Jones   +2 more
exaly   +3 more sources

Tipiracil binds to uridine site and inhibits Nsp15 endoribonuclease NendoU from SARS-CoV-2

open access: yesCommunications Biology, 2021
Youngchang Kim, Jacek Wower, and colleagues explore the sequence specificity, metal ion dependence and catalytic mechanism of the Nsp15 endoribonuclease NendoU from SARS-CoV-2.
Jacek Wower   +2 more
exaly   +3 more sources

Coronavirus endoribonuclease targets viral polyuridine sequences to evade activating host sensors

open access: yesProceedings of the National Academy of Sciences of the United States of America, 2020
Significance Cells carry sensors that are primed to detect invading viruses. To avoid being recognized, coronaviruses express factors that interfere with host immune sensing pathways.
Matthew Hackbart   +2 more
exaly   +2 more sources

Endoribonuclease-mediated control of hns mRNA stability constitutes a key regulatory pathway for Salmonella Typhimurium pathogenicity island 1 expression.

open access: yesPLoS Pathogens, 2021
Bacteria utilize endoribonuclease-mediated RNA processing and decay to rapidly adapt to environmental changes. Here, we report that the modulation of hns mRNA stability by the endoribonuclease RNase G plays a key role in Salmonella Typhimurium ...
Minho Lee   +10 more
doaj   +2 more sources

Identification, validation, and characterization of approved and investigational drugs interfering with the SARS-CoV-2 endoribonuclease Nsp15. [PDF]

open access: yesProtein Sci
Abstract Since the emergence of SARS‐CoV‐2 at the end of 2019, the virus has caused significant global health and economic disruptions. Despite the rapid development of antiviral vaccines and some approved treatments such as remdesivir and paxlovid, effective antiviral pharmacological treatments for COVID‐19 patients remain limited. This study explores
Chatziefthymiou SD   +13 more
europepmc   +2 more sources

SARS-CoV-2 nsp15 endoribonuclease antagonizes dsRNA-induced antiviral signaling. [PDF]

open access: yesProc Natl Acad Sci U S A
Significance Severe acute respiratory syndrome coronavirus (SARS-CoV)-2 causes a spectrum of respiratory disease ranging from asymptomatic infections to severe pneumonia and death.
Otter CJ   +14 more
europepmc   +2 more sources

Unconventional mRNA processing and degradation pathways for the polycistronic yrzI (spyTA) mRNA in Bacillus subtilis. [PDF]

open access: yesFEBS Lett
The S1025 peptide is the major antidote to the YrzI toxin, which we renamed here as SpyT (Small Peptide YrzI Toxin) and SpyA (Small Peptide YrzI Antitoxin) (1). Degradation of the toxin–antitoxin spyTA mRNA, either by a translation‐dependent cleavage by the endoribonuclease Rae1 (2) or by direct attack by 3′‐exoribonucleases (3), also contributes to ...
Gilet L   +4 more
europepmc   +2 more sources

Functional implications of hexameric dynamics in SARS-CoV-2 Nsp15. [PDF]

open access: yesProtein Sci
Abstract SARS‐CoV‐2, the virus responsible for the COVID‐19 pandemic, has undergone continuous evolution, leading to the emergence of variants with altered transmissibility and immune evasion. For the non‐structural proteins (Nsps) of SARS‐CoV‐2, there are limited structural analyses of their naturally occurring mutations.
Sonowal M   +18 more
europepmc   +2 more sources

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