Results 1 to 10 of about 22,136 (261)

Identification, validation, and characterization of approved and investigational drugs interfering with the SARS-CoV-2 endoribonuclease Nsp15. [PDF]

Protein Sci
Abstract Since the emergence of SARS‐CoV‐2 at the end of 2019, the virus has caused significant global health and economic disruptions. Despite the rapid development of antiviral vaccines and some approved treatments such as remdesivir and paxlovid, effective antiviral pharmacological treatments for COVID‐19 patients remain limited. This study explores
Chatziefthymiou SD, Kuzikov M, Afandi S, Kovacs G, Srivastava S, Zaliani A, Gruzinov A, Pompidor G, Lunelli M, Ahmed GR, Labahn J, Hakanpää J, Windshügel B, Kolbe M.   +13 more
europepmc   +2 more sources

Innate Immune Evasion of PRRSV nsp11 through Degradation of the HDAC2 by Its Endoribonuclease Activity [PDF]

Viruses
Porcine reproductive and respiratory syndrome virus (PRRSV), a member of the Arteriviridae family, represents a persistent menace to the global pig industry, causing reproductive failure and respiratory disease in pigs.
He Zhang, Jianxing Chen, Changqing Yu, Yu Pan, Wenjie Ma, Hao Feng, Jinxin Xie, Hongyan Chen, Yue Wang, Changyou Xia   +9 more
doaj   +2 more sources

An endoribonuclease-based feedforward controller for decoupling resource-limited genetic modules in mammalian cells

Nature Communications, 2020
Accurately predicting the behaviour of a genetic circuit remains difficult due to the lack of modularity. Here the authors quantify the effects of resource loading in mammalian systems and develop an endoribonuclease-based feedfoward controller to adapt ...
Ross D Jones, Breanna Diandreth, Domitilla Del Vecchio   +2 more
exaly   +2 more sources

Cryo-EM structures of the SARS-CoV-2 endoribonuclease Nsp15 reveal insight into nuclease specificity and dynamics

Nature Communications, 2021
Nsp15 is a uridine specific endoribonuclease present in all coronaviruses. Here, the authors determine the cryo-EM structures of SARS-CoV-2 Nsp15 in the apo and UTP-bound states, which together with biochemical experiments, mass spectrometry and ...
Cassandra K Hayne, Monica C Pillon, Meredith N Frazier   +2 more
exaly   +2 more sources

Functional implications of hexameric dynamics in SARS-CoV-2 Nsp15. [PDF]

Protein Sci
Abstract SARS‐CoV‐2, the virus responsible for the COVID‐19 pandemic, has undergone continuous evolution, leading to the emergence of variants with altered transmissibility and immune evasion. For the non‐structural proteins (Nsps) of SARS‐CoV‐2, there are limited structural analyses of their naturally occurring mutations.
Sonowal M, Ketawala G, Nagaratnam N, Logeswaran D, Basu S, de Sanctis D, Orlans J, Rose SL, Jernigan RJ, Hu H, Aguilar JDM, Ranaweera ME, Zacks MA, Chen JJ, Hansen DT, Schrag LG, Fromme R, Botha S, Fromme P.   +18 more
europepmc   +2 more sources

Inhibition of anti-viral stress granule formation by coronavirus endoribonuclease nsp15 ensures efficient virus replication

PLoS Pathogens, 2021
Cytoplasmic stress granules (SGs) are generally triggered by stress-induced translation arrest for storing mRNAs. Recently, it has been shown that SGs exert anti-viral functions due to their involvement in protein synthesis shut off and recruitment of ...
Huan Wang, Ying Liao, Yingjie Sun
exaly   +2 more sources

CryoEM structure of the SLFN14 endoribonuclease reveals insight into RNA binding and cleavage [PDF]

Nature Communications
The SLFN14 endoribonuclease is a post-transcriptional regulator that targets the ribosome and its associated RNA substrates for codon-bias translational repression. SLFN14 nuclease activity is linked to antiviral defense and platelet function.
Justin Van Riper, Arleth O. Martinez-Claros, Lie Wang, Hannah E. Schneiderman, Sweta Maheshwari, Monica C. Pillon   +5 more
doaj   +2 more sources

Tipiracil binds to uridine site and inhibits Nsp15 endoribonuclease NendoU from SARS-CoV-2

Communications Biology, 2021
Youngchang Kim, Jacek Wower, and colleagues explore the sequence specificity, metal ion dependence and catalytic mechanism of the Nsp15 endoribonuclease NendoU from SARS-CoV-2.
Jacek Wower, Natalia Maltseva, Andrzej Joachimiak   +2 more
exaly   +2 more sources

ATF6 Alleviates Endothelial Inflammation Following Extended Hepatectomy Through Inhibition of TRIM10/NF‐κB Signaling [PDF]

FASEB J
Activating transcription factor 6 (ATF6) in liver endothelial cells is upregulated and activated following the extended hepatectomy in both humans and rodents. ATF6 deficiency or pharmacological inhibition of ATF6 leads to severe endothelial inflammation in mice and HUVECs.
Shi C, Yang D, Bai Y, Zhou B, Sun Y, Xu X, Guo W, Zhang S, Liang Y, Jin Y, Shi J.   +10 more
europepmc   +2 more sources

What is the link between stringent response, endoribonuclease encoding Type II Toxin-Antitoxin systems and persistence? [PDF]

Frontiers in Microbiology, 2016
Persistence is a transient and non-inheritable tolerance to antibiotics by a small fraction of a bacterial population. One of the proposed determinants of bacterial persistence is toxin-antitoxin systems (TAS) which are also implicated in a wide range of
Bhaskar Chandra Mohan Ramisetty, Bhaskar Chandra Mohan Ramisetty, Dimpy Ghosh, Moumita Roy Chowdhury, Ramachandran Sarojini Santhosh   +4 more
doaj   +2 more sources